Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,654,172 T→A 5.9% G145G (GGT→GGA USA300HOU_2507 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,654,1720TA 5.9% 320.6 / 10.6 102G145G (GGT→GGAUSA300HOU_2507hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base T (45/51);  new base A (2/4);  total (47/55)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01

Only 100 of 104 total aligned reads displayed.

TATTATGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGA  >  CP000730/2654034‑2654309
                                                                                                                                          |                                                                                                                                         
tattatGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGgtgt                                                                                                                                         >  1:688337/1‑141 (MQ=255)
tattatGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTCAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGgtgt                                                                                                                                         >  1:103248/1‑141 (MQ=255)
     tGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTAccc                                                                                                                                    <  2:350235/141‑1 (MQ=255)
       ccTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGGTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCa                                                                                                                                  <  2:1011840/140‑1 (MQ=255)
        cTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAt                                                                                                                                 >  1:298549/1‑141 (MQ=255)
          gCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAttt                                                                                                                               >  1:401847/1‑141 (MQ=255)
           cAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAtttt                                                                                                                              >  1:780533/1‑141 (MQ=255)
           cAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAtttt                                                                                                                              >  2:116568/1‑141 (MQ=255)
            aGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAttttt                                                                                                                             >  2:736614/1‑141 (MQ=255)
             ggtggtGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTa                                                                                                                            >  1:886586/1‑141 (MQ=255)
             ggaggtgCGTCAACCCTTCCTCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTa                                                                                                                            <  2:73686/138‑1 (MQ=255)
                  tgCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGc                                                                                                                       >  2:698159/1‑141 (MQ=255)
                    cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     >  1:724541/1‑141 (MQ=255)
                    cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     <  1:356072/141‑1 (MQ=255)
                    cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     >  1:622895/1‑141 (MQ=255)
                    cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     >  1:622767/1‑141 (MQ=255)
                     gTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGAt                                                                                                                    >  1:738066/1‑141 (MQ=255)
                       cAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTg                                                                                                                  >  2:137458/1‑141 (MQ=255)
                         aCCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTc                                                                                                                >  2:282334/1‑141 (MQ=255)
                            aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                             >  1:84525/1‑141 (MQ=255)
                            aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                             <  2:533735/141‑1 (MQ=255)
                             ttCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTa                                                                                                            <  1:442719/141‑1 (MQ=255)
                              tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCCACCCTGTTTCTACAATTCGATCGTTTCATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:488338/141‑1 (MQ=255)
                              tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:947317/141‑1 (MQ=255)
                              tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:991300/141‑1 (MQ=255)
                                   aaaGTTTCAACTTACCGTATTGGCTGAGCGCTCTCATATTAGTCGTCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTg                                                                                                      <  2:126801/141‑1 (MQ=255)
                                     aGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCg                                                                                                    >  1:625725/1‑141 (MQ=255)
                                         tCAACTTGCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                >  1:861031/1‑141 (MQ=255)
                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      >  2:822589/1‑135 (MQ=255)
                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      <  1:822697/135‑1 (MQ=255)
                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      <  1:822589/135‑1 (MQ=255)
                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      >  2:822697/1‑135 (MQ=255)
                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                <  2:684092/141‑1 (MQ=255)
                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                >  1:178647/1‑141 (MQ=255)
                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACAATGTTTCTAACATTAGATAGTTTAATTGTTGTGCTTGGAGGTGTTAATCCATTTTTACTTGCGATTGTCATTTTGATTGAGGAct                                                                                                >  1:593740/1‑141 (MQ=255)
                                          cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTcta                                                                                               >  2:768659/1‑141 (MQ=255)
                                          cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGActa                                                                                               >  1:539437/1‑141 (MQ=255)
                                              ttACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAt                                                                                           >  1:444396/1‑141 (MQ=255)
                                                aCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGTTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAttt                                                                                         <  2:1031287/141‑1 (MQ=255)
                                                aCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAttt                                                                                         >  1:775293/1‑141 (MQ=255)
                                                     aTTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaa                                                                                    <  1:990426/141‑1 (MQ=255)
                                                      ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTCATTGCGATTGTCATTATGATTGCGGGCTACTATTTCacaac                                                                                   >  2:106226/1‑141 (MQ=255)
                                                      ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaac                                                                                   >  2:325646/1‑141 (MQ=255)
                                                           tGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTc                                                                              <  1:285221/141‑1 (MQ=255)
                                                             aGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCAt                                                                            >  2:969614/1‑141 (MQ=255)
                                                                  aCTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGa                                                                       >  2:76066/1‑141 (MQ=255)
                                                                       tatTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTa                                                                  <  2:139240/141‑1 (MQ=255)
                                                                       tatTAGTCGCCTTTATTTTAGCAACACTGTTTATAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTa                                                                  >  1:134888/1‑141 (MQ=255)
                                                                        atTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTAc                                                                 <  2:964018/141‑1 (MQ=255)
                                                                        atTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTAc                                                                 <  1:999250/141‑1 (MQ=255)
                                                                          tAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTg                                                               <  2:1080244/141‑1 (MQ=255)
                                                                           aGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGc                                                              <  1:201361/141‑1 (MQ=255)
                                                                             tCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCg                                                            <  1:422127/141‑1 (MQ=255)
                                                                               gCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCt                                                          <  1:733766/141‑1 (MQ=255)
                                                                               gCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCt                                                          >  1:819666/1‑141 (MQ=255)
                                                                                ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                         <  1:1046421/141‑1 (MQ=255)
                                                                                ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                         <  1:410927/141‑1 (MQ=255)
                                                                                ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtc                                                         <  1:1030610/141‑2 (MQ=255)
                                                                                    tATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtaataa                                                     <  2:467099/141‑1 (MQ=255)
                                                                                     aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                    >  2:806459/1‑141 (MQ=255)
                                                                                     aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                    >  2:300783/1‑141 (MQ=255)
                                                                                      ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                   <  1:917231/141‑1 (MQ=255)
                                                                                      ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                   >  2:166751/1‑141 (MQ=255)
                                                                                        ttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgat                                                 <  1:766676/141‑1 (MQ=255)
                                                                                         tagaaaCAGTGTTGCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgatg                                                >  1:228137/5‑141 (MQ=255)
                                                                                          aGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGc                                               <  1:385709/141‑1 (MQ=255)
                                                                                           gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              >  2:592010/1‑141 (MQ=255)
                                                                                           gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              >  1:707687/1‑141 (MQ=255)
                                                                                           gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              <  1:724916/141‑1 (MQ=255)
                                                                                            cAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTc                                             <  2:724541/141‑1 (MQ=255)
                                                                                              acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                           <  2:411678/141‑1 (MQ=255)
                                                                                              acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGACTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                           <  2:539437/141‑1 (MQ=255)
                                                                                                 cTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGAtt                                        >  1:662063/1‑141 (MQ=255)
                                                                                                    tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                     <  1:944274/141‑1 (MQ=255)
                                                                                                    tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                     <  1:311521/141‑1 (MQ=255)
                                                                                                     ttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATa                                    >  2:1085392/1‑141 (MQ=255)
                                                                                                      tCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATaa                                   <  1:518813/141‑1 (MQ=255)
                                                                                                         aaaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCa                                <  2:602062/141‑1 (MQ=255)
                                                                                                              tCGCTCGTTTAATTTCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAAt                           <  2:340212/141‑1 (MQ=255)
                                                                                                               cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                          <  1:415019/141‑1 (MQ=255)
                                                                                                               cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                          >  2:451789/1‑141 (MQ=255)
                                                                                                                  tCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                       >  1:315445/1‑141 (MQ=255)
                                                                                                                  tCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                       >  1:496211/1‑141 (MQ=255)
                                                                                                                  tCGTTTAATCGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                       >  1:953068/1‑141 (MQ=255)
                                                                                                                   cGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTa                      >  2:105480/1‑141 (MQ=255)
                                                                                                                    gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                     <  2:1014765/141‑1 (MQ=255)
                                                                                                                        aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTCCTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                 <  2:501313/141‑1 (MQ=255)
                                                                                                                        aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                 <  1:619872/141‑1 (MQ=255)
                                                                                                                          ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg               >  2:566229/1‑141 (MQ=255)
                                                                                                                          ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg               <  2:625967/141‑1 (MQ=255)
                                                                                                                           tGCTGTTCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTTAGATTCATAAGCAGAAATCATTATCACCTgg              <  2:327658/141‑1 (MQ=255)
                                                                                                                           tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATGATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg              <  2:933884/141‑1 (MQ=255)
                                                                                                                           tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg              >  2:993280/1‑141 (MQ=255)
                                                                                                                             cTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAt            <  1:785981/141‑1 (MQ=255)
                                                                                                                              tgtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtg           >  2:162629/1‑141 (MQ=255)
                                                                                                                                 gCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtggtg        <  1:880663/141‑1 (MQ=255)
                                                                                                                                   ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  1:282334/141‑1 (MQ=255)
                                                                                                                                   ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  2:586348/141‑1 (MQ=255)
                                                                                                                                   ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  1:251530/141‑1 (MQ=255)
                                                                                                                                       cGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGa  >  1:194854/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TATTATGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGA  >  CP000730/2654034‑2654309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: