Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,590,473 G→A 5.6% P39P (CCG→CCA)  USA300HOU_2452 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,590,4730GA 5.6% 277.1 / 3.5 90P39P (CCG→CCA) USA300HOU_2452hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/47);  new base A (3/2);  total (41/49)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

ATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTCGTCTGATGCA  >  CP000730/2590333‑2590613
                                                                                                                                            |                                                                                                                                            
ataCAACTATTGAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCg                                                                                                                                              <  1:1063834/141‑1 (MQ=255)
  aCAACTATTTAAGCGGTATACGATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGAt                                                                                                                                            <  1:947220/141‑1 (MQ=255)
    aaCTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTTTTTAACATCTTGCCGATct                                                                                                                                          >  2:45342/1‑140 (MQ=255)
    aaCTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCa                                                                                                                                          >  2:349385/1‑141 (MQ=255)
          ttAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCg                                                                                                                                    >  1:330153/1‑141 (MQ=255)
          ttAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCg                                                                                                                                    <  1:249314/141‑1 (MQ=255)
           tAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGt                                                                                                                                   <  2:556711/141‑1 (MQ=255)
           tAAGACGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGt                                                                                                                                   <  1:908588/141‑1 (MQ=255)
            atgagcgggtaaaTGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTTGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGTCTATCATTATCGTc                                                                                                                                  >  2:75188/13‑141 (MQ=255)
             agagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCt                                                                                                                                 >  1:531488/1‑141 (MQ=255)
             agagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCt                                                                                                                                 >  1:259248/1‑141 (MQ=255)
              gagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtt                                                                                                                                >  2:725122/1‑141 (MQ=255)
              gagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtt                                                                                                                                >  1:838806/1‑141 (MQ=255)
              gagGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtt                                                                                                                                >  1:838805/1‑141 (MQ=255)
               agGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttt                                                                                                                               <  2:71076/141‑1 (MQ=255)
                ggTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtttt                                                                                                                              >  1:278102/1‑141 (MQ=255)
                ggTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtttt                                                                                                                              <  2:1011218/141‑1 (MQ=255)
                 gTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACTGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCttttt                                                                                                                             >  2:264521/1‑141 (MQ=255)
                 gTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt                                                                                                                             <  2:131145/141‑1 (MQ=255)
                 gTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt                                                                                                                             <  2:814692/141‑1 (MQ=255)
                 gTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt                                                                                                                             <  2:139620/141‑1 (MQ=255)
                  tataCTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTATCTTTTTGCCGATCATTATCGTCTTTTTa                                                                                                                            >  2:201201/1‑141 (MQ=255)
                     aCTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACAGAACACACGAGTATTTTCAGACGAAATATTGTAGTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACt                                                                                                                         <  1:486931/141‑1 (MQ=255)
                      cgaTGTCAAATACAAATAAACGTTACATAGAGGAAGAATACGCTACCGAGCAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACGTCTTGCCGATCATTATCGTCTTTTTAACtt                                                                                                                        <  2:614327/139‑1 (MQ=255)
                      cTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACtt                                                                                                                        <  2:526641/141‑1 (MQ=255)
                       tATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACttt                                                                                                                       <  2:549204/141‑1 (MQ=255)
                       tATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACttt                                                                                                                       <  2:549104/141‑1 (MQ=255)
                          gTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTgg                                                                                                                    >  1:498180/1‑141 (MQ=255)
                          gTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTgg                                                                                                                    >  1:21733/1‑141 (MQ=255)
                             aaatacaaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGtt                                                                                                                 <  1:324977/141‑1 (MQ=255)
                              aatacaaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTta                                                                                                                >  1:668686/1‑141 (MQ=255)
                                tacaaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTtagt                                                                                                              <  1:892157/141‑1 (MQ=255)
                                tacaaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGGCTGTTTAACGTTGGCGTttgt                                                                                                              >  2:488018/1‑141 (MQ=255)
                                  caaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTtagtag                                                                                                            <  1:161800/141‑1 (MQ=255)
                                   aaataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGc                                                                                                           <  1:732762/141‑1 (MQ=255)
                                    aataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGTTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCt                                                                                                          >  2:278695/1‑141 (MQ=255)
                                     ataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTg                                                                                                         >  1:962549/1‑141 (MQ=255)
                                     ataAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTg                                                                                                         <  1:534176/141‑1 (MQ=255)
                                      taAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTAGTTTCCTTTACATGTTGTTGGTTAACATCTTGCCGATGATTATTGTCTTTTTAAATGTTGGGTTAGTAGCTGt                                                                                                        >  2:189030/1‑141 (MQ=255)
                                        aaCATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTAt                                                                                                      >  1:499079/1‑141 (MQ=255)
                                        aaCATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTAt                                                                                                      >  2:172488/1‑141 (MQ=255)
                                         aCATTACATAGAAGAAGAGTACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTAtt                                                                                                     <  2:860959/141‑1 (MQ=255)
                                         aCATTACATAGAAGAAGAAGACGCTTCCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTATTCAACTTGTGGGTTAGTAGCTGTAtt                                                                                                     <  1:797820/141‑1 (MQ=255)
                                            ttACATAGAAGAAGAATACGCTCCCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCAc                                                                                                  <  1:879362/141‑1 (MQ=255)
                                                 tAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTcaac                                                                                             <  1:139997/141‑1 (MQ=255)
                                                  agaagaagaATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTGTTGGGTTAGTAGCTGTATTCACTcaaca                                                                                            <  2:347861/141‑1 (MQ=255)
                                                    aagaagaaTACGCTACCGAACGATCGCGTTTTTTGAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACAGCTTGCCGATCATTATCGTCTTNTTANCTTTNGGGTTAGTANNTGTATTCACTCAACAAg                                                                                          <  1:1007079/141‑1 (MQ=255)
                                                        agaaTACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTcc                                                                                      >  2:989285/1‑141 (MQ=255)
                                                         gaaTACGCTACTGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGcccca                                                                                     >  1:1051393/1‑137 (MQ=255)
                                                         gaaTACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCg                                                                                     >  1:200974/1‑141 (MQ=255)
                                                           aTACGCTTTCGAACTATCGCGTTTTTTCAAACGTGATATTGGATTTGTTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAGCTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTg                                                                                   <  2:780649/141‑1 (MQ=255)
                                                           aTACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTg                                                                                   <  1:468731/141‑1 (MQ=255)
                                                            tACGCTACCGAACAATCGCGTTTTTTCTTTTTTGTTATTGGATTTATTTTCCTTTACATATTTTTGGTTAACTTCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTgg                                                                                  >  2:966789/1‑141 (MQ=255)
                                                               gatcaaaaaCATTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTggcct                                                                               <  2:313714/134‑4 (MQ=255)
                                                               gCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCACCAAGCTCCGTGGGGt                                                                               >  2:805217/1‑141 (MQ=255)
                                                               gCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGt                                                                               <  2:19530/141‑1 (MQ=255)
                                                               gCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTCACCTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGt                                                                               <  1:792584/141‑1 (MQ=255)
                                                                   ccGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTtct                                                                           <  1:2470/141‑1 (MQ=255)
                                                                     gAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTctct                                                                         >  1:455659/1‑141 (MQ=255)
                                                                        cAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCtttt                                                                      >  2:428935/1‑141 (MQ=255)
                                                                         aaTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTc                                                                     >  1:104147/1‑141 (MQ=255)
                                                                          aTCTCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTcc                                                                    <  2:965968/141‑1 (MQ=255)
                                                                          aTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCCCCATGGGGTTTCTCTTTTcc                                                                    <  1:609202/141‑1 (MQ=255)
                                                                          aTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTcc                                                                    <  1:7716/141‑1 (MQ=255)
                                                                          aTCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTcc                                                                    <  2:924378/141‑1 (MQ=255)
                                                                            cacgtttgaaaaaaCGCGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTa                                                                  >  1:109706/12‑141 (MQ=255)
                                                                             gcgTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTAt                                                                 <  1:752924/141‑1 (MQ=255)
                                                                             acgtttgaaaaaaCGCGATATTGGATTTATTTTCCTTTACATATTTTTGGTTTATATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACACGCTCCGTGCGGTTTCTCTTTTCCTAt                                                                 >  2:859847/11‑141 (MQ=255)
                                                                                  ttttCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTTGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGTGTTTCTCTTTTCCTATTTCaa                                                            >  2:365637/1‑141 (MQ=255)
                                                                                  ttttCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCaa                                                            <  1:552534/141‑1 (MQ=255)
                                                                                  tgaaaaaaCGCGATATTGGATTTATTTTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCCCCATGGGGTTTCTCTTTTCCTATTTCaa                                                            >  1:1003882/6‑141 (MQ=255)
                                                                                             gATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGAGGGGCTTCTCTTTTCCTATTTCAAGGAACATTAGc                                                 >  1:585685/1‑141 (MQ=255)
                                                                                                 ttGGATTTATTTTCCTTTACATATTTTTTGTTTACATCTTGCCGATCATTATCGTCTTTTTACCTTTTGGGTTAGTAGCTGTATTCACTCAACACGCTCCGTGGGGTTTCTCTTTTCCTCTTTCAATGACCATTAGCTAtt                                             >  2:61605/1‑141 (MQ=255)
                                                                                                     atttatttTCCTTTATATATTTTTGGTTAACATCTTGCCAATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCCCCATGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACt                                         <  2:603146/141‑1 (MQ=255)
                                                                                                     atttatttTCCTTTACAAATTTGTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACt                                         <  1:58923/141‑1 (MQ=255)
                                                                                                          ttttCCTTTACATATTTTTGTTTTATTTCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAg                                    >  2:522983/1‑141 (MQ=255)
                                                                                                          ttttCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAg                                    <  2:455659/141‑1 (MQ=255)
                                                                                                                tttACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGttatta                              <  1:865332/141‑1 (MQ=255)
                                                                                                                   aCATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTAttt                           >  1:439740/1‑141 (MQ=255)
                                                                                                                     atatTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCa                         <  2:776075/141‑1 (MQ=255)
                                                                                                                         ttttttttttttttttGCCGATCAATATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACTAGCTCCGTGGGGTTTCTCTTTTTCTTTTTTTTTTTTCTTTATCTTTTTACTCGCAGTTATTATTTCACTAg                     >  2:847046/15‑141 (MQ=255)
                                                                                                                         ttttGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAg                     <  2:1062010/141‑1 (MQ=255)
                                                                                                                         ttttGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAg                     >  2:352407/1‑141 (MQ=255)
                                                                                                                         ttttGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTAATTCACTGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAg                     <  2:226919/141‑1 (MQ=255)
                                                                                                                           ttGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCt                   <  1:12760/141‑1 (MQ=255)
                                                                                                                             ggTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTCGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTTACTAGCTAt                 >  2:174751/1‑141 (MQ=255)
                                                                                                                                  aCATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTc            <  1:321697/141‑1 (MQ=255)
                                                                                                                                  aCATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTc            >  2:1018353/1‑141 (MQ=255)
                                                                                                                                   cATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTCg           <  2:712838/141‑1 (MQ=255)
                                                                                                                                    aTCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTCGt          <  2:166854/141‑1 (MQ=255)
                                                                                                                                        tGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTCGTCTGa      <  1:280488/141‑1 (MQ=255)
                                                                                                                                            gATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTCGTCTGATGCa  <  1:822493/141‑1 (MQ=255)
                                                                                                                                            gATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTCGTCTGATGCa  <  2:259248/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTTTTGGGTTAGTAGCTGTATTCACTCAACAAGCTCCGTGGGGTTTCTCTTTTCCTATTTCAATGAACATTAGCTATTTACTCGCAGTTATTATTTCACTAGCTATTTTTCGTCTGATGCA  >  CP000730/2590333‑2590613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑