Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,653,179 C→T 5.8% L584L (CTA→TTA) ‡ fbp → fructose‑bisphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,653,1790CT 5.8% 321.3 / 6.1 104L584L (CTA→TTA) ‡fbpfructose‑bisphosphatase
Reads supporting (aligned to +/- strand):  ref base C (57/41);  new base T (2/4);  total (59/45)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 107 total aligned reads displayed.

AGGACGCATTATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGAT  >  CP000730/2653040‑2653319
                                                                                                                                           |                                                                                                                                            
aGGACGCATTATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACACCTGGCATTGCAGGCTATACACt                                                                                                                                             >  1:282421/1‑141 (MQ=255)
     gCATTATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGt                                                                                                                                        <  1:917188/141‑1 (MQ=255)
       attattAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGtat                                                                                                                                      >  1:150079/1‑141 (MQ=255)
          attaATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAat                                                                                                                                   <  2:532245/141‑1 (MQ=255)
           ttaATGGTCACACACCAGTGAACGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                  >  2:545756/1‑141 (MQ=255)
           ttaATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAATCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                  <  1:679876/141‑1 (MQ=255)
           ttaATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                  >  1:1065637/1‑141 (MQ=255)
           ttaATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                  >  2:196710/1‑141 (MQ=255)
           ttaATGGTCACACACCAGAGAAAGCAAGCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCCACAACTGTCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                  >  2:545794/1‑141 (MQ=255)
              aTGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATAAACTATTGTATAattcat                                                                                                                               >  2:281124/1‑141 (MQ=255)
               tGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAattcatt                                                                                                                              >  1:93817/1‑141 (MQ=255)
                    acacacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTa                                                                                                                         >  1:716817/1‑141 (MQ=255)
                      acacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATg                                                                                                                       <  2:277370/141‑1 (MQ=255)
                       cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                      <  1:452626/141‑1 (MQ=255)
                       cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                      <  2:494143/141‑1 (MQ=255)
                       cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                      <  1:1087215/141‑1 (MQ=255)
                       cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTAAAATTCATTCGGTATGc                                                                                                                      >  1:739652/1‑141 (MQ=255)
                           aGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACt                                                                                                                  >  1:383810/1‑141 (MQ=255)
                            gTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATAGCAGGCTATACACTCTTGTATAATTCATTCGGTATGCAACTc                                                                                                                 >  2:357386/1‑141 (MQ=255)
                             tGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTGTGCAAcgag                                                                                                                <  1:922079/141‑4 (MQ=255)
                             tGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCg                                                                                                                >  2:253318/1‑141 (MQ=255)
                             tGAAAGAAAGCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTGTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTGTGCAAcgag                                                                                                                <  1:675484/141‑4 (MQ=255)
                               aaagaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGtt                                                                                                              >  1:810243/1‑141 (MQ=255)
                               aaagaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGtt                                                                                                              >  1:527532/1‑141 (MQ=255)
                                aagaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACCCTATCGTATAATTCATTCGGTGTGCAAcgagttg                                                                                                             <  2:322833/141‑7 (MQ=255)
                                 agaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGc                                                                                                            >  1:430943/1‑141 (MQ=255)
                                  gaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGca                                                                                                           >  1:477644/1‑141 (MQ=255)
                                   aaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcac                                                                                                          <  2:841308/141‑1 (MQ=255)
                                   aaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcac                                                                                                          <  2:841181/141‑1 (MQ=255)
                                    aaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCATCTCGTTGcaca                                                                                                         <  2:1065244/141‑1 (MQ=255)
                                    aaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcaca                                                                                                         <  2:506950/141‑1 (MQ=255)
                                     aTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCacac                                                                                                        >  1:821402/1‑141 (MQ=255)
                                        aaTGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACcaa                                                                                                     >  2:511657/1‑141 (MQ=255)
                                            gCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAAt                                                                                                 >  2:509460/1‑141 (MQ=255)
                                             cGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAAtt                                                                                                <  1:992094/141‑1 (MQ=255)
                                                 gATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATCCGGTATGCAACTCGTTGCACACCAACAATTTAAc                                                                                            >  1:1007621/1‑141 (MQ=255)
                                                   tCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGc                                                                                          <  1:184240/141‑1 (MQ=255)
                                                   tCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGc                                                                                          <  1:396524/141‑1 (MQ=255)
                                                    ccTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCt                                                                                         >  1:650702/1‑141 (MQ=255)
                                                    ccTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGCATGCAACTCGTTGCACACCAACAATTTAACGCt                                                                                         <  1:563831/141‑1 (MQ=255)
                                                     cTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTa                                                                                        >  1:112038/1‑141 (MQ=255)
                                                       aTCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTaaa                                                                                      >  1:841541/1‑141 (MQ=255)
                                                       aTCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTCTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTaaa                                                                                      >  2:360719/1‑141 (MQ=255)
                                                        tCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAg                                                                                     >  1:400814/1‑141 (MQ=255)
                                                         cAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGa                                                                                    >  2:878024/1‑141 (MQ=255)
                                                           aGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTAAAAAAAAAAAAAATCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACTCCAACTATTTaaaaaaaaaaaaa                                                                                  <  2:965732/141‑12 (MQ=255)
                                                             gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                >  1:139449/1‑141 (MQ=255)
                                                             gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                >  2:1073480/1‑141 (MQ=255)
                                                             gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                >  1:994893/1‑141 (MQ=255)
                                                              cTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGATAAAGaaaaaa                                                                               <  2:183470/141‑1 (MQ=255)
                                                                gATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATa                                                                             >  1:389700/1‑141 (MQ=255)
                                                                gATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCAGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATa                                                                             <  2:739869/141‑1 (MQ=255)
                                                                 aTGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAc                                                                            <  1:607764/141‑1 (MQ=255)
                                                                 aTGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAc                                                                            >  2:401083/1‑141 (MQ=255)
                                                                  tGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACt                                                                           <  2:783879/141‑1 (MQ=255)
                                                                     aaaGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTc                                                                        <  1:295965/141‑1 (MQ=255)
                                                                        gATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGa                                                                     >  1:1069693/1‑141 (MQ=255)
                                                                         aTGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGaa                                                                    <  1:893134/141‑1 (MQ=255)
                                                                               gTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATc                                                              >  1:914229/1‑141 (MQ=255)
                                                                                 cATTGATGATGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGa                                                            <  2:1024374/141‑1 (MQ=255)
                                                                                  attgccggtggCTTTTCAAAAGCTTATCAGGCAACAACTGGCATGGCAGGCTATACACGATTGTATAATTCATTCGGTAGGCAACTCGTTGCACACAAACAATTTAACGCTAATGAAAAATTACTTTCCGAAGATAACGAt                                                           >  2:317162/7‑141 (MQ=255)
                                                                                       tggtggTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGGACGCAACTCGTTGCACACCAACAATTTAACGCTAAAGGAAAAATACTTTCCGAAGGTATCGATGAACt                                                      <  1:289496/141‑1 (MQ=255)
                                                                                       tggtggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTCAAGAAAAAATACTTTCCGAAGGTATCGATGAACt                                                      >  1:1005230/1‑141 (MQ=255)
                                                                                           ggtttttCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                  >  1:718876/4‑141 (MQ=255)
                                                                                           ggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                  >  2:974611/1‑141 (MQ=255)
                                                                                           ggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                  >  2:700321/1‑141 (MQ=255)
                                                                                              ttttCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCtata                                               >  2:197991/1‑141 (MQ=255)
                                                                                              ttttCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCtata                                               >  2:198053/1‑141 (MQ=255)
                                                                                                 tCAAAAGCTTATCAGTCAGCAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAg                                            <  2:540347/141‑1 (MQ=255)
                                                                                                  cAAAAGCTTATCAGTCAACAACTGACATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgc                                           >  2:896439/1‑141 (MQ=255)
                                                                                                   aaaaGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                          <  1:235418/141‑1 (MQ=255)
                                                                                                   aaaaGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                          <  2:210399/141‑1 (MQ=255)
                                                                                                      aGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgt                                       >  2:478865/1‑141 (MQ=255)
                                                                                                        cTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgtcg                                     <  1:527124/141‑1 (MQ=255)
                                                                                                        cTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgtcg                                     >  1:961169/1‑141 (MQ=255)
                                                                                                          tATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTa                                   <  2:841541/141‑1 (MQ=255)
                                                                                                           aTCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAg                                  >  1:359924/1‑141 (MQ=255)
                                                                                                             cAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAATTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGAt                                <  1:520614/141‑1 (MQ=255)
                                                                                                              aGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATa                               <  1:368805/141‑1 (MQ=255)
                                                                                                              aGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATa                               >  1:469566/1‑141 (MQ=255)
                                                                                                                 caacaaCTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAg                            <  1:253318/141‑1 (MQ=255)
                                                                                                                 caacaaCTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAg                            >  2:875976/1‑141 (MQ=255)
                                                                                                                  aacaacTGGCATTGCAGGCTATACACTCTTGTATAATTCATTCGGTATGCAACTCGTTGACCACCAACACTACCACGCTAATGATAAAATCCTTCCCGAAGGTATCGAGGAACTTTCTATAAAGCGCGTCGTAGATAAAGa                           >  1:317664/1‑141 (MQ=255)
                                                                                                                   acaacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGaa                          <  2:1047793/141‑1 (MQ=255)
                                                                                                                    caacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAAt                         <  2:118045/141‑1 (MQ=255)
                                                                                                                    caacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCATCGTAGATAAAGAAt                         >  1:900350/1‑141 (MQ=255)
                                                                                                                         ggCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACaa                    >  1:689036/1‑141 (MQ=255)
                                                                                                                              tGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaa               <  2:199961/141‑1 (MQ=255)
                                                                                                                              tGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaa               >  1:63631/1‑141 (MQ=255)
                                                                                                                                caagCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTTGTAGACAAAGAATTACAACGTaaga             <  2:450374/138‑1 (MQ=255)
                                                                                                                                aaGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTTGTAGACAAAGAATTACAACGTaaga             <  2:450102/140‑1 (MQ=255)
                                                                                                                                 aGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaagaa            <  2:506605/141‑1 (MQ=255)
                                                                                                                                  ggCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaagaag           >  2:485697/1‑141 (MQ=255)
                                                                                                                                   gCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAagaaga          >  2:639607/1‑141 (MQ=255)
                                                                                                                                   gCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAagaaga          >  2:886529/1‑141 (MQ=255)
                                                                                                                                   gCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAagaaga          <  1:635563/141‑1 (MQ=255)
                                                                                                                                      ataCACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTc       >  2:1041701/1‑141 (MQ=255)
                                                                                                                                         cacTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                          >  2:779960/1‑103 (MQ=255)
                                                                                                                                         cacTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                          <  1:779960/103‑1 (MQ=255)
                                                                                                                                           cTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGAt  <  2:590331/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AGGACGCATTATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGAT  >  CP000730/2653040‑2653319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: