Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,614,110	T→G	5.3%	S187S (TCA→TCC)	USA300HOU_2478 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,614,110	0	T	G	5.3%	305.1 / 8.8	94	S187S (TCA→TCC)	USA300HOU_2478	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (56/33);  new base G (3/2);  total (59/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

AAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGG  >  CP000730/2613970‑2614250                                                                                                                                             |                                                                                                                                             aaaTTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCt                                                                                                                                              <  1:974359/141‑1 (MQ=255) aaaTTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCt                                                                                                                                              <  2:872945/141‑1 (MQ=255)       ttGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATaa                                                                                                                                        >  2:911426/1‑141 (MQ=255)        tgtgTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAAc                                                                                                                                       <  1:709886/141‑1 (MQ=255)            tCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTgg                                                                                                                                   <  2:968325/141‑1 (MQ=255)                   gatgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTc                                                                                                                            <  1:843430/141‑1 (MQ=255)                     tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                                          >  2:1018226/1‑141 (MQ=255)                     tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                                          >  1:969394/1‑141 (MQ=255)                     tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                                          >  1:969398/1‑141 (MQ=255)                        tCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGAtt                                                                                                                       >  1:414551/1‑141 (MQ=255)                        tCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGAtt                                                                                                                       >  2:719570/1‑141 (MQ=255)                         ccTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATta                                                                                                                      >  2:23878/1‑141 (MQ=255)                            tttAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTatat                                                                                                                   >  1:1082763/1‑141 (MQ=255)                             ttAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATg                                                                                                                  >  1:534818/1‑141 (MQ=255)                              tAAATCACCATCGCCTTTTATTAATAATTTATGTGCTTTCTTAGTTTGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGa                                                                                                                 <  2:1050642/141‑1 (MQ=255)                               aaaTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGaa                                                                                                                >  2:984254/1‑141 (MQ=255)                                 aTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAAt                                                                                                              >  2:416811/1‑141 (MQ=255)                                  tCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATg                                                                                                             >  2:199428/1‑141 (MQ=255)                                    aCCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGTACATTCGGATTATATGAAATGTc                                                                                                           >  1:54353/1‑141 (MQ=255)                                     ccATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCa                                                                                                          >  1:720590/1‑141 (MQ=255)                                        tCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCa                                                                                                       <  1:394550/141‑1 (MQ=255)                                        tCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCa                                                                                                       >  2:329677/1‑141 (MQ=255)                                          gCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATc                                                                                                     <  2:1035806/141‑1 (MQ=255)                                           ccTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCt                                                                                                    >  1:1017792/1‑141 (MQ=255)                                            cTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCtt                                                                                                   <  1:1023358/141‑1 (MQ=255)                                             ttttATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCttt                                                                                                  <  1:567498/141‑1 (MQ=255)                                                 attaATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATaa                                                                                              >  2:334074/1‑141 (MQ=255)                                                    aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                                           <  2:846648/141‑1 (MQ=255)                                                    aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                                           <  2:351777/141‑1 (MQ=255)                                                    aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                                           <  1:418361/141‑1 (MQ=255)                                                     ataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCAt                                                                                          <  2:862139/141‑1 (MQ=255)                                                       aatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTa                                                                                        <  1:479228/141‑1 (MQ=255)                                                        atTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTaa                                                                                       >  2:244782/1‑141 (MQ=255)                                                          ttAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAata                                                                                     >  1:136658/1‑141 (MQ=255)                                                          ttAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAata                                                                                     >  1:95091/1‑141 (MQ=255)                                                             ggTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCt                                                                                  >  2:854498/1‑141 (MQ=255)                                                              gTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCtt                                                                                 >  2:1043921/1‑141 (MQ=255)                                                              gTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCtt                                                                                 >  1:306952/1‑141 (MQ=255)                                                               agCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCttt                                                                                <  2:402280/140‑1 (MQ=21)                                                                 cTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTaa                                                                              <  2:376506/141‑1 (MQ=255)                                                                    tCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTaaatt                                                                           <  1:603127/141‑3 (MQ=255)                                                                      ttAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCa                                                                         <  2:725275/141‑1 (MQ=255)                                                                       tAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCAc                                                                        >  1:124029/1‑141 (MQ=255)                                                                        aGTTGGTATATTATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCAcc                                                                       >  1:1050600/1‑141 (MQ=255)                                                                           tGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTa                                                                    >  2:60576/1‑141 (MQ=255)                                                                              tataTCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTg                                                                 <  1:592453/141‑1 (MQ=255)                                                                               atattatatctttttCGAAGTTGNTTCACATTGTAATCATCGTTTTTCAATTGATATTTTGCGGAATAACTTGGAACAANAGTATTANATGAAATGNCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                                >  1:565595/13‑141 (MQ=9)                                                                               atatCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                                <  1:105602/141‑1 (MQ=255)                                                                               atatCATACCTCTTTCTAAGTTGCTTCACAGTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                                >  2:975288/1‑141 (MQ=255)                                                                                  tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                                             >  2:948541/1‑141 (MQ=255)                                                                                  tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                                             >  1:159155/1‑141 (MQ=255)                                                                                  tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                                             >  2:66626/1‑141 (MQ=255)                                                                                   cATACCTCTGTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaa                                                            >  1:955571/1‑141 (MQ=255)                                                                                    aTACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaaa                                                           <  2:1030856/141‑1 (MQ=255)                                                                                    aTACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaaa                                                           <  1:137892/141‑1 (MQ=255)                                                                                        ctctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAt                                                       >  2:443322/1‑141 (MQ=255)                                                                                        atctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAt                                                       >  1:1029689/2‑141 (MQ=255)                                                                                            ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATCATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                                   <  1:520836/141‑1 (MQ=255)                                                                                            ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                                   <  2:24184/141‑1 (MQ=255)                                                                                            ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                                   >  1:518574/1‑141 (MQ=255)                                                                                             tCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaaa                                                  >  2:33927/1‑141 (MQ=255)                                                                                              cTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAg                                                 >  1:147351/1‑141 (MQ=255)                                                                                                 aGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGttt                                              <  2:206335/141‑1 (MQ=255)                                                                                                 aGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGttt                                              >  1:796993/1‑141 (MQ=255)                                                                                                    tGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCg                                           <  2:2320/141‑1 (MQ=255)                                                                                                     gCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGa                                          <  1:296031/141‑1 (MQ=255)                                                                                                        tCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAttt                                       >  1:95081/1‑141 (MQ=255)                                                                                                        tCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAttt                                       >  2:822990/1‑141 (MQ=255)                                                                                                          acaTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttct                                     >  2:343694/1‑141 (MQ=255)                                                                                                            aTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttt                                   <  1:909243/141‑1 (MQ=255)                                                                                                            aTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttt                                   >  2:462576/1‑141 (MQ=255)                                                                                                              tGTAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAAAACTTAAAGTCTTCAAtttcttttc                                 <  1:614534/141‑1 (MQ=11)                                                                                                               gTAGTCATCATTACTTTATTGCTACTGTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCTCCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                                <  2:860307/141‑1 (MQ=255)                                                                                                               gTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                                <  2:492826/141‑1 (MQ=255)                                                                                                               gTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                                >  2:602475/1‑141 (MQ=255)                                                                                                               gTAGTCATCATTACTTAATTGATACTATGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                                <  2:860585/141‑1 (MQ=255)                                                                                                                tAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAAAACTTAAAGTCTTCAATttcttttctt                               >  1:350718/1‑141 (MQ=11)                                                                                                                  gTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTaa                             <  1:522233/141‑1 (MQ=255)                                                                                                                    catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt                           >  2:1035091/1‑141 (MQ=255)                                                                                                                    catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt                           >  1:409970/1‑141 (MQ=255)                                                                                                                    catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt                           <  1:911426/141‑1 (MQ=255)                                                                                                                     atcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAttt                          >  1:890444/1‑141 (MQ=255)                                                                                                                     atcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAttt                          >  2:683757/1‑141 (MQ=255)                                                                                                                          tACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTatcat                     >  2:361499/1‑141 (MQ=255)                                                                                                                            cTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTAtcatca                   >  2:1012124/1‑141 (MQ=255)                                                                                                                              tAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGc                 <  1:1078515/141‑1 (MQ=255)                                                                                                                               aaTTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCt                >  2:12694/1‑141 (MQ=255)                                                                                                                               aaTTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCt                >  1:658002/1‑141 (MQ=255)                                                                                                                                    aTACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCgg           >  1:584108/1‑141 (MQ=255)                                                                                                                                     tACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGt          >  1:804759/1‑141 (MQ=255)                                                                                                                                      attttGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAAAACTTAAAGTCTTCAATTTCTTTTCTTAATTTATCATCAGCTATCGGtt         >  1:807783/3‑141 (MQ=17)                                                                                                                                      aCTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGtt         <  2:534818/141‑1 (MQ=255)                                                                                                                                      aCTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGtt         >  2:355830/1‑141 (MQ=255)                                                                                                                                      aCTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGtt         >  2:355918/1‑141 (MQ=255)                                                                                                                                         ttGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTttgg      >  2:765561/1‑141 (MQ=255)                                                                                                                                          tGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTttggt     >  1:971933/1‑141 (MQ=255)                                                                                                                                             tGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTttggttgg  >  1:21742/1‑141 (MQ=255)                                                                                                                                             tGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCATATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTttggttgg  <  2:306952/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                             AAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGG  >  CP000730/2613970‑2614250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑