Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 18,223 G→A 7.5% V278I (GTT→ATT)  USA300HOU_0013 → possible membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073018,2230GA 7.5% 270.7 / 7.1 90V278I (GTT→ATT) USA300HOU_0013possible membrane protein
Reads supporting (aligned to +/- strand):  ref base G (48/35);  new base A (4/3);  total (52/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAACCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTCCACT  >  CP000730/18083‑18362
                                                                                                                                            |                                                                                                                                            
gTCAACCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTgg                                                                                                                                              <  2:248855/141‑1 (MQ=255)
  cAACCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGtt                                                                                                                                            >  1:595230/1‑141 (MQ=255)
    aCCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTAt                                                                                                                                          <  2:511336/141‑1 (MQ=255)
     ccGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATc                                                                                                                                         >  1:830889/1‑141 (MQ=255)
        aGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGt                                                                                                                                      >  2:20457/1‑141 (MQ=255)
         gtgtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGtc                                                                                                                                     >  2:7600/1‑140 (MQ=255)
         gtgtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTa                                                                                                                                     >  1:598861/1‑141 (MQ=255)
         gtgtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGAGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTa                                                                                                                                     >  2:741233/1‑141 (MQ=255)
           gtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACa                                                                                                                                   >  2:55679/1‑141 (MQ=255)
           gtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACa                                                                                                                                   <  2:1041195/141‑1 (MQ=255)
            tgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACaa                                                                                                                                  >  1:390044/1‑141 (MQ=255)
            tgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACaa                                                                                                                                  <  2:414334/141‑1 (MQ=255)
                   gagCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGac                                                                                                                           >  2:622168/1‑141 (MQ=255)
                     gCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGacac                                                                                                                         >  2:643272/1‑141 (MQ=255)
                       aTTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTTGTTATCGGTACAATACTGACACCt                                                                                                                       >  2:609520/1‑141 (MQ=255)
                          cTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACg                                                                                                                    >  1:281245/1‑141 (MQ=255)
                          cTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACg                                                                                                                    <  2:440360/141‑1 (MQ=255)
                              tAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGacac                                                                                                               <  2:310570/141‑1 (MQ=255)
                                    tCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTg                                                                                                         >  2:239018/1‑141 (MQ=255)
                                     ccAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGt                                                                                                        >  1:393860/1‑141 (MQ=255)
                                          ttGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGac                                                                                                   >  1:350367/1‑141 (MQ=255)
                                            gtgtTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTTTAGGacta                                                                                                 >  2:1085225/1‑141 (MQ=255)
                                            gtgtTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGacta                                                                                                 >  1:658855/1‑141 (MQ=255)
                                              gtTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGactact                                                                                               <  2:533744/141‑1 (MQ=255)
                                              gtTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGactact                                                                                               >  1:144658/1‑141 (MQ=255)
                                               ttATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAGGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACtt                                                                                              >  2:372866/1‑141 (MQ=255)
                                                tATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTg                                                                                             >  2:450280/1‑141 (MQ=255)
                                                 aTCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTgg                                                                                            <  1:190811/141‑1 (MQ=255)
                                                 aTCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTgg                                                                                            >  2:786199/1‑141 (MQ=255)
                                                 aTCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGACGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATATTGACACCTACGACACATATTGTATGACTACTTgg                                                                                            >  2:832848/1‑141 (MQ=255)
                                                   cATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGgtg                                                                                          >  2:954009/1‑141 (MQ=255)
                                                      tAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTa                                                                                       >  1:1033467/1‑141 (MQ=255)
                                                      tAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTa                                                                                       <  1:17225/141‑1 (MQ=255)
                                                          aaTGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATTGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGa                                                                                   <  1:550136/141‑1 (MQ=255)
                                                          aaTGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGa                                                                                   <  1:605329/141‑1 (MQ=255)
                                                           aTGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGAt                                                                                  >  2:311493/1‑141 (MQ=255)
                                                             gTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGAttt                                                                                >  1:429571/1‑141 (MQ=255)
                                                             gTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGAttt                                                                                <  2:617162/141‑1 (MQ=255)
                                                             gTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGAttt                                                                                <  2:72291/141‑1 (MQ=255)
                                                              tataTTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTa                                                                               <  1:906752/141‑1 (MQ=255)
                                                              tataTTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTa                                                                               <  1:169520/141‑1 (MQ=255)
                                                                 atTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGt                                                                            >  1:159587/1‑141 (MQ=255)
                                                                  ttCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGtt                                                                           <  1:451654/141‑1 (MQ=255)
                                                                            aaGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGtttgaatttgaa                                                                 <  1:978095/141‑1 (MQ=255)
                                                                              gtgtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGc                                                               >  2:473027/1‑141 (MQ=255)
                                                                              gtgtTATTCAGTTCTTTGTGAAAGCGAAATGTTGGCCGAAGGCAGTGACGATTTTACTATTGGTTATCGGTACAATACGGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGc                                                               <  1:217879/141‑1 (MQ=255)
                                                                               tgtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCg                                                              <  1:205364/141‑1 (MQ=255)
                                                                                gtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTTACTTTGAAGCGa                                                             <  2:153594/141‑1 (MQ=255)
                                                                                gtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGa                                                             <  1:131223/141‑1 (MQ=255)
                                                                                 ttATTCATTTTTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGaa                                                            <  1:965446/141‑1 (MQ=255)
                                                                                  tATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAAt                                                           <  1:20457/141‑1 (MQ=255)
                                                                                     tCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTGGAAGCGAATCAt                                                        >  2:742006/1‑141 (MQ=255)
                                                                                        tttCTTTGGTAAAGCGAAAGGTTTGCAGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACCACAATTGTAGGACTACTTGGTGTTATAGATTTAAGTTTGAATTTGAAGCGAATCATGaa                                                     >  1:431645/1‑141 (MQ=255)
                                                                                         ttCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGCATCATGaaa                                                    >  1:60110/1‑141 (MQ=255)
                                                                                             ttGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAaata                                                >  1:893749/1‑141 (MQ=255)
                                                                                             ttGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAaata                                                >  1:362504/1‑141 (MQ=255)
                                                                                             ttGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAaata                                                >  1:175096/1‑141 (MQ=255)
                                                                                             ttGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAaata                                                >  2:184384/1‑141 (MQ=255)
                                                                                              tGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAaataa                                               <  2:297812/141‑1 (MQ=255)
                                                                                                gTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAAtt                                             >  2:685379/1‑141 (MQ=255)
                                                                                                gTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAAtt                                             <  1:538893/141‑1 (MQ=255)
                                                                                                gTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAAtt                                             >  1:463672/1‑141 (MQ=255)
                                                                                                  aaaGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCt                                           <  2:779802/141‑1 (MQ=255)
                                                                                                   aaGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGACGCGAATCATGAAAaataaatata                                          <  2:185965/141‑6 (MQ=255)
                                                                                                   aaGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTa                                          >  2:668780/1‑141 (MQ=255)
                                                                                                      cGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTaaaa                                       <  2:892911/141‑1 (MQ=255)
                                                                                                        aaaGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAg                                     <  1:741233/141‑1 (MQ=255)
                                                                                                         aaGGTTTGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTAGAATTTGAAGCGAATCATGAAAAATAATTTNAAAAAGt                                    >  2:816074/1‑141 (MQ=255)
                                                                                                          aGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTg                                   >  2:637222/1‑141 (MQ=255)
                                                                                                            gTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGaa                                 <  2:727931/141‑1 (MQ=255)
                                                                                                              ttGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATa                               <  1:740785/141‑1 (MQ=255)
                                                                                                              ttGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATa                               <  1:1040818/141‑1 (MQ=255)
                                                                                                                 ccGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATagag                            <  1:686326/141‑1 (MQ=255)
                                                                                                                 ccGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAAAAATTATAAAAAGTGAATagag                            <  2:595230/141‑1 (MQ=255)
                                                                                                                 ccGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATagag                            >  2:97677/1‑141 (MQ=255)
                                                                                                                     aTGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTgg                        >  1:666672/1‑141 (MQ=255)
                                                                                                                      tGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGa                       <  1:1043999/141‑1 (MQ=255)
                                                                                                                         aGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGaata                    <  1:254425/141‑1 (MQ=255)
                                                                                                                          gTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGaataa                   >  2:1054389/1‑141 (MQ=255)
                                                                                                                          gTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGaataa                   >  2:619250/1‑141 (MQ=255)
                                                                                                                           tAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGaataat                  <  1:977179/141‑1 (MQ=255)
                                                                                                                                aTTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATc             <  2:666672/141‑1 (MQ=255)
                                                                                                                                 ttttACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCg            >  2:13943/1‑141 (MQ=255)
                                                                                                                                 ttttACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCg            >  2:946878/1‑141 (MQ=255)
                                                                                                                                 ttttACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCg            >  1:664873/1‑141 (MQ=255)
                                                                                                                                  tttACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCgg           >  1:59596/1‑141 (MQ=255)
                                                                                                                                  tttACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCgg           >  2:171408/1‑141 (MQ=255)
                                                                                                                                   ttACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGc          >  1:341728/1‑141 (MQ=255)
                                                                                                                                   ttACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGc          >  2:491570/1‑141 (MQ=255)
                                                                                                                                    tACTATTGGTTATCGGTACAATACTGACACCTACGACAAATAATGTCGGAGCCCTTGGTGTTTACGACTGCAGTTTGCCGTTGAAGCTAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCa         <  1:918444/141‑1 (MQ=255)
                                                                                                                                         ttGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCACGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTCCa    >  2:232884/1‑141 (MQ=255)
                                                                                                                                           ggTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTCCACt  <  1:12025/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GTCAACCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTCCACT  >  CP000730/18083‑18362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: