Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 178,248 T→G 7.8% Y75D (TAT→GAT)  cap5E → capsular polysaccharide biosynthesis protein Cap5E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730178,2480TG 7.8% 262.7 / 13.2 90Y75D (TAT→GAT) cap5Ecapsular polysaccharide biosynthesis protein Cap5E
Reads supporting (aligned to +/- strand):  ref base T (36/47);  new base G (4/3);  total (40/50)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

TATTAAAGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTCATATGT  >  CP000730/178109‑178388
                                                                                                                                           |                                                                                                                                            
tatTAAAGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAAGAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTtta                                                                                                                                             <  1:565286/141‑1 (MQ=255)
 atTAAAGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTttat                                                                                                                                            <  2:653311/141‑1 (MQ=255)
    aaaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTAc                                                                                                                                         >  1:804499/1‑141 (MQ=255)
    aaaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTAc                                                                                                                                         >  1:695718/1‑141 (MQ=255)
    aaaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTAc                                                                                                                                         >  1:696779/1‑141 (MQ=255)
    aaaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAAATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTAc                                                                                                                                         >  2:196605/1‑141 (MQ=255)
     aaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACg                                                                                                                                        <  1:1064438/141‑1 (MQ=255)
      aGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGt                                                                                                                                       <  2:686624/141‑1 (MQ=255)
      aGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGt                                                                                                                                       >  2:502332/1‑141 (MQ=255)
       gAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTa                                                                                                                                      >  1:570127/1‑141 (MQ=255)
          aTTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTc                                                                                                                                   <  2:987141/141‑1 (MQ=255)
           ttCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTcc                                                                                                                                  <  2:675849/141‑1 (MQ=255)
           ttCGTATTTTTTCACGCAATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAGATTAATGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAATAGCGATGCGAGATGTTTATTACGTATTcc                                                                                                                                  <  2:675696/141‑1 (MQ=255)
                 ttttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGGTGTGCGTGATAGTCTATGTGTAGAAACTGCAATCCGAGATGTTTATTACGTATTCCATgcag                                                                                                                            <  2:313294/141‑1 (MQ=255)
                 ttttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATgcag                                                                                                                            >  1:800174/1‑141 (MQ=255)
                  tttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATgcagc                                                                                                                           <  2:26486/141‑1 (MQ=255)
                  tttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATgcagc                                                                                                                           <  2:960115/141‑1 (MQ=255)
                   ttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATgcagca                                                                                                                          >  2:579967/1‑141 (MQ=255)
                      tCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCt                                                                                                                       <  2:851327/141‑1 (MQ=255)
                        tcatcgcgtgaAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGGGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCttt                                                                                                                     >  1:932356/10‑141 (MQ=255)
                        aCGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCttt                                                                                                                     >  2:485565/1‑141 (MQ=255)
                         cgcgATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTa                                                                                                                    >  2:751839/1‑141 (MQ=255)
                          gcgATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTaa                                                                                                                   >  1:553078/1‑141 (MQ=255)
                                         aaGATGACATTCGAAAAAAATATAATAAATCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCAt                                                                                                    >  2:594760/1‑141 (MQ=255)
                                          aGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTATAAAGCAAGTGCCGTCAtg                                                                                                   >  1:772178/1‑141 (MQ=255)
                                          aGATGACATACGAAAAAAATATAATAATTCAAAATTAAAGTTCAACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAACACAAGTGCCGTCAtg                                                                                                   <  2:495891/141‑1 (MQ=255)
                                           gATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCAtgt                                                                                                  >  2:785248/1‑141 (MQ=255)
                                            aTGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCAtgtg                                                                                                 <  1:495084/141‑1 (MQ=255)
                                             agACAATCAAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGa                                                                                                <  2:703609/140‑1 (MQ=255)
                                               aCATTCGAAAAAAATATAATAATTCAGAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAAt                                                                                              <  2:253390/141‑1 (MQ=255)
                                               aCATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGGAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTGATGTGAAt                                                                                              >  2:574249/1‑141 (MQ=255)
                                                 aTTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTc                                                                                            <  2:51230/141‑1 (MQ=255)
                                                 aTTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTc                                                                                            <  1:489857/141‑1 (MQ=255)
                                                   tCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCtt                                                                                          <  2:849385/141‑1 (MQ=255)
                                                   acGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCtt                                                                                          <  2:459108/140‑1 (MQ=255)
                                                    cGAAAAAAATATAATGAGTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCttt                                                                                         <  2:776862/141‑1 (MQ=255)
                                                      aaaaaaaTATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTcc                                                                                       <  2:1048347/141‑1 (MQ=255)
                                                        aaaaaaATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAg                                                                                     >  1:576583/1‑141 (MQ=255)
                                                          aaaTATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGtt                                                                                   >  2:117715/1‑141 (MQ=255)
                                                           aaTATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTg                                                                                  <  2:647273/141‑1 (MQ=255)
                                                            atatAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGa                                                                                 <  1:130830/141‑1 (MQ=255)
                                                            atatAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGa                                                                                 <  2:842043/141‑1 (MQ=255)
                                                            atatAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGa                                                                                 <  1:558561/141‑1 (MQ=255)
                                                              ataataATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAgg                                                                               <  2:800174/141‑1 (MQ=255)
                                                              ataataATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTACAGTTGAgg                                                                               >  1:274813/1‑141 (MQ=255)
                                                                        aaaaTTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGAc                                                                     <  2:1032120/141‑1 (MQ=255)
                                                                        aaaaTTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAg                                                                                                             >  1:877597/1‑101 (MQ=255)
                                                                        aaaaTTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAg                                                                                                             <  2:877597/101‑1 (MQ=255)
                                                                         aaaTTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACa                                                                    <  2:691749/141‑1 (MQ=255)
                                                                           aTTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACaaa                                                                  <  1:57833/141‑1 (MQ=255)
                                                                            ttAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAat                                                                 >  1:443217/1‑141 (MQ=255)
                                                                             tAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAata                                                                <  2:387602/141‑1 (MQ=255)
                                                                                aGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAAtatta                                                             <  2:525843/141‑1 (MQ=255)
                                                                                   tCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTg                                                          <  2:259491/141‑1 (MQ=255)
                                                                                    cTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTgg                                                         <  2:116943/141‑1 (MQ=255)
                                                                                     tACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGt                                                        <  1:132579/141‑1 (MQ=255)
                                                                                          tGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGa                                                   <  2:162299/141‑1 (MQ=255)
                                                                                               aTGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATg                                              <  2:933736/141‑1 (MQ=255)
                                                                                                 gtgCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTc                                            >  2:948929/1‑141 (MQ=255)
                                                                                                  tgCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCt                                           <  2:511512/141‑1 (MQ=255)
                                                                                                    cGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTa                                         >  1:64452/1‑141 (MQ=255)
                                                                                                     gTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTAc                                        >  1:259269/1‑141 (MQ=255)
                                                                                                        aTAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACaaa                                     >  1:45035/1‑141 (MQ=255)
                                                                                                         tAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAg                                    <  2:851371/141‑1 (MQ=255)
                                                                                                           cattacattagatgaCAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCATCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTg                                  <  2:586975/127‑1 (MQ=255)
                                                                                                            tCACAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGc                                 <  1:325607/141‑1 (MQ=255)
                                                                                                             cAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCt                                >  1:1025660/1‑141 (MQ=255)
                                                                                                               aaGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAt                              >  1:251301/1‑141 (MQ=255)
                                                                                                               aaGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAt                              <  2:707020/141‑1 (MQ=255)
                                                                                                               aaGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAACGTCTTACAAAGTGCTAt                              >  2:317933/1‑141 (MQ=255)
                                                                                                                aGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAtt                             >  2:764167/1‑141 (MQ=255)
                                                                                                                aGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAtt                             >  2:65444/1‑141 (MQ=255)
                                                                                                                aGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAtt                             >  1:468947/1‑141 (MQ=255)
                                                                                                                 gtgtAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtc                            <  1:1004136/141‑1 (MQ=255)
                                                                                                                 gtgtAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtc                            <  2:387943/141‑1 (MQ=255)
                                                                                                                 gtgtAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtc                            <  1:624118/141‑1 (MQ=255)
                                                                                                                 gtgtAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtc                            <  2:292049/141‑1 (MQ=255)
                                                                                                                   gtAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtcat                          <  1:502077/141‑1 (MQ=255)
                                                                                                                     aGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtcatca                        >  1:670579/1‑141 (MQ=255)
                                                                                                                       aaaCAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCaaa                      >  1:630505/1‑141 (MQ=255)
                                                                                                                        aaCAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCaaaa                     >  2:600570/1‑141 (MQ=255)
                                                                                                                         aCAGCAATGCGAGATGTTTATTGCGTAGTCCGTGCAGGAGGTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAAt                    <  2:363457/141‑1 (MQ=255)
                                                                                                                           aGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGt                  >  1:99644/1‑141 (MQ=255)
                                                                                                                           aGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGt                  <  1:632226/141‑1 (MQ=255)
                                                                                                                           aGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGt                  >  1:366400/1‑141 (MQ=255)
                                                                                                                            gCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGtt                 <  1:407794/141‑1 (MQ=255)
                                                                                                                                 gCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTaaaaa            >  1:294653/1‑141 (MQ=255)
                                                                                                                                  cGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTaaaaaa           >  1:125544/1‑141 (MQ=255)
                                                                                                                                    agaTGTTTATTACGTATTCCATGCAGCAGCATTAAAAAAAGTGCCGTCATGTGAATTCTTTCCAGAAGAGGCAGTGAAGACAAATATTATTGGTACAAAAAATGTCTTACAAATTGCTATTCAAAAAAAAAAAAAAAAAGt         <  2:236699/141‑1 (MQ=255)
                                                                                                                                    agaTGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGAGCTATTCATCAAAATGTTAAAAAAGt         >  2:860601/1‑141 (MQ=255)
                                                                                                                                     gaTGTTTATTACGTATTCCATGCAGCAGCTGTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTc        >  2:975225/1‑141 (MQ=255)
                                                                                                                                     gaTGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGtagaactttaa        <  2:443217/141‑11 (MQ=255)
                                                                                                                                          ttattaCGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTCATATg   >  2:517969/1‑141 (MQ=255)
                                                                                                                                          ttattaCGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTCATATg   <  2:570127/141‑1 (MQ=255)
                                                                                                                                           tattaCGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTGCCAGTAGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAAAAAAAAAAAAGTCATATGt  <  2:932356/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TATTAAAGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTCATATGT  >  CP000730/178109‑178388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: