Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 313,483 C→T 5.2% L220L (TTG→TTA USA300HOU_0279 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730313,4830CT 5.2% 376.0 / 3.4 120L220L (TTG→TTAUSA300HOU_0279hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (55/58);  new base T (5/2);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 122 total aligned reads displayed.

TCAAACATAGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAGC  >  CP000730/313343‑313619
                                                                                                                                            |                                                                                                                                        
 cAAACATAGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCa                                                                                                                                         <  1:100179/141‑1 (MQ=255)
         gATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCAttg                                                                                                                                 >  1:1073989/1‑141 (MQ=255)
         gATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCAttg                                                                                                                                 >  1:660102/1‑141 (MQ=255)
          aTACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCAttgt                                                                                                                                <  1:705141/141‑1 (MQ=255)
           tACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCAttgtt                                                                                                                               >  1:48263/1‑141 (MQ=255)
             cAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGa                                                                                                                             <  1:668860/141‑1 (MQ=255)
               aaGTACAGTAAGTGGCATTATATTTAGTACCCTTTTTTAGGGACAAAGGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATa                                                                                                                           >  2:38962/1‑141 (MQ=255)
                   aCAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCt                                                                                                                       <  2:767018/141‑1 (MQ=255)
                   aCAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCt                                                                                                                       >  1:125735/1‑141 (MQ=255)
                    cAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCtt                                                                                                                      >  2:907883/1‑141 (MQ=255)
                       ttAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTtat                                                                                                                   >  2:850155/1‑141 (MQ=255)
                       atAGTGGCTTTATATTTAGTGCTATTTTTTAGCGACAAATGTAATATAATTCATATCTTTACGGAATTTAGTCATCGTTTTAAACGTTTTCCAAAACATTGGACGGTTATCTTTATTCAAAGCATTGTTGATATTCTGtat                                                                                                                   >  2:302629/2‑141 (MQ=255)
                         aGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAg                                                                                                                 >  2:85640/1‑141 (MQ=255)
                           tGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGtt                                                                                                               <  1:1053820/141‑1 (MQ=255)
                           tGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGtt                                                                                                               >  1:447800/1‑141 (MQ=255)
                             gCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTcc                                                                                                             <  1:791131/141‑1 (MQ=255)
                                ttATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCcaac                                                                                                          >  2:984618/1‑141 (MQ=255)
                                ttATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCcaac                                                                                                          <  2:201660/141‑1 (MQ=255)
                                     tttAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATAc                                                                                                     <  1:1081736/141‑1 (MQ=255)
                                      ttAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATAcc                                                                                                    >  1:1042403/1‑141 (MQ=255)
                                           gCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGt                                                                                               <  1:641321/141‑1 (MQ=255)
                                            ctctTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGCTAATCTTTATAGTTCCAACAATACCTTCGtt                                                                                              >  2:870973/1‑140 (MQ=255)
                                             tctTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCa                                                                                             >  1:461360/1‑141 (MQ=255)
                                              ctTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCAt                                                                                            >  1:536877/1‑141 (MQ=255)
                                                    tAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTa                                                                                      <  2:792257/141‑1 (MQ=255)
                                                     aGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTaa                                                                                     >  2:671983/1‑141 (MQ=255)
                                                      gCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTaaa                                                                                    >  1:584170/1‑141 (MQ=255)
                                                      gCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTaaa                                                                                    <  1:761099/141‑1 (MQ=255)
                                                       cGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAAc                                                                                   <  1:1040408/141‑1 (MQ=255)
                                                        gACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAAcc                                                                                  >  2:386286/1‑141 (MQ=255)
                                                         aCAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCt                                                                                 <  1:56142/141‑1 (MQ=255)
                                                           aaaaGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTTAAAGCATTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCttt                                                                               <  2:414273/141‑1 (MQ=255)
                                                           aaaaGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCttt                                                                               <  1:741792/141‑1 (MQ=255)
                                                             aaGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTg                                                                             >  1:171088/1‑141 (MQ=255)
                                                             aaGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTg                                                                             <  1:895168/141‑1 (MQ=255)
                                                               gTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGgt                                                                           <  2:587678/141‑1 (MQ=255)
                                                                tAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGgtg                                                                          >  1:232628/1‑141 (MQ=255)
                                                                tAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACATTCGTCATAAATTAAACCTTTTGgtg                                                                          >  1:895799/1‑141 (MQ=255)
                                                                   tataATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCa                                                                       <  2:263558/141‑1 (MQ=255)
                                                                     taATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCTAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCAtt                                                                     <  2:934282/141‑1 (MQ=255)
                                                                     taATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAAt                                                                                        <  2:728993/122‑1 (MQ=255)
                                                                     taATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAAt                                                                                        >  1:728993/1‑122 (MQ=255)
                                                                     taATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCAtt                                                                     <  2:888776/141‑1 (MQ=255)
                                                                         tCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAAc                                                                 <  1:258422/141‑1 (MQ=255)
                                                                         tCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAAc                                                                 <  2:338335/141‑1 (MQ=255)
                                                                            tatCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCa                                                              >  2:59574/1‑141 (MQ=255)
                                                                            tatCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATGGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAAGTAAACCTTTTGGTGTCATNAAACTCa                                                              >  2:812840/1‑141 (MQ=255)
                                                                             atCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCAt                                                             <  2:1030455/141‑1 (MQ=255)
                                                                               cTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTg                                                           >  2:75298/1‑141 (MQ=255)
                                                                                   aCGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGACGATTTTCTTTATTCAAAGCATTGTTAATAATCTTTATAGTTCCAGCAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGAcc                                                       <  1:213486/141‑1 (MQ=255)
                                                                                    cGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCa                                                      <  2:867502/141‑1 (MQ=255)
                                                                                    cGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCa                                                      >  2:255232/1‑141 (MQ=255)
                                                                                     gCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAg                                                     <  2:343521/141‑1 (MQ=255)
                                                                                      cAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGt                                                    <  2:461978/141‑1 (MQ=255)
                                                                                         tttAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATg                                                 >  1:371100/1‑141 (MQ=255)
                                                                                           tAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGTACCAGTATGAt                                               >  1:459904/1‑141 (MQ=255)
                                                                                             gTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATaa                                             <  1:969801/141‑1 (MQ=255)
                                                                                              tCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTTTTTTCATTTTTAATAATCTTTATACTTCCAACAATACCTTCGTCACAAATGAAACCAGCTGGTGTCATTAAACTCATTGGACCAGTATGATAAt                                            >  2:620920/1‑141 (MQ=255)
                                                                                              tCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAAt                                            >  1:587662/1‑141 (MQ=255)
                                                                                               cATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATg                                           <  1:1064216/141‑1 (MQ=255)
                                                                                                aTCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTTAAAGCATTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGc                                          >  2:601813/1‑141 (MQ=255)
                                                                                                     tttAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATg                                     >  2:428097/1‑141 (MQ=255)
                                                                                                      ttAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTTTTTTTTTTTTTAATAATCTTTATAGTTCCAACATTACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATTCACATGa                                    >  2:203123/1‑141 (MQ=255)
                                                                                                      ttAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGa                                    <  2:970051/141‑1 (MQ=255)
                                                                                                      ttAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGa                                    <  1:40885/141‑1 (MQ=255)
                                                                                                       tAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGAt                                   <  1:484588/141‑1 (MQ=255)
                                                                                                       tAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGAt                                   <  1:694518/141‑1 (MQ=255)
                                                                                                         aaCATTTTACAAAACATTGGTAGACTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTa                                 >  2:545310/1‑141 (MQ=255)
                                                                                                          aCATTTTACAAAACATTGGTCGATTTTCTTTTTTTTTTTTTTTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTaa                                >  2:484013/1‑141 (MQ=255)
                                                                                                           cATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTaaa                               >  1:275977/1‑141 (MQ=255)
                                                                                                            aTTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTaaaa                              >  1:140836/1‑141 (MQ=255)
                                                                                                             ttttACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAAc                             >  2:276663/1‑141 (MQ=255)
                                                                                                             ttttACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTATCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAAc                             >  2:505114/1‑141 (MQ=255)
                                                                                                              tttACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAAcc                            <  1:729400/141‑1 (MQ=255)
                                                                                                              tttACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAAcc                            <  1:298133/141‑1 (MQ=255)
                                                                                                                tACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAg                          >  1:167851/1‑141 (MQ=255)
                                                                                                                tACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAg                          >  1:528520/1‑141 (MQ=255)
                                                                                                                 aCAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGc                         >  2:715466/1‑141 (MQ=255)
                                                                                                                    aaaCATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTg                      <  2:1078136/141‑1 (MQ=255)
                                                                                                                     aaCATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGa                     <  1:85640/141‑1 (MQ=255)
                                                                                                                     aaCATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGa                     >  1:343807/1‑141 (MQ=255)
                                                                                                                      aCATTGGTCGGTTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGAt                    <  1:38278/141‑1 (MQ=255)
                                                                                                                        aTTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCGACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATta                  >  1:185292/1‑141 (MQ=255)
                                                                                                                         ttGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtat                 >  1:520027/1‑141 (MQ=255)
                                                                                                                          ttgTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtata                <  2:80483/139‑1 (MQ=255)
                                                                                                                          tGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtata                >  1:848720/1‑141 (MQ=255)
                                                                                                                           ggTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtatat               <  2:499500/141‑1 (MQ=255)
                                                                                                                            gTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtatata              >  1:810296/1‑141 (MQ=255)
                                                                                                                             tCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATaa             <  2:1018038/141‑1 (MQ=255)
                                                                                                                              cGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATaaa            <  1:770845/141‑1 (MQ=255)
                                                                                                                                aTTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTCTGATCATGAACTTGCTGAAAACCAGCTTGATTATATAAATc          <  2:48263/141‑1 (MQ=255)
                                                                                                                                 ttttCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCt         >  1:1047417/1‑141 (MQ=255)
                                                                                                                                     cTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAAcc     <  1:65523/141‑1 (MQ=255)
                                                                                                                                     cTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAAcc     <  2:787969/141‑1 (MQ=255)
                                                                                                                                     cTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAAcc     <  1:598591/141‑1 (MQ=255)
                                                                                                                                       tttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAg   >  1:581756/1‑141 (MQ=255)
                                                                                                                                       tttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAg   <  2:347638/141‑1 (MQ=255)
                                                                                                                                       tttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAg   >  1:588603/1‑141 (MQ=255)
                                                                                                                                       tttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAg   <  2:451686/141‑1 (MQ=255)
                                                                                                                                        ttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAGc  >  1:17687/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                        
TCAAACATAGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAGC  >  CP000730/313343‑313619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: