Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 340,248 T→C 6.2% N1462N (AAT→AAC essC → virulence protein EssC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730340,2480TC 6.2% 279.8 / 6.5 96N1462N (AAT→AACessCvirulence protein EssC
Reads supporting (aligned to +/- strand):  ref base T (41/49);  new base C (3/3);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GAATTAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAA  >  CP000730/340111‑340387
                                                                                                                                         |                                                                                                                                           
gAATTAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGaa                                                                                                                                          <  2:71/141‑1 (MQ=255)
gAATTAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGaa                                                                                                                                          >  2:852379/1‑141 (MQ=255)
   ttAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATCATAGAAAATGAAGCa                                                                                                                                       <  1:1071533/141‑1 (MQ=255)
    tAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCat                                                                                                                                      >  1:69193/1‑141 (MQ=255)
       tataTGCTTATGATAAACAGATTGATGGTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCatata                                                                                                                                   <  2:53750/138‑1 (MQ=255)
          aTGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATgg                                                                                                                                <  2:493000/141‑1 (MQ=255)
          aTGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAAAAATTCTTTAAATTTATATTTATTCAACGAGAAAAAAAAAAAAAAAAAAATGAAGCATATATgg                                                                                                                                <  2:842311/141‑1 (MQ=255)
            gCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTc                                                                                                                              <  2:834402/141‑1 (MQ=255)
               tATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCa                                                                                                                           >  2:656304/1‑141 (MQ=255)
                 tGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCaaa                                                                                                                         >  2:293261/1‑141 (MQ=255)
                     aaaCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCaa                                                                                                                     >  2:934604/1‑141 (MQ=255)
                       aCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGc                                                                                                                   <  1:681864/141‑1 (MQ=255)
                       aCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGc                                                                                                                   <  1:604849/141‑1 (MQ=255)
                         aGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCtt                                                                                                                 <  1:885382/141‑1 (MQ=255)
                          gATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTa                                                                                                                >  1:346508/1‑141 (MQ=255)
                            ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGTCCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                              <  1:646306/141‑1 (MQ=255)
                            ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                              <  1:759011/141‑1 (MQ=255)
                            ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                              >  1:330797/1‑141 (MQ=255)
                             tGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCa                                                                                                             >  2:1050256/1‑141 (MQ=255)
                              gATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCaa                                                                                                            <  1:1048936/141‑1 (MQ=255)
                                 tttGCACNTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTCAAATTTAGATTAATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATAGTCGCAAACCAAGCTTATCAAAAg                                                                                                         <  2:814439/140‑1 (MQ=255)
                                 gTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAg                                                                                                         <  2:630391/141‑1 (MQ=255)
                                    gCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGAtt                                                                                                      <  2:172934/141‑1 (MQ=255)
                                    gCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGAtt                                                                                                      <  2:1037444/141‑1 (MQ=255)
                                       cGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGa                                                                                                   <  1:532827/141‑1 (MQ=255)
                                           aaaTGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGt                                                                                               >  2:1012616/1‑141 (MQ=255)
                                           aaaTGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGt                                                                                               <  2:629606/141‑1 (MQ=255)
                                               gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                           >  2:120142/1‑141 (MQ=255)
                                               gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                           <  2:781604/141‑1 (MQ=255)
                                               gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                           >  1:585893/1‑141 (MQ=255)
                                                aTTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaaa                                                                                          >  1:189720/1‑141 (MQ=255)
                                                 ttAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAAt                                                                                         <  2:745538/141‑1 (MQ=255)
                                                 ttAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAAt                                                                                         <  2:332311/141‑1 (MQ=255)
                                                   aaCCAATTTAGTATATGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCTTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAg                                                                                       <  1:411277/141‑1 (MQ=255)
                                                   aaCCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAg                                                                                       <  1:411365/141‑1 (MQ=255)
                                                      cAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCaa                                                                                    >  1:148899/1‑141 (MQ=255)
                                                        aTTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATg                                                                                  <  2:413284/141‑1 (MQ=255)
                                                         tttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGa                                                                                 >  1:435798/1‑141 (MQ=255)
                                                          ttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGaa                                                                                >  2:866594/1‑141 (MQ=255)
                                                          ttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGaa                                                                                >  2:32314/1‑141 (MQ=255)
                                                            aGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAAtt                                                                              <  2:31376/141‑1 (MQ=255)
                                                            aGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAAtt                                                                              <  2:31327/141‑1 (MQ=255)
                                                                taGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAAt                                                                          <  2:504951/141‑1 (MQ=255)
                                                                  ggTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATag                                                                        <  2:23317/141‑1 (MQ=255)
                                                                     aTTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATaggag                                                                     >  2:239750/1‑141 (MQ=255)
                                                                       tCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggg                                                                   <  2:124162/141‑1 (MQ=255)
                                                                       tCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggg                                                                   >  2:426875/1‑141 (MQ=255)
                                                                        cGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggga                                                                  <  2:614027/141‑1 (MQ=255)
                                                                         gTATTTCAGACCACCAATTCTTTCAATTTAGATTTATTCAACGGCAAGCTTTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggagggag                                                                 <  1:585598/141‑1 (MQ=255)
                                                                         gTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAAACAAGATTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggagggag                                                                 >  1:701982/1‑141 (MQ=255)
                                                                            tttCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTa                                                              <  1:215862/141‑1 (MQ=255)
                                                                            tttCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTa                                                              <  1:215900/141‑1 (MQ=255)
                                                                              tCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATg                                                            <  2:336514/141‑1 (MQ=255)
                                                                              tCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATg                                                            <  2:502518/141‑1 (MQ=255)
                                                                                  aCCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTGAATAGGAGGGAGGTATGTTAt                                                        >  2:918011/1‑141 (MQ=255)
                                                                                   ccAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATg                                                       >  1:492990/1‑141 (MQ=255)
                                                                                        aaTTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAAttt                                                  >  1:1008493/1‑141 (MQ=255)
                                                                                          ttCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTa                                                <  2:726202/141‑1 (MQ=255)
                                                                                           tCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTaa                                               >  1:811610/1‑141 (MQ=255)
                                                                                            cTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAAt                                              <  1:823348/141‑1 (MQ=255)
                                                                                              ttAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGa                                            >  2:924533/1‑141 (MQ=255)
                                                                                              ttAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGa                                            >  2:890172/1‑141 (MQ=255)
                                                                                               tAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGat                                           >  1:645917/1‑141 (MQ=255)
                                                                                                     tAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTACATAGGCGGGAGGTATGTTATGAATTTTAATGATATTGaa                                     <  2:772061/141‑1 (MQ=255)
                                                                                                      aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                    >  1:82643/1‑141 (MQ=255)
                                                                                                      aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                    >  2:911368/1‑141 (MQ=255)
                                                                                                      aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                    >  2:1029942/1‑141 (MQ=255)
                                                                                                      aGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                    >  2:139775/1‑141 (MQ=255)
                                                                                                      aGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                    <  2:278669/141‑1 (MQ=255)
                                                                                                       gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAAc                                   <  2:138364/141‑1 (MQ=255)
                                                                                                       gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAAc                                   <  1:872065/141‑1 (MQ=255)
                                                                                                       gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGCATGTTATGAATTTTAATGATATTGAAAc                                   >  2:983063/1‑141 (MQ=255)
                                                                                                               cAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTa                           <  2:196713/141‑1 (MQ=255)
                                                                                                               cAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGCTATGTTATGAATTTTAATGATATTGAAACAATGGTTa                           <  1:785049/141‑1 (MQ=255)
                                                                                                                 aCGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAg                         >  1:862912/1‑141 (MQ=255)
                                                                                                                 aCGAGAACCTGTTATTAAAGAAAATGAAGCATACACGGTCGCAAACCAAGATTCTCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAg                         >  2:593635/1‑141 (MQ=255)
                                                                                                                   gagaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTc                       <  2:359229/141‑1 (MQ=255)
                                                                                                                   gagaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTc                       <  1:41422/141‑1 (MQ=255)
                                                                                                                    agaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCg                      <  1:622868/141‑1 (MQ=255)
                                                                                                                    agaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCg                      <  2:527587/141‑1 (MQ=255)
                                                                                                                     gaACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAGTTTTAATGATATTGAAACAATGGTCAAGTCGa                     >  1:930400/1‑141 (MQ=255)
                                                                                                                     gaACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTCAAGTCGa                     >  1:930450/1‑141 (MQ=255)
                                                                                                                       aCCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGaaa                   >  2:132203/1‑141 (MQ=255)
                                                                                                                       aCCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGaaa                   >  1:61843/1‑141 (MQ=255)
                                                                                                                        ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                  >  2:673993/1‑141 (MQ=255)
                                                                                                                        ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                  >  2:482835/1‑141 (MQ=255)
                                                                                                                        ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                  >  2:531337/1‑141 (MQ=255)
                                                                                                                         cTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAtt                 >  2:161961/1‑141 (MQ=255)
                                                                                                                         cTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAtt                 >  1:430977/1‑141 (MQ=255)
                                                                                                                                 aaaGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGata         <  1:167206/141‑1 (MQ=255)
                                                                                                                                   aGAAAATGAAGCATATATGGTCGCAAACCAAGATTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATAtt       >  1:575556/1‑141 (MQ=255)
                                                                                                                                     aaaaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaa     <  1:215270/141‑1 (MQ=255)
                                                                                                                                     aaaaTAATGTATATATTTTCGCGAACAAAGCTGATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATAATATTGAAACAATGTTTAAGTCGAAATTTAAAGATATTaa     <  2:464900/141‑1 (MQ=255)
                                                                                                                                      aaaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaa    <  2:979141/141‑1 (MQ=255)
                                                                                                                                       aaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaaa   >  2:451349/1‑141 (MQ=255)
                                                                                                                                        aTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaaat  <  1:1029711/141‑2 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GAATTAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAA  >  CP000730/340111‑340387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: