Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 238,173 T→C 6.1% I58I (ATT→ATC USA300HOU_0214 → oligopeptide ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730238,1730TC 6.1% 264.1 / 3.7 90I58I (ATT→ATCUSA300HOU_0214oligopeptide ABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base T (37/47);  new base C (3/3);  total (40/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAACGT  >  CP000730/238034‑238308
                                                                                                                                           |                                                                                                                                       
aaTTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAattatta                                                                                                                                        <  2:183103/141‑1 (MQ=255)
  ttattCGCAATGTACGCCATATCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAattattatt                                                                                                                                      <  2:1022395/141‑1 (MQ=255)
   tattCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTAttt                                                                                                                                     <  1:889128/141‑1 (MQ=255)
     ttCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTa                                                                                                                                   >  2:532942/1‑141 (MQ=255)
     ttCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTa                                                                                                                                   >  2:796638/1‑141 (MQ=255)
     ttCGCAAGTTACGCCATAGCAACTTATATTTTAGTTAGATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTa                                                                                                                                   >  1:786039/1‑141 (MQ=255)
      tCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTaa                                                                                                                                  >  1:420265/1‑141 (MQ=255)
            gTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACgg                                                                                                                            <  1:732109/141‑1 (MQ=255)
            gTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACgg                                                                                                                            <  1:732118/141‑1 (MQ=255)
            gTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACgg                                                                                                                            <  1:343532/141‑1 (MQ=255)
              tACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaa                                                                                                                          <  2:931715/141‑1 (MQ=255)
               aCGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTTTTTTTTTTTTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaaa                                                                                                                         >  2:397092/1‑140 (MQ=255)
               aCGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaac                                                                                                                         <  2:67319/141‑1 (MQ=255)
                cGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaaca                                                                                                                        <  2:565768/141‑1 (MQ=255)
                cGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaaca                                                                                                                        <  2:1011595/141‑1 (MQ=255)
                 gCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaacaa                                                                                                                       >  1:834163/1‑141 (MQ=255)
                 gCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaacaa                                                                                                                       >  1:1043509/1‑141 (MQ=255)
                  ccATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaacaaa                                                                                                                      <  1:724572/141‑1 (MQ=255)
                  ccATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaacaaa                                                                                                                      <  2:356937/141‑1 (MQ=255)
                          aCTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAg                                                                                                              <  1:820751/141‑1 (MQ=255)
                           cTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGc                                                                                                             <  2:86113/141‑1 (MQ=255)
                            ttATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGca                                                                                                            >  1:33657/1‑141 (MQ=255)
                             tataTTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGcat                                                                                                           >  1:1065936/1‑141 (MQ=255)
                               tattttatttTTATTCACGAGTGCATTACATCTTTTTAAAGGTTATGTGCCCGTTACGTTCTTTGTTGCTGAAACTTTGCTAATCGTTTTTACCATCATTTTAATTATTATTTTAACGACGGAACAAACATGGAAGcatca                                                                                                         <  1:454793/141‑1 (MQ=255)
                                atTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGcatcat                                                                                                        <  2:605191/141‑1 (MQ=255)
                                 ttttAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATg                                                                                                       >  2:821842/1‑141 (MQ=255)
                                   ttAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGAc                                                                                                     <  1:392952/141‑1 (MQ=255)
                                    tAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAATCATGGAAGCATCATGAcc                                                                                                    <  2:61757/141‑1 (MQ=255)
                                    tAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGAcc                                                                                                    <  2:925180/141‑1 (MQ=255)
                                    tAGTTATATCAACGAGGGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAACCATGGAAGCATCATGAcc                                                                                                    <  1:113029/141‑1 (MQ=255)
                                     aGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGActt                                                                                                   >  1:718054/1‑139 (MQ=255)
                                       ttATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTAt                                                                                                 >  1:504853/1‑141 (MQ=255)
                                        tataTTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGTAGCATCATGACCTATg                                                                                                >  2:384579/1‑141 (MQ=255)
                                           atTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCg                                                                                             <  1:781508/141‑1 (MQ=255)
                                           atTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCg                                                                                             <  2:669664/141‑1 (MQ=255)
                                                 gggttcATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCTACGTAt                                                                                       <  1:976765/136‑1 (MQ=255)
                                                 gAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTAt                                                                                       <  2:997935/141‑1 (MQ=255)
                                                     gCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTAtcgtc                                                                                   >  2:333879/1‑141 (MQ=255)
                                                          aaaTCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAgt                                                                              <  1:1009913/141‑1 (MQ=255)
                                                            aTCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTAAGACGACGTATCGTCGAAgtgt                                                                            <  1:255837/141‑1 (MQ=255)
                                                             tCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGtgtt                                                                           <  2:8599/141‑1 (MQ=255)
                                                               tttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGtgttgt                                                                         <  2:445081/141‑1 (MQ=255)
                                                                ttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTgttgtt                                                                        >  2:793165/1‑141 (MQ=255)
                                                                ttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTgttgtt                                                                        <  2:1003057/141‑1 (MQ=255)
                                                                ttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGACGAAGTgttgtt                                                                        >  2:688442/1‑141 (MQ=255)
                                                                ttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATGTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTgttgtt                                                                        <  1:43901/141‑1 (MQ=255)
                                                                    aaaGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATTGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTAttg                                                                    <  2:793739/141‑1 (MQ=255)
                                                                     aaGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCGTCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTAttgt                                                                   >  2:790640/1‑141 (MQ=255)
                                                                     aaGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGAGCTATGGCGACGTATCGTCGAAGTGTTGTTAttgt                                                                   >  1:1003997/1‑141 (MQ=255)
                                                                      aGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTAttgtt                                                                  <  1:120008/141‑1 (MQ=255)
                                                                          tATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTtgatg                                                              >  2:877945/1‑141 (MQ=255)
                                                                          tATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTtgatg                                                              <  2:379217/141‑1 (MQ=255)
                                                                            tgtgGCCGATACGTTCTATATTGCTGAAACATTGCTAATCATTTTAACCATCATTTTACTTCTCATTTTAACAACGGAACAAACATGGCAGCATCATGACCTATGGCGACGTATCGTCGAAGGGTTGTTATTGTtgatggc                                                            >  1:620255/1‑139 (MQ=255)
                                                                               ggCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACAtt                                                         >  1:578604/1‑141 (MQ=255)
                                                                                   gATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTAatgtcatcaacaataaca                                                     <  1:917174/141‑15 (MQ=255)
                                                                                       cGTTCTATATTGCTGAAACATTGCTAATCATTTTAACCATCATTTTAATTATCATTTTAACAACGGAACAAACATGGAAGcatcat                                                                                                        >  2:837889/1‑86 (MQ=255)
                                                                                       cGTTCTATATTGCTGAAACATTGCTAATCATTTTAACCATCATTTTAATTATCATTTTAACAACGGAACAAACATGGAAGcatcat                                                                                                        <  1:837889/86‑1 (MQ=255)
                                                                                       cGTTCTATATTGCTGAAACATTGCTAATCATTTTAACCATCATTTTAATTATCATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGGCATTAACAGGCa                                                 >  1:675156/1‑141 (MQ=255)
                                                                                        gTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCaa                                                >  2:281301/1‑141 (MQ=255)
                                                                                        gTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCaa                                                <  2:130178/141‑1 (MQ=255)
                                                                                        gTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACAATAACAGGCaa                                                >  1:275541/1‑141 (MQ=255)
                                                                                          tCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACg                                              <  1:645258/141‑1 (MQ=255)
                                                                                            tataTTGCGGAAACATTGCTAATCATTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTa                                            <  2:213181/141‑1 (MQ=255)
                                                                                             atatTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTAt                                           >  1:1000686/1‑141 (MQ=255)
                                                                                             atatTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTAt                                           <  2:143012/141‑1 (MQ=255)
                                                                                                ttGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATCATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTa                                        <  1:851749/141‑1 (MQ=255)
                                                                                                    tGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTa                                                                                                                                   <  2:288140/46‑1 (MQ=255)
                                                                                                    tGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTa                                                                                                                                   >  1:288140/1‑46 (MQ=255)
                                                                                                    tGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATTACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACatt                                    <  1:423844/141‑1 (MQ=255)
                                                                                                           ttGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATg                             >  1:779735/1‑141 (MQ=255)
                                                                                                            tGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGt                            >  1:189494/1‑141 (MQ=255)
                                                                                                             gCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCCACGTCTTTACATTATTAATGtt                           >  2:575172/1‑141 (MQ=255)
                                                                                                               tAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTg                         >  1:359399/1‑141 (MQ=255)
                                                                                                               tAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTg                         <  2:1041893/141‑1 (MQ=255)
                                                                                                                    gTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGt                    <  1:857670/141‑1 (MQ=255)
                                                                                                                    gTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGt                    <  2:880758/141‑1 (MQ=255)
                                                                                                                    gTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGt                    >  1:438070/1‑141 (MQ=255)
                                                                                                                     ttttAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTa                   >  1:75475/1‑141 (MQ=255)
                                                                                                                      tgtAACCAGCGTTTTAAGTAGCATTGTAACAGCGGAACAAACAGGGAAGCATCATGACGTATGGCGACGTATCGTCGGAGTGTTGTTATTGTTGATGGCATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTAt                  <  2:620255/139‑1 (MQ=255)
                                                                                                                       ttAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTAtt                 <  1:968360/141‑1 (MQ=255)
                                                                                                                       ttAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTAtt                 <  1:379285/141‑1 (MQ=255)
                                                                                                                        tAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTa                >  2:764338/1‑141 (MQ=255)
                                                                                                                        tAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTa                >  2:741371/1‑141 (MQ=255)
                                                                                                                          aCCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGa              >  1:1026385/1‑141 (MQ=255)
                                                                                                                          aCCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGa              >  1:375738/1‑141 (MQ=255)
                                                                                                                          aCCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGa              >  1:159539/1‑141 (MQ=255)
                                                                                                                           ccATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGAc             <  1:592703/141‑1 (MQ=255)
                                                                                                                           ccATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGAc             <  2:786039/141‑1 (MQ=255)
                                                                                                                             atcatTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGt           <  2:488583/141‑1 (MQ=255)
                                                                                                                                 ttttAATTATTATTTTAACAACGGAACAAACACGGAAGCATCATGACCTACGGCGACGTATAGTCGAAGTGTTGTTATTGTTGATGACATAAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTAcc       >  2:801565/1‑141 (MQ=255)
                                                                                                                                  tttAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCa      >  2:785403/1‑141 (MQ=255)
                                                                                                                                    tAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAAc    <  2:285678/141‑1 (MQ=255)
                                                                                                                                      attattattTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAACGt  >  2:427851/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                       
AATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAACGT  >  CP000730/238034‑238308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: