Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,528,110 G→T 8.3% intergenic (‑976/+277) USA300HOU_2385 ← / ← adcA FNT family formate‑nitrite transporter/ABC superfamily ATP binding cassette transporter, binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,528,1100GT 8.3% 322.0 / 10.7 102intergenic (‑976/+277)USA300HOU_2385/adcAFNT family formate‑nitrite transporter/ABC superfamily ATP binding cassette transporter, binding protein
Reads supporting (aligned to +/- strand):  ref base G (41/52);  new base T (7/2);  total (48/54)
Fisher's exact test for biased strand distribution p-value = 7.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACATGCATTGA  >  CP000730/2527971‑2528248
                                                                                                                                           |                                                                                                                                          
aaCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGc                                                                                                                                           <  1:421141/141‑1 (MQ=255)
 aCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATCATGCt                                                                                                                                          >  1:868445/1‑141 (MQ=255)
   cGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCttt                                                                                                                                        >  1:272911/1‑141 (MQ=255)
    tttttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCtttt                                                                                                                                       <  2:610239/140‑1 (MQ=255)
    gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTNGATGAAACCATTNTTCAAATAATGCtttt                                                                                                                                       <  1:730903/141‑1 (MQ=255)
    gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCtttt                                                                                                                                       <  1:786207/141‑1 (MQ=255)
      tttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGt                                                                                                                                     <  1:225998/141‑1 (MQ=255)
            ttCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGtata                                                                                                                               >  2:507368/1‑141 (MQ=255)
              cTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGCCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAgg                                                                                                                             >  2:590696/1‑141 (MQ=255)
                   aaTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                                                                        <  1:794541/141‑1 (MQ=255)
                   aaTGTATAGCTGGTCATTGTAGGTGTTGTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                                                                        <  2:150976/141‑1 (MQ=255)
                    aTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCaa                                                                                                                       >  2:209327/1‑141 (MQ=255)
                    aTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCaa                                                                                                                       >  1:366712/1‑141 (MQ=255)
                      gTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAac                                                                                                                     >  1:525142/1‑141 (MQ=255)
                        ataGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAacac                                                                                                                   <  1:482107/141‑1 (MQ=255)
                         taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                                                                  <  1:1023512/141‑1 (MQ=255)
                         taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                                                                  <  1:205697/141‑1 (MQ=255)
                            cTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCttt                                                                                                               >  1:660753/1‑141 (MQ=255)
                                   ttGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCg                                                                                                        >  2:724492/1‑141 (MQ=255)
                                      tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCACATAATGCTTTTGTCGTATAGGCATCAAACACCTCTTCAATCGGTg                                                                                                     >  2:757046/1‑141 (MQ=255)
                                      tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTg                                                                                                     >  1:353108/1‑141 (MQ=255)
                                      tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATAAATCACCTTTTCAATCGGTg                                                                                                     >  1:826644/1‑141 (MQ=255)
                                        ggTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGCAACCATTTTTCCAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCt                                                                                                   <  2:226852/141‑1 (MQ=255)
                                        ggTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCt                                                                                                   <  2:488853/141‑1 (MQ=255)
                                         gtgtTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCtt                                                                                                  >  2:617892/1‑141 (MQ=255)
                                           gtTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCt                                                                                                <  1:470191/141‑1 (MQ=255)
                                            ttttAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCTTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTa                                                                                               >  2:462877/1‑141 (MQ=255)
                                               tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                                            >  1:271390/1‑141 (MQ=255)
                                               tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAATCCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                                            <  1:715895/141‑1 (MQ=255)
                                               tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                                            <  1:993803/141‑1 (MQ=255)
                                               tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                                            <  2:190226/141‑1 (MQ=255)
                                               tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                                            <  1:249786/141‑1 (MQ=255)
                                                aaTCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTTTTTTGTCGTGTAGGCATCAAACATTTTTTTAATCGGTGTTTCTTTCGt                                                                                           >  2:434163/1‑141 (MQ=255)
                                                aaTCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGt                                                                                           >  2:46564/1‑141 (MQ=255)
                                                aaTCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGt                                                                                           <  1:1063864/141‑1 (MQ=255)
                                                  tCGCATAAAATGCATCACCGCCTGGAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                                                         <  1:897861/141‑1 (MQ=255)
                                                  tCGCATAAAATGCATCACCGCCTGGAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                                                         <  1:897939/141‑1 (MQ=255)
                                                  acGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                                                         <  1:908253/140‑1 (MQ=255)
                                                   cGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCt                                                                                        >  1:389594/1‑141 (MQ=255)
                                                     cATAAAATGCATCACCGCCTGAAACAGCATTAACGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCATTCTTg                                                                                      >  2:582142/1‑141 (MQ=255)
                                                      aTAAAATGCATCACCGCCTGAAACAGCATTAAAGCGACACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGa                                                                                     >  1:786891/1‑141 (MQ=255)
                                                       tAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTATTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCTATCGGTGCttaaaaaaaaaataaa                                                                                    <  2:518378/141‑15 (MQ=255)
                                                       tAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTAATAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTaaaaaaaaaaaa                                                                                    <  2:406214/141‑12 (MQ=255)
                                                          aaTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaa                                                                                 <  2:717632/141‑1 (MQ=255)
                                                           aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                                                                >  1:358462/1‑141 (MQ=255)
                                                             gCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAaata                                                                              >  1:393203/1‑141 (MQ=255)
                                                                tCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                                                                           >  1:1020457/1‑141 (MQ=255)
                                                                  aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTATCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtttt                                                                         <  1:210974/141‑1 (MQ=255)
                                                                  aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTATTCGAAAAATAAtttt                                                                         >  1:332266/1‑141 (MQ=255)
                                                                   ccGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTtta                                                                        <  1:907320/141‑1 (MQ=255)
                                                                    ctccTGAAACAGCATTAAATCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTttat                                                                       <  2:1024663/139‑1 (MQ=255)
                                                                         gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATATTGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                                                                  >  2:651658/1‑141 (MQ=255)
                                                                         gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                                                                  >  2:49160/1‑141 (MQ=255)
                                                                           aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                                                                >  2:429856/1‑141 (MQ=255)
                                                                           aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                                                                >  1:336439/1‑141 (MQ=255)
                                                                           aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                                                                <  2:726621/141‑1 (MQ=255)
                                                                            aCAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCa                                                               <  2:1045680/141‑1 (MQ=255)
                                                                            aCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCa                                                               >  2:623621/1‑141 (MQ=255)
                                                                            aCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTAAAAAAAAAAAAAAAATAATTTTATTATCTTCa                                                               <  2:341694/141‑1 (MQ=255)
                                                                                 aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTTTTTTCACATATAGGCATCAAACACCTTTTCAATCGGTTTTTCTATCGATCTTGTAATCATACTTTTATCATCTACAACtat                                                          >  2:1045045/1‑139 (MQ=255)
                                                                                 aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTTTTTTTTTTTCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACttt                                                          >  2:350338/1‑141 (MQ=255)
                                                                                    aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt                                                       >  1:364319/1‑141 (MQ=255)
                                                                                    aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt                                                       <  1:1061227/141‑1 (MQ=255)
                                                                                     aaacGACACACTTTTGTACTACCCTCTTTTTGATTAAACCATTATTCAATTAATGCTTTTGTCTTATTTTCCTCAAACACCTTTTCAATCGCTACTTCTACAGATCAAAAAAAAATAATTTTATTATCTTCAACTTTAATc                                                      <  2:1015214/138‑1 (MQ=255)
                                                                                      aGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCg                                                     >  1:463584/1‑141 (MQ=255)
                                                                                       gCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGt                                                    >  2:196261/1‑141 (MQ=255)
                                                                                         gAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCa                                                  >  1:253099/1‑141 (MQ=255)
                                                                                           aaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATc                                                <  2:626878/141‑1 (MQ=255)
                                                                                           aaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTATAAAAAAAAAAAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATc                                                <  2:239270/141‑1 (MQ=255)
                                                                                            acacTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCt                                               <  1:630575/141‑1 (MQ=255)
                                                                                              tcTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCTACTTTAATCGTCATCTTc                                             <  2:34019/140‑1 (MQ=255)
                                                                                               cTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTATTATTGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATTGGTGCTTCTATCGTTCTTGAAACAATAATTTTATTATCTTCAACTTTAATCGTCCTCTTcc                                            >  2:11893/1‑141 (MQ=255)
                                                                                                tttGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTccc                                           <  1:116505/141‑1 (MQ=255)
                                                                                                tttGGTACTAGCGTCTTTTGGATGAAACCATTTCTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTccc                                           <  2:126337/141‑1 (MQ=255)
                                                                                                   ggTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACt                                        <  2:547513/141‑1 (MQ=255)
                                                                                                     tACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGTTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTcc                                      >  2:690297/1‑141 (MQ=255)
                                                                                                       cTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCt                                    >  2:177541/1‑141 (MQ=255)
                                                                                                           cGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCt                                <  2:161844/141‑1 (MQ=255)
                                                                                                            gTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCtt                               <  1:906527/141‑1 (MQ=255)
                                                                                                                   ggATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATAtt                        <  2:440257/141‑1 (MQ=255)
                                                                                                                   ggATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATAGTCATCTTCCCTCTCCCTATCTTTTATAtt                        >  1:39755/1‑141 (MQ=255)
                                                                                                                    gATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATAttt                       <  2:237435/141‑1 (MQ=255)
                                                                                                                    gATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATAttt                       >  1:433417/1‑141 (MQ=255)
                                                                                                                      tGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTAc                     >  2:386886/1‑141 (MQ=255)
                                                                                                                      tGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTAc                     <  2:1056789/141‑1 (MQ=255)
                                                                                                                      tGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTAc                     <  2:361103/141‑1 (MQ=255)
                                                                                                                      tGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTAAAAAAAAAAAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTAc                     <  2:919978/141‑1 (MQ=255)
                                                                                                                        aaaCCCTTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCCTCTTTAATCGCCATCTTCCCACTCCCTATCTTTTATATTTACTc                   <  2:358320/141‑1 (MQ=255)
                                                                                                                          aCCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCAt                 <  1:724492/141‑1 (MQ=255)
                                                                                                                             aTTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCtt              >  1:37813/1‑141 (MQ=255)
                                                                                                                              tttttCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTa             >  2:766449/1‑141 (MQ=255)
                                                                                                                                tttCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAAc           <  1:556853/141‑1 (MQ=255)
                                                                                                                                 ttCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTACAAACGGTGCTTATATCCTACTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTTTCTTTTATATTTACTCATCTTAACa          <  1:757554/141‑1 (MQ=255)
                                                                                                                                  tCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACAt         >  1:79886/1‑141 (MQ=255)
                                                                                                                                     aataatGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACATGCa      >  1:906854/1‑141 (MQ=255)
                                                                                                                                     aataatGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACATGCa      >  1:930890/1‑141 (MQ=255)
                                                                                                                                     aataatGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACATGCa      <  1:1035489/141‑1 (MQ=255)
                                                                                                                                     aataatGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACATGCa      >  2:892493/1‑141 (MQ=255)
                                                                                                                                      ataatGCTTTTGTCGTACAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACATGCAt     <  2:206476/141‑1 (MQ=255)
                                                                                                                                         atGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACATGCATTGa  <  1:501274/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTTTATATTTACTCATCTTAACATGCATTGA  >  CP000730/2527971‑2528248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: