Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,654,193 G→A 5.5% A152A (GCG→GCA USA300HOU_2507 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,654,1930GA 5.5% 340.7 / 5.4 108A152A (GCG→GCAUSA300HOU_2507hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (45/57);  new base A (2/4);  total (47/61)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 108 total aligned reads displayed.

CGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAGCTTGC  >  CP000730/2654054‑2654332
                                                                                                                                           |                                                                                                                                           
cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                                            >  1:724541/1‑141 (MQ=255)
cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                                            >  1:622767/1‑141 (MQ=255)
cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                                            >  1:622895/1‑141 (MQ=255)
 gTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGAt                                                                                                                                           >  1:738066/1‑141 (MQ=255)
   cAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTg                                                                                                                                         >  2:137458/1‑141 (MQ=255)
     aCCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTc                                                                                                                                       >  2:282334/1‑141 (MQ=255)
        aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                                                    <  2:533735/141‑1 (MQ=255)
        aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                                                    >  1:84525/1‑141 (MQ=255)
         ttCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTa                                                                                                                                   <  1:442719/141‑1 (MQ=255)
          tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCCACCCTGTTTCTACAATTCGATCGTTTCATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                                                  <  1:488338/141‑1 (MQ=255)
          tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                                                  <  1:947317/141‑1 (MQ=255)
          tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                                                  <  1:991300/141‑1 (MQ=255)
               aaaGTTTCAACTTACCGTATTGGCTGAGCGCTCTCATATTAGTCGTCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTg                                                                                                                             <  2:126801/141‑1 (MQ=255)
                 aGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCg                                                                                                                           >  1:625725/1‑141 (MQ=255)
                     tCAACTTGCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                                       >  1:861031/1‑141 (MQ=255)
                     tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                                             >  2:822697/1‑135 (MQ=255)
                     tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                                             <  1:822697/135‑1 (MQ=255)
                     tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                                             <  1:822589/135‑1 (MQ=255)
                     tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                                             >  2:822589/1‑135 (MQ=255)
                     tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                                       >  1:178647/1‑141 (MQ=255)
                     tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                                       <  2:684092/141‑1 (MQ=255)
                     tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACAATGTTTCTAACATTAGATAGTTTAATTGTTGTGCTTGGAGGTGTTAATCCATTTTTACTTGCGATTGTCATTTTGATTGAGGAct                                                                                                                       >  1:593740/1‑141 (MQ=255)
                      cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTcta                                                                                                                      >  2:768659/1‑141 (MQ=255)
                      cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGActa                                                                                                                      >  1:539437/1‑141 (MQ=255)
                          ttACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAt                                                                                                                  >  1:444396/1‑141 (MQ=255)
                            aCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGTTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAttt                                                                                                                <  2:1031287/141‑1 (MQ=255)
                                 aTTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaa                                                                                                           <  1:990426/141‑1 (MQ=255)
                                  ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTCATTGCGATTGTCATTATGATTGCGGGCTACTATTTCacaac                                                                                                          >  2:106226/1‑141 (MQ=255)
                                  ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaac                                                                                                          >  2:325646/1‑141 (MQ=255)
                                       tGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTc                                                                                                     <  1:285221/141‑1 (MQ=255)
                                         aGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCAt                                                                                                   >  2:969614/1‑141 (MQ=255)
                                              aCTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGa                                                                                              >  2:76066/1‑141 (MQ=255)
                                                   tatTAGTCGCCTTTATTTTAGCAACACTGTTTATAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTa                                                                                         >  1:134888/1‑141 (MQ=255)
                                                    atTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTAc                                                                                        <  1:999250/141‑1 (MQ=255)
                                                    atTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTAc                                                                                        <  2:964018/141‑1 (MQ=255)
                                                      tAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTg                                                                                      <  2:1080244/141‑1 (MQ=255)
                                                       aGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGc                                                                                     <  1:201361/141‑1 (MQ=255)
                                                         tCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCg                                                                                   <  1:422127/141‑1 (MQ=255)
                                                           gCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCt                                                                                 <  1:733766/141‑1 (MQ=255)
                                                           gCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCt                                                                                 >  1:819666/1‑141 (MQ=255)
                                                            ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                                                <  1:1046421/141‑1 (MQ=255)
                                                            ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                                                <  1:410927/141‑1 (MQ=255)
                                                            ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtc                                                                                <  1:1030610/141‑2 (MQ=255)
                                                                tATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtaataa                                                                            <  2:467099/141‑1 (MQ=255)
                                                                 aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                                           >  2:806459/1‑141 (MQ=255)
                                                                 aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                                           >  2:300783/1‑141 (MQ=255)
                                                                  ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                                          <  1:917231/141‑1 (MQ=255)
                                                                  ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                                          >  2:166751/1‑141 (MQ=255)
                                                                    ttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgat                                                                        <  1:766676/141‑1 (MQ=255)
                                                                     tagaaaCAGTGTTGCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgatg                                                                       >  1:228137/5‑141 (MQ=255)
                                                                      aGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGc                                                                      <  1:385709/141‑1 (MQ=255)
                                                                       gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                                                     >  1:707687/1‑141 (MQ=255)
                                                                       gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                                                     <  1:724916/141‑1 (MQ=255)
                                                                       gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                                                     >  2:592010/1‑141 (MQ=255)
                                                                          acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                                                  <  2:411678/141‑1 (MQ=255)
                                                                          acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGACTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                                                  <  2:539437/141‑1 (MQ=255)
                                                                             cTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGAtt                                                               >  1:662063/1‑141 (MQ=255)
                                                                                tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                                            <  1:311521/141‑1 (MQ=255)
                                                                                tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                                            <  1:944274/141‑1 (MQ=255)
                                                                                 ttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATa                                                           >  2:1085392/1‑141 (MQ=255)
                                                                                  tCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATaa                                                          <  1:518813/141‑1 (MQ=255)
                                                                                     aaaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTCCCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCa                                                       <  1:942558/141‑1 (MQ=255)
                                                                                     aaaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCa                                                       <  2:602062/141‑1 (MQ=255)
                                                                                          tCGCTCGTTTAATTTCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAAt                                                  <  2:340212/141‑1 (MQ=255)
                                                                                           cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                                                 <  1:415019/141‑1 (MQ=255)
                                                                                              tCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                                              >  1:315445/1‑141 (MQ=255)
                                                                                              tCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                                              >  1:496211/1‑141 (MQ=255)
                                                                                               cGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTa                                             >  2:105480/1‑141 (MQ=255)
                                                                                                gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                                            <  1:53838/141‑1 (MQ=255)
                                                                                                gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                                            <  2:1014765/141‑1 (MQ=255)
                                                                                                    aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTCCTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                                        <  2:501313/141‑1 (MQ=255)
                                                                                                    aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                                        <  1:619872/141‑1 (MQ=255)
                                                                                                      ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg                                      <  2:625967/141‑1 (MQ=255)
                                                                                                      ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg                                      >  2:566229/1‑141 (MQ=255)
                                                                                                       tGCTGTTCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTTAGATTCATAAGCAGAAATCATTATCACCTgg                                     <  2:327658/141‑1 (MQ=255)
                                                                                                       tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATGATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg                                     <  2:933884/141‑1 (MQ=255)
                                                                                                       tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg                                     >  2:993280/1‑141 (MQ=255)
                                                                                                         cTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAt                                   <  1:785981/141‑1 (MQ=255)
                                                                                                          tgtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtg                                  >  2:162629/1‑141 (MQ=255)
                                                                                                             gCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtggtg                               <  1:880663/141‑1 (MQ=255)
                                                                                                               ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt                             <  2:586348/141‑1 (MQ=255)
                                                                                                               ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt                             <  1:251530/141‑1 (MQ=255)
                                                                                                               ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt                             <  1:282334/141‑1 (MQ=255)
                                                                                                                   cGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGa                         >  1:194854/1‑141 (MQ=255)
                                                                                                                         tACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGAt                   <  2:865494/141‑1 (MQ=255)
                                                                                                                         tACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGAt                   <  2:650460/141‑1 (MQ=255)
                                                                                                                          aCCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGAtt                  <  1:928208/141‑1 (MQ=255)
                                                                                                                             ccATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAAc               >  2:211708/1‑141 (MQ=255)
                                                                                                                               aTTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTa             <  2:444396/141‑1 (MQ=255)
                                                                                                                                 ttttAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATg           <  1:1002283/141‑1 (MQ=255)
                                                                                                                                 ttttAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATg           <  2:84525/141‑1 (MQ=255)
                                                                                                                                    tAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCaa        <  1:516979/141‑1 (MQ=255)
                                                                                                                                     aaTTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAg       >  2:1017541/1‑141 (MQ=255)
                                                                                                                                     aaTTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAg       >  2:429304/1‑141 (MQ=255)
                                                                                                                                     aaTTACGATTGTCATTATGATTGCGGTCTCCTATTTCACAACATGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGCTGGTGCATTGCTGCAATAAACTATACAAg       >  2:702658/1‑141 (MQ=255)
                                                                                                                                       ttGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAGCt     <  2:893832/141‑1 (MQ=255)
                                                                                                                                       ttGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAGCt     >  2:13913/1‑141 (MQ=255)
                                                                                                                                         gCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAGCTTg   >  2:42452/1‑141 (MQ=255)
                                                                                                                                         gCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAGCTTg   <  1:566229/141‑1 (MQ=255)
                                                                                                                                         gCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAGCTTg   >  2:462062/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATTAACTATGCAAGCTTGC  >  CP000730/2654054‑2654332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: