Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,590,449 C→T 5.5% Y31Y (TAC→TAT)  USA300HOU_2452 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,590,4490CT 5.5% 284.2 / 4.2 90Y31Y (TAC→TAT) USA300HOU_2452hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (41/44);  new base T (3/2);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

AATATGTGCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTT  >  CP000730/2590309‑2590588
                                                                                                                                            |                                                                                                                                           
aaTATGTGCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTAc                                                                                                                                             <  2:281861/141‑1 (MQ=255)
 atatGTGCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACa                                                                                                                                            >  1:711091/1‑141 (MQ=255)
   atGTGCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAAGAAACATTACATAGAAGAAGAATACGCTACTGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTtatata                                                                                                                                          <  1:971785/141‑1 (MQ=255)
    tgtgCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCTTACGTTTTATTGGATTTATTTTCCTTTACatat                                                                                                                                         >  2:223131/1‑141 (MQ=255)
             gTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTa                                                                                                                                >  1:937553/1‑141 (MQ=255)
             gTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTa                                                                                                                                <  2:410984/141‑1 (MQ=255)
             gTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTa                                                                                                                                >  1:788685/1‑141 (MQ=255)
              tATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTaa                                                                                                                               <  2:298550/141‑1 (MQ=255)
              tATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTaa                                                                                                                               <  2:1026032/141‑1 (MQ=255)
                   atataATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCt                                                                                                                          <  2:657463/141‑1 (MQ=255)
                   atataATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCt                                                                                                                          >  1:452697/1‑141 (MQ=255)
                    tataATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCtt                                                                                                                         >  1:928971/1‑141 (MQ=255)
                      taataCAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGc                                                                                                                       >  2:97111/1‑141 (MQ=255)
                        ataCAACTATTGAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCg                                                                                                                     <  1:1063834/141‑1 (MQ=255)
                          aCAACTATTTAAGCGGTATACGATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGAt                                                                                                                   <  1:947220/141‑1 (MQ=255)
                            aaCTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTTTTTAACATCTTGCCGATct                                                                                                                 >  2:45342/1‑140 (MQ=255)
                            aaCTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCa                                                                                                                 >  2:349385/1‑141 (MQ=255)
                                  ttAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCg                                                                                                           <  1:249314/141‑1 (MQ=255)
                                  ttAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCg                                                                                                           >  1:330153/1‑141 (MQ=255)
                                   tAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGt                                                                                                          <  2:556711/141‑1 (MQ=255)
                                   tAAGACGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGt                                                                                                          <  1:908588/141‑1 (MQ=255)
                                    atgagcgggtaaaTGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTTGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGTCTATCATTATCGTc                                                                                                         >  2:75188/13‑141 (MQ=255)
                                     agagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCt                                                                                                        >  1:531488/1‑141 (MQ=255)
                                     agagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCt                                                                                                        >  1:259248/1‑141 (MQ=255)
                                      gagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtt                                                                                                       >  1:838805/1‑141 (MQ=255)
                                      gagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtt                                                                                                       >  2:725122/1‑141 (MQ=255)
                                      gagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtt                                                                                                       >  1:838806/1‑141 (MQ=255)
                                       agGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttt                                                                                                      <  2:71076/141‑1 (MQ=255)
                                        ggTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtttt                                                                                                     >  1:278102/1‑141 (MQ=255)
                                        ggTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtttt                                                                                                     <  2:1011218/141‑1 (MQ=255)
                                         gTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACTGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCttttt                                                                                                    >  2:264521/1‑141 (MQ=255)
                                         gTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt                                                                                                    <  2:139620/141‑1 (MQ=255)
                                         gTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt                                                                                                    <  2:131145/141‑1 (MQ=255)
                                         gTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt                                                                                                    <  2:814692/141‑1 (MQ=255)
                                          tataCTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTATCTTTTTGCCGATCATTATCGTCTTTTTa                                                                                                   >  2:201201/1‑141 (MQ=255)
                                             aCTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACAGAACACACGAGTATTTTCAGACGAAATATTGTAGTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACt                                                                                                <  1:486931/141‑1 (MQ=255)
                                              cgaTGTCAAATACAAATAAACGTTACATAGAGGAAGAATACGCTACCGAGCAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACGTCTTGCCGATCATTATCGTCTTTTTAACtt                                                                                               <  2:614327/139‑1 (MQ=255)
                                              cTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACtt                                                                                               <  2:526641/141‑1 (MQ=255)
                                               tATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACttt                                                                                              <  2:549104/141‑1 (MQ=255)
                                               tATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACttt                                                                                              <  2:549204/141‑1 (MQ=255)
                                                  gTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTgg                                                                                           >  1:498180/1‑141 (MQ=255)
                                                  gTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTgg                                                                                           >  1:21733/1‑141 (MQ=255)
                                                     aaatacaaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGtt                                                                                        <  1:324977/141‑1 (MQ=255)
                                                      aatacaaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTta                                                                                       >  1:668686/1‑141 (MQ=255)
                                                        tacaaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTtagt                                                                                     <  1:892157/141‑1 (MQ=255)
                                                        tacaaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGGCTGTTTAACGTTGGCGTttgt                                                                                     >  2:488018/1‑141 (MQ=255)
                                                          caaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTtagtag                                                                                   <  1:161800/141‑1 (MQ=255)
                                                           aaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGc                                                                                  <  1:732762/141‑1 (MQ=255)
                                                            aataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGTTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCt                                                                                 >  2:278695/1‑141 (MQ=255)
                                                             ataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTg                                                                                >  1:962549/1‑141 (MQ=255)
                                                             ataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTg                                                                                <  1:534176/141‑1 (MQ=255)
                                                              taAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTAGTTTCCTTTACATGTTGTTGGTTAACATCTTGCCGATGATTATTGTCTTTTTAAATGTTGGGTTAGTAGCTGt                                                                               >  2:189030/1‑141 (MQ=255)
                                                                aaCATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTAt                                                                             >  1:499079/1‑141 (MQ=255)
                                                                aaCATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTAt                                                                             >  2:172488/1‑141 (MQ=255)
                                                                 aCATTACATAGAAGAAGAGTACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTAtt                                                                            <  2:860959/141‑1 (MQ=255)
                                                                 aCATTACATAGAAGAAGAAGACGCTTCCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTATTCAACTTGTGGGTTAGTAGCTGTAtt                                                                            <  1:797820/141‑1 (MQ=255)
                                                                    ttACATAGAAGAAGAATACGCTCCCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCAc                                                                         <  1:879362/141‑1 (MQ=255)
                                                                         tAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTcaac                                                                    <  1:139997/141‑1 (MQ=255)
                                                                          agaagaagaATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTGTTGGGTTAGTAGCTGTATTCACTcaaca                                                                   <  2:347861/141‑1 (MQ=255)
                                                                            aagaagaaTACGCTACCGAACGATCGCGTTTTTTGAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACAGCTTGCCGATCATTATCGTCTTNTTANCTTTNGGGTTAGTANNTGTATTCACTCAACAAg                                                                 <  1:1007079/141‑1 (MQ=255)
                                                                                agaaTACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTcc                                                             >  2:989285/1‑141 (MQ=255)
                                                                                 gaaTACGCTACTGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGcccca                                                            >  1:1051393/1‑137 (MQ=255)
                                                                                 gaaTACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCg                                                            >  1:200974/1‑141 (MQ=255)
                                                                                   aTACGCTTTCGAACTATCGCGTTTTTTCAAACGTGATATTGGATTTGTTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAGCTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTg                                                          <  2:780649/141‑1 (MQ=255)
                                                                                   aTACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTg                                                          <  1:468731/141‑1 (MQ=255)
                                                                                    tACGCTACCGAACAATCGCGTTTTTTCTTTTTTGTTATTGGATTTATTTTCCTTTACATATTTTTGGTTAACTTCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTgg                                                         >  2:966789/1‑141 (MQ=255)
                                                                                       gatcaaaaaCATTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTggcct                                                      <  2:313714/134‑4 (MQ=255)
                                                                                       gCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCACCAAGCTCCGTGGGGt                                                      >  2:805217/1‑141 (MQ=255)
                                                                                       gCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGt                                                      <  2:19530/141‑1 (MQ=255)
                                                                                       gCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTCACCTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGt                                                      <  1:792584/141‑1 (MQ=255)
                                                                                           ccGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTtct                                                  <  1:2470/141‑1 (MQ=255)
                                                                                             gAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTctct                                                >  1:455659/1‑141 (MQ=255)
                                                                                                cAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCtttt                                             >  2:428935/1‑141 (MQ=255)
                                                                                                 aaTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTc                                            >  1:104147/1‑141 (MQ=255)
                                                                                                  aTCTCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTcc                                           <  2:965968/141‑1 (MQ=255)
                                                                                                  aTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCCCCATGGGGTTTCTCTTTTcc                                           <  1:609202/141‑1 (MQ=255)
                                                                                                  aTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTcc                                           <  1:7716/141‑1 (MQ=255)
                                                                                                  aTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTcc                                           <  2:924378/141‑1 (MQ=255)
                                                                                                    cacgtttgaaaaaaCGCGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTa                                         >  1:109706/12‑141 (MQ=255)
                                                                                                     gcgTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTAt                                        <  1:752924/141‑1 (MQ=255)
                                                                                                     acgtttgaaaaaaCGCGATATTGGATTTATTTTCCTTTACATATTTTTGGTTTATATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACACGCTCCGTGCGGTTTCTCTTTTCCTAt                                        >  2:859847/11‑141 (MQ=255)
                                                                                                          ttttCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTTGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGTGTTTCTCTTTTCCTATTTCaa                                   >  2:365637/1‑141 (MQ=255)
                                                                                                          ttttCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCaa                                   <  1:552534/141‑1 (MQ=255)
                                                                                                          tgaaaaaaCGCGATATTGGATTTATTTTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCCCCATGGGGTTTCTCTTTTCCTATTTCaa                                   >  1:1003882/6‑141 (MQ=255)
                                                                                                                     gATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGAGGGGCTTCTCTTTTCCTATTTCAAGGAACATTAGc                        >  1:585685/1‑141 (MQ=255)
                                                                                                                         ttGGATTTATTTTCCTTTACATATTTTTTGTTTACATCTTGCCGATCATTATCGTCTTTTTACCTTTTGGGTTAGTAGCTGTATTCACTCAACACGCTCCGTGGGGTTTCTCTTTTCCTCTTTCAATGACCATTAGCTAtt                    >  2:61605/1‑141 (MQ=255)
                                                                                                                             atttatttTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCCCCATGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACt                <  2:603146/141‑1 (MQ=255)
                                                                                                                             atttatttTCCTTTACAAATTTGTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACt                <  1:58923/141‑1 (MQ=255)
                                                                                                                                  ttttCCTTTACATATTTTTGTTTTATTTCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAg           >  2:522983/1‑141 (MQ=255)
                                                                                                                                  ttttCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAg           <  2:455659/141‑1 (MQ=255)
                                                                                                                                        tttACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGttatta     <  1:865332/141‑1 (MQ=255)
                                                                                                                                           aCATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTAttt  >  1:439740/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
AATATGTGCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTT  >  CP000730/2590309‑2590588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑