Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,070,854 T→C 7.7% intergenic (‑20/‑250) ykgB ← / → ykgB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,070,8540TC 7.7% 140.1 / 3.1 52intergenic (‑20/‑250)ykgB/ykgBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/30);  new base C (2/2);  total (20/32)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

TTCTAATTCAAATCCTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCGATTTTAATTAATCCCTCTTT  >  CP000730/2070718‑2070992
                                                                                                                                        |                                                                                                                                          
ttCTAATTCAAATCCTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATATTAGtt                                                                                                                                        >  1:408011/1‑140 (MQ=255)
  cTAATTCAAATCCTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGCAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCtt                                                                                                                                      <  1:349732/141‑1 (MQ=255)
   tAATTCAAATCCTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTa                                                                                                                                     >  2:382039/1‑141 (MQ=255)
   tAATTCAAATCCTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTa                                                                                                                                     <  1:366223/141‑1 (MQ=255)
       tCAAATCCTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTAtttt                                                                                                                                 >  1:150485/1‑141 (MQ=255)
             ccTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCAtttt                                                                                                                           >  1:196208/1‑141 (MQ=255)
              cTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTtta                                                                                                                          <  2:275172/141‑1 (MQ=255)
               tGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTttat                                                                                                                         <  2:453647/141‑1 (MQ=255)
                gTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTttatt                                                                                                                        >  2:451004/1‑141 (MQ=255)
                     caataaATCACTACGTGACTGATATTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAAGATATCCATTCGTCATTACTTTTACTACTCCATCTTCGCCTTATCTTTATTTTGTTACAtt                                                                                                                   >  2:371725/2‑141 (MQ=255)
                       ataaATCCATACGTGACTGATTTTCGTTTAATTCATAACGATAAATCCCTTTACCATTCTTTTTAGTGTACGAACCAATATATCCATTTGTCATTACTTTTACCCCACCATGTTAGTCTTATTTTCATTTTATTAAAtttt                                                                                                                 <  2:263431/141‑1 (MQ=255)
                       ataaATCAATACGTGACTGATTTTCGTTCAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATCTATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTACTTTCATTTTATTAAAtctt                                                                                                                 <  2:386543/141‑1 (MQ=255)
                        taaATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTa                                                                                                                >  1:76856/1‑141 (MQ=255)
                                tACGTGTCTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAAt                                                                                                        <  2:370147/141‑1 (MQ=255)
                                  cGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTa                                                                                                      >  1:59103/1‑141 (MQ=255)
                                    tcaataaTTTTCTTTTAATTCAAATCTATAATTTCCTTTAACATTCTTTTTTTTATAATAACTAATTTATCCTTTTTTTATAACTTTTATCTTTCCATATTAATCTTATTTTCATTTTATTAATTTTTAGCTATCATTAAc                                                                                                    <  2:295620/135‑1 (MQ=255)
                                    tGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAAc                                                                                                    <  2:295615/141‑1 (MQ=255)
                                       cTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAat                                                                                                 >  1:104510/1‑141 (MQ=255)
                                         gATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAatca                                                                                               <  2:282690/141‑1 (MQ=255)
                                             tttttttttttttaaaCGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTCAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAatcattta                                                                                           >  2:124002/14‑141 (MQ=255)
                                              tCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCAGGTCAGTCTTATTTTCANTTTATTAAATTTTAGCTAGAATTAACAatcatttat                                                                                          <  1:95506/141‑1 (MQ=255)
                                               cGTTTAATTCAAAACGATAAATCCCTTTATCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAatcatttatc                                                                                         <  2:33951/141‑1 (MQ=255)
                                                gTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAatcatttatca                                                                                        <  2:319168/141‑1 (MQ=255)
                                                gTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAatcatttatca                                                                                        <  2:195388/141‑1 (MQ=255)
                                                 tttAACTCAAAACGATAAATGCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAatcatttatcat                                                                                       <  2:52354/141‑1 (MQ=255)
                                                   tAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAatcatttatcattt                                                                                     <  2:297087/141‑1 (MQ=255)
                                                    aaTTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTc                                                                                    <  1:244660/141‑1 (MQ=255)
                                                    aaTTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTc                                                                                    <  1:244659/141‑1 (MQ=255)
                                                     aTTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTcc                                                                                   >  2:361254/1‑141 (MQ=255)
                                                           aaCGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAg                                                                             <  2:155029/141‑1 (MQ=255)
                                                                  aaTCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAAc                                                                      >  2:166067/1‑141 (MQ=255)
                                                                  aaTCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTGACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAAc                                                                      >  1:447045/1‑141 (MQ=255)
                                                                          tACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAac                                                              <  2:69489/141‑1 (MQ=255)
                                                                           aCCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAact                                                             <  1:148050/141‑1 (MQ=255)
                                                                             cATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTCAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAACGCTAAAACTGTTTAactac                                                           <  1:344298/141‑1 (MQ=255)
                                                                              aTTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAactact                                                          <  2:114170/141‑1 (MQ=255)
                                                                                   ttttAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTaaa                                                     <  1:400878/141‑1 (MQ=255)
                                                                                     ttAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAg                                                   >  1:382394/1‑141 (MQ=255)
                                                                                         tgtAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACt                                               <  1:425222/141‑1 (MQ=255)
                                                                                           tAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTACATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTAt                                             <  2:408011/141‑1 (MQ=255)
                                                                                             aGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTAttt                                           <  2:56060/141‑1 (MQ=255)
                                                                                                  cAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCtgt                                      >  2:143280/1‑141 (MQ=255)
                                                                                                                 tCATTACTTTTGCCCCTCCATGTTAGTCTTATTTTCACTTTATTAACTTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAGAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTc                       <  2:446401/141‑1 (MQ=255)
                                                                                                                 tCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTc                       <  2:122536/141‑1 (MQ=255)
                                                                                                                 tCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTc                       <  1:62011/141‑1 (MQ=255)
                                                                                                                  cATTACTTTTACCCCTCCATGTCAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAACGCTAAAACTGTTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCg                      >  1:199255/1‑141 (MQ=255)
                                                                                                                   aTTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCGa                     <  1:239429/141‑1 (MQ=255)
                                                                                                                    ttACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCGAt                    >  1:250172/1‑141 (MQ=255)
                                                                                                                          ttACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCGATTttaat              >  1:134545/1‑141 (MQ=255)
                                                                                                                             ccccTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCGATTttaattaa           <  1:319856/141‑1 (MQ=255)
                                                                                                                                 tCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCGATTTTAATTAATccc       >  1:402466/1‑141 (MQ=255)
                                                                                                                                      gTTAGTCTTATTTTCATTTTATTAAATTTTGGCTAGAATTGACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCGATTTTAATTAATCCCTCttt  >  2:363447/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TTCTAATTCAAATCCTGTTTCTAATAAATCAATACGTGACTGATTTTCGTTTAATTCAAAACGATAAATCCCTTTACCATTCTTTTTAGTGTAAGAACCAATATATCCATTTGTCATTACTTTTACCCCTCCATGTTAGTCTTATTTTCATTTTATTAAATTTTAGCTAGAATTAACAATCATTTATCATTTCCTTAAAGCTAAAACTATTTAACTACTTTAAAAGAACTATTTGCTGTGATATGTCATTATTCGATTTTAATTAATCCCTCTTT  >  CP000730/2070718‑2070992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑