BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,015	C→T	24.3%	R179R (AGG→AGA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,015	0	C	T	24.3%	524.4 / 174.0	300	R179R (AGG→AGA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (99/127); new base T (33/40); total (133/167)
Fisher's exact test for biased strand distribution p-value = 8.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.01e-01

Only 100 of 307 total aligned reads displayed.

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                                              <  2:142546/139‑1 (MQ=17)

gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                                              <  2:296195/139‑1 (MQ=17)

 cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                                             <  1:217000/141‑1 (MQ=17)

  ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                                            <  1:297079/141‑1 (MQ=17)

  ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                                            <  2:415735/141‑1 (MQ=17)

    aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                                                                                                          <  2:163176/141‑1 (MQ=11)

    aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                                                                                                          <  1:191713/141‑1 (MQ=11)

      cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                                        >  1:94391/1‑141 (MQ=32)

      cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                                        >  1:382687/1‑141 (MQ=17)

      cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                                        <  2:342973/141‑1 (MQ=17)

       ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                                       <  1:98468/141‑1 (MQ=32)

        tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                                      <  1:452416/141‑1 (MQ=17)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                     >  2:451121/1‑141 (MQ=17)

               atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                                                                                               >  1:113635/1‑141 (MQ=33)

                    gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                                                                          <  2:55767/141‑1 (MQ=25)

                        aTTTCGTGGGATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGTGCGTAAATACACTCATTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatgtat                                                                                                                      <  2:413076/141‑5 (MQ=11)

                        aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAATTTGATACCTTGTCTTTCTTACTTTAATATGa                                                                                                                      <  2:251401/141‑1 (MQ=12)

                        aTTTCGTAGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                                                                                                                      >  1:401850/1‑141 (MQ=25)

                            cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                                  <  1:263743/141‑1 (MQ=14)

                            cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATGCACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                                  >  2:344162/1‑141 (MQ=255)

                            cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                                  <  1:242218/141‑1 (MQ=38)

                            cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                                  <  2:195661/141‑1 (MQ=38)

                             gTGGCGTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATATAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTTCTTTAATATGACGGTg                                                                                                                 >  2:104097/1‑141 (MQ=11)

                             gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 >  1:165686/1‑141 (MQ=255)

                               ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                                                                               >  1:201518/1‑141 (MQ=14)

                               ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                                                                               >  1:105929/1‑141 (MQ=255)

                                gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                                              >  2:144197/1‑141 (MQ=14)

                                  gtGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTGTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTGTTACTTTAATATGACGGTGATCtt                                                                                                            <  2:418248/140‑1 (MQ=255)

                                      cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                                         >  2:218087/1‑141 (MQ=255)

                                         aaaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     >  1:338546/1‑141 (MQ=37)

                                         aaaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     <  1:264873/141‑1 (MQ=255)

                                         aaaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     >  1:304464/1‑141 (MQ=37)

                                          aaTCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGGGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                                                                    <  2:425333/141‑1 (MQ=255)

                                           aTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                   <  1:284159/141‑1 (MQ=255)

                                           aTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAACTGGTCTTTCTTACTTTAATATGACGGTGGCCTTGCTCAATGa                                                                                                   >  1:347481/1‑141 (MQ=11)

                                              cGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGACACTTTGATATCTTGTCTTTCTTACTTTAATATGTCGGTGATCTTGCTCAATGAGGt                                                                                                <  2:446527/141‑1 (MQ=14)

                                                tAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                                              >  1:232537/1‑141 (MQ=255)

                                                tAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                                              >  2:293689/1‑141 (MQ=37)

                                                tAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                                              >  1:100003/1‑141 (MQ=255)

                                                  gATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                                            <  2:319915/141‑1 (MQ=255)

                                                  gATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                                            >  2:274651/1‑141 (MQ=255)

                                                      tGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACGTTAATATGACGGTGTTCTTGCTCAATGAGGTTATTCAGa                                                                                        <  2:40343/141‑1 (MQ=255)

                                                         agagACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat                                                                                     <  1:311704/141‑1 (MQ=255)

                                                             aCCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                                                 >  2:199997/1‑141 (MQ=39)

                                                              ccTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                                                >  1:447716/1‑141 (MQ=255)

                                                                tGCGGTTC‑TTTTTATCTAGAGCGTAAATAAACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTAGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCACTGAGGTTATTAACATATTTCGAta                                                                              >  2:40840/1‑140 (MQ=14)

                                                                 gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                             <  2:35364/141‑1 (MQ=255)

                                                                    gTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                                                                          >  2:174129/1‑141 (MQ=39)

                                                                         tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                                      <  2:73885/141‑1 (MQ=40)

                                                                         tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                                      >  1:434073/1‑141 (MQ=255)

                                                                           tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACa                                                                    <  1:126533/141‑1 (MQ=255)

                                                                             tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                  >  2:370401/1‑141 (MQ=255)

                                                                                ataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCCTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAg                                                               <  1:372215/141‑1 (MQ=255)

                                                                                 taGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                                              <  2:230361/141‑1 (MQ=255)

                                                                                 taGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                                              <  1:368992/141‑1 (MQ=255)

                                                                                   gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                                            <  2:26485/141‑1 (MQ=255)

                                                                                     gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttta                                                          <  1:276421/141‑1 (MQ=255)

                                                                                     gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                                          <  2:245563/141‑1 (MQ=255)

                                                                                          aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgattaagttt                                                     >  2:365608/1‑141 (MQ=255)

                                                                                            tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                   <  1:135256/141‑1 (MQ=255)

                                                                                               actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTCATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAg                                                <  2:436896/139‑1 (MQ=255)

                                                                                                ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                                               <  1:1546/140‑1 (MQ=255)

                                                                                                ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                                               >  1:387558/2‑141 (MQ=255)

                                                                                                 tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                                              <  2:138534/141‑1 (MQ=255)

                                                                                                 tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                                              <  1:424863/141‑1 (MQ=255)

                                                                                                  cAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCtt                                             >  2:159073/1‑141 (MQ=255)

                                                                                                    ttaacTTTGAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                                           <  2:128245/137‑1 (MQ=255)

                                                                                                    aTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                                           <  1:255234/141‑1 (MQ=255)

                                                                                                        cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                       >  2:23597/1‑141 (MQ=255)

                                                                                                         ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGCTTTAAGTTTAAAAGCTTTAATTAc                                      >  2:428937/1‑141 (MQ=255)

                                                                                                         ttttAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTat                                      >  2:183874/1‑140 (MQ=255)

                                                                                                          tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACt                                     >  1:238620/1‑141 (MQ=255)

                                                                                                            tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                   >  1:66329/1‑141 (MQ=255)

                                                                                                            tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGCCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                   >  2:287363/1‑141 (MQ=255)

                                                                                                             aaaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTa                                  <  2:456899/141‑1 (MQ=255)

                                                                                                             aaaGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGCACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTa                                  >  2:134608/1‑141 (MQ=255)

                                                                                                              aaGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAg                                 >  2:187983/1‑141 (MQ=255)

                                                                                                                gTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTAAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAcc                               >  2:177722/1‑141 (MQ=255)

                                                                                                                 tATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCa                              >  2:148946/1‑141 (MQ=255)

                                                                                                                 tATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCa                              >  2:386716/1‑141 (MQ=40)

                                                                                                                 tATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCa                              <  1:177424/141‑1 (MQ=255)

                                                                                                                   ttCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt                            <  2:112753/141‑1 (MQ=255)

                                                                                                                       ttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtg                        >  1:112515/1‑140 (MQ=255)

                                                                                                                        tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAc                       >  2:377719/1‑141 (MQ=255)

                                                                                                                         gccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtgcc                      <  1:353663/138‑4 (MQ=255)

                                                                                                                         gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc                      <  1:321027/141‑1 (MQ=255)

                                                                                                                         gCTGGATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc                      <  1:227608/141‑1 (MQ=255)

                                                                                                                          cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                     <  1:39580/141‑1 (MQ=255)

                                                                                                                          cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                     >  1:212381/1‑141 (MQ=255)

                                                                                                                          cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                     >  1:187111/1‑141 (MQ=255)

                                                                                                                          cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                     >  2:309693/1‑141 (MQ=255)

                                                                                                                            gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTc                   >  1:307411/1‑141 (MQ=255)

                                                                                                                              aTTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTAGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGt                 >  2:434365/1‑141 (MQ=255)

                                                                                                                                   tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg            <  2:66329/141‑1 (MQ=255)

                                                                                                                                   tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg            <  2:50390/141‑1 (MQ=255)

                                                                                                                                     cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGt          <  1:77593/141‑1 (MQ=255)

                                                                                                                                        tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                   >  2:270479/1‑113 (MQ=40)

                                                                                                                                           tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa    >  2:82550/1‑141 (MQ=255)

                                                                                                                                           tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGa    >  1:22359/1‑141 (MQ=38)

                                                                                                                                             ccTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGCAGGTGCCTGATc  >  2:397808/1‑141 (MQ=255)

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`