BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,721,481	T→C	10.9%	L198S (TTA→TCA)	USA300HOU_2575 →	hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,721,481	0	T	C	10.9%	270.9 / 19.7	108	L198S (TTA→TCA)	USA300HOU_2575	hydrolase
Reads supporting (aligned to +/- strand): ref base T (58/38); new base C (6/6); total (64/44)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AACAGAAGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAAT  >  CP000730/2721344‑2721617

aaCAGAAGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGa                                                                                                                                       >  2:926265/1‑141 (MQ=255)

 aCAGAAGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGaa                                                                                                                                      <  1:406189/141‑1 (MQ=255)

  cAGAAGGCTTAGAAAAAGGCATGAAAGCAGTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGCCGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAAt                                                                                                                                     <  1:198787/141‑1 (MQ=255)

   aGAAGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAAtt                                                                                                                                    >  1:967242/1‑141 (MQ=255)

   aGAAGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAAtt                                                                                                                                    <  2:555220/141‑1 (MQ=255)

    gAAGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAAttt                                                                                                                                   <  2:383862/141‑1 (MQ=255)

     aaGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTg                                                                                                                                  >  1:90275/1‑141 (MQ=255)

     aaGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTg                                                                                                                                  >  2:1059725/1‑141 (MQ=255)

         cTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGAATAGAACAGTACAAACGTACGATGTTATGGTTAGCAttttcaat                                                                                                                              >  2:856705/1‑136 (MQ=255)

          ttAGAAAAAGGTATGAAAACATTTGGTGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAAtt                                                                                                                             >  1:386567/1‑141 (MQ=255)

          atAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAAtt                                                                                                                             <  2:272111/140‑1 (MQ=255)

           tAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTa                                                                                                                            >  1:1072778/1‑141 (MQ=255)

            aGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAg                                                                                                                           >  2:248056/1‑141 (MQ=255)

              aaaaaGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGAc                                                                                                                         >  2:42085/1‑141 (MQ=255)

                 aaGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAat                                                                                                                      >  2:20731/1‑141 (MQ=255)

                  aGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAata                                                                                                                     <  2:528172/141‑1 (MQ=255)

                   ggCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAatat                                                                                                                    >  1:547077/1‑141 (MQ=255)

                   ggCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAatat                                                                                                                    >  1:1054454/1‑141 (MQ=255)

                        gAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCa                                                                                                               <  2:237749/141‑1 (MQ=255)

                         aaaaCATTTAGCGAAACATTAAATAACGCACCAATTGATGCAAAAATGATATCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATAAGACAACAAACGCag                                                                                                              >  2:1069823/1‑140 (MQ=255)

                            aCATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCa                                                                                                           <  2:830974/141‑1 (MQ=255)

                            aCATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCa                                                                                                           >  2:73644/1‑141 (MQ=255)

                             cATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaa                                                                                                          >  2:616591/1‑141 (MQ=255)

                             cATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaa                                                                                                          >  2:826063/1‑141 (MQ=255)

                              aTTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAACCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaaa                                                                                                         <  1:39094/141‑1 (MQ=255)

                              aTTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaaa                                                                                                         <  1:877568/141‑1 (MQ=255)

                              aTTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaaa                                                                                                         >  1:779737/1‑141 (MQ=255)

                               tttGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAat                                                                                                        >  2:21451/1‑141 (MQ=255)

                               tttGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCagat                                                                                                        >  1:125603/1‑138 (MQ=255)

                                ttGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAata                                                                                                       <  2:912212/141‑1 (MQ=255)

                                  ggtgAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATAtt                                                                                                     >  2:706548/4‑141 (MQ=255)

                                  ggCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATAtt                                                                                                     <  2:800633/141‑1 (MQ=255)

                                  ggCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATAtt                                                                                                     >  1:837035/1‑141 (MQ=255)

                                       aaCATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACttt                                                                                                >  2:657482/1‑141 (MQ=255)

                                        aCATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTg                                                                                               >  2:844075/1‑141 (MQ=255)

                                            tAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCATATATTACTTTGgatg                                                                                           >  2:378175/1‑141 (MQ=255)

                                             aaaTATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGgatga                                                                                          >  1:838853/1‑141 (MQ=255)

                                              aaTATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGgatgat                                                                                         <  2:595421/141‑1 (MQ=255)

                                                 atCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTc                                                                                      >  2:363292/1‑141 (MQ=255)

                                                  tCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTca                                                                                     >  2:752031/1‑141 (MQ=255)

                                                  tCGCACCAATTGAGGCAAAAATGATGTCTCAACCTGGTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTGca                                                                                     <  2:122552/141‑1 (MQ=255)

                                                   cGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTcac                                                                                    <  2:1081226/141‑1 (MQ=255)

                                                    gCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTcaca                                                                                   >  1:59621/1‑141 (MQ=255)

                                                      aCCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACaaa                                                                                 >  1:304755/1‑141 (MQ=255)

                                                       ccAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTATGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACaaaa                                                                                >  2:1015493/1‑141 (MQ=255)

                                                        cAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAAt                                                                               >  2:783018/1‑141 (MQ=255)

                                                           ttGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACa                                                                            <  1:873715/141‑1 (MQ=255)

                                                             gATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGc                                                                          >  2:869755/1‑141 (MQ=255)

                                                              aTGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACaaaa                                                                                <  2:643313/134‑1 (MQ=255)

                                                               tGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTCCAAACGTACGATGTTCTGGTCAGATTTAGAAATTAGACAATATACGCATTCAGATATTACTTTAGATGATATCACAAAATACAGCGa                                                                        >  2:411321/1‑141 (MQ=255)

                                                               tGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGa                                                                        <  2:75528/141‑1 (MQ=255)

                                                                 cAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATa                                                                      <  2:763634/141‑1 (MQ=255)

                                                                       tgatgTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCa                                                                <  2:1065958/141‑1 (MQ=255)

                                                                          tgTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCAccc                                                             <  2:555564/141‑1 (MQ=255)

                                                                            tctcAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCcta                                                           <  1:81137/141‑1 (MQ=255)

                                                                             ctcAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCctac                                                          <  1:965079/141‑1 (MQ=255)

                                                                                aaCCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTaa                                                       >  1:75692/1‑141 (MQ=255)

                                                                                 aCCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTaaa                                                      >  1:771320/1‑141 (MQ=255)

                                                                                     gctgATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACGAAATggg                                                  >  1:1038353/1‑141 (MQ=255)

                                                                                      ctgATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGa                                                 <  2:696629/141‑1 (MQ=255)

                                                                                        gATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACa                                               <  2:386567/141‑1 (MQ=255)

                                                                                         aTACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAg                                              <  2:96335/141‑1 (MQ=255)

                                                                                            ccgaagagGGACGTATAGAACAGTACAAACGTGCGGCGTTCTTGTTAGAATTCGATATTAGACAATATCCGCACTCCAATAATACTTTGGATGCTTTAACAATATACAGCGATAAAATCACCCTACTAAACGAGAcccagt                                           >  2:556513/3‑136 (MQ=255)

                                                                                               aagaagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTa                                        >  2:416878/1‑141 (MQ=255)

                                                                                                 gaagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTaga                                      >  1:309292/1‑141 (MQ=255)

                                                                                                 gaagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTaga                                      >  1:846434/1‑141 (MQ=255)

                                                                                                  aagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTagag                                     >  1:289087/1‑141 (MQ=255)

                                                                                                   agGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAgg                                    >  1:722942/1‑141 (MQ=255)

                                                                                                    ggACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGc                                   <  1:369191/141‑1 (MQ=255)

                                                                                                    ggACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGc                                   >  1:134109/1‑141 (MQ=255)

                                                                                                     gACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCt                                  <  2:171287/141‑1 (MQ=255)

                                                                                                      aCGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTc                                 >  2:2258/1‑141 (MQ=255)

                                                                                                       cGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTcc                                <  1:797328/141‑1 (MQ=255)

                                                                                                       cGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTcc                                >  2:351317/1‑141 (MQ=255)

                                                                                                          ataGATCAGGACAAAGGTATGATTTTCTTGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTc                             <  2:973063/141‑1 (MQ=255)

                                                                                                          ataGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTc                             >  2:880354/1‑141 (MQ=255)

                                                                                                          ataGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTc                             <  1:675065/141‑1 (MQ=255)

                                                                                                             gAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCca                          >  1:243751/1‑141 (MQ=255)

                                                                                                             gAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCca                          <  2:967242/141‑1 (MQ=255)

                                                                                                              aaCAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCcac                         <  1:646780/141‑1 (MQ=255)

                                                                                                               aCAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCcaca                        <  2:304755/141‑1 (MQ=255)

                                                                                                                  gTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGa                     <  1:1052132/141‑1 (MQ=255)

                                                                                                                    aCAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATg                   >  2:286770/1‑141 (MQ=255)

                                                                                                                    aCAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATg                   <  2:366879/141‑1 (MQ=255)

                                                                                                                     cAAACGTACGATGTTCTGGTTATAATTTCAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGTACAGATTCTAGATGCTCCTTCCCACAAGATGt                  <  1:1015493/141‑1 (MQ=255)

                                                                                                                     cAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGt                  <  1:476618/141‑1 (MQ=255)

                                                                                                                       aaCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTa                >  1:14345/1‑141 (MQ=255)

                                                                                                                       aaCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTa                >  2:62187/1‑141 (MQ=255)

                                                                                                                       aaCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTa                <  1:142275/141‑1 (MQ=255)

                                                                                                                       aaCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTa                >  1:894221/1‑141 (MQ=255)

                                                                                                                         cGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAAc              >  2:109403/1‑141 (MQ=255)

                                                                                                                         cGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAAc              >  2:1063641/1‑141 (MQ=255)

                                                                                                                         cGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCGACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAAc              <  2:968187/141‑1 (MQ=255)

                                                                                                                          gTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACt             >  1:515096/1‑141 (MQ=255)

                                                                                                                            aCGATGTTCTGGTTAGAGTTTGAAATTCGACAATATACGCATTCAAATATTACTTTGGATGAATTCACAAAATACAGCGATAAAATCACCCTACTATATGGGACAGAATCTAGGGGCTCATTCCCACTAGATGTTAACttt           >  2:159364/1‑141 (MQ=255)

                                                                                                                            aCGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACttt           >  1:135728/1‑141 (MQ=255)

                                                                                                                               aTGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCAAAAAATACAGCGATAAAATAACCCTACTAAATGGGACAGATTCTAGAGGCTCCGTTCCACCAGATGTTAACTTTtat        >  1:327636/1‑141 (MQ=255)

                                                                                                                                 gTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTtatat      >  1:171892/1‑141 (MQ=255)

                                                                                                                                   tCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATataa    <  2:425434/141‑1 (MQ=255)

                                                                                                                                     tGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATataaat  >  2:315654/1‑141 (MQ=255)

AACAGAAGGCTTAGAAAAAGGCATGAAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAAT  >  CP000730/2721344‑2721617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`