BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,161	T→C	24.1%	I131V (ATT→GTT)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,161	0	T	C	24.1%	291.3 / 126.5	213	I131V (ATT→GTT)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (83/78); new base C (25/26); total (109/104)
Fisher's exact test for biased strand distribution p-value = 8.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 220 total aligned reads displayed.

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

 tttcttACTTGAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAtt                                                                                                                                              <  1:181552/141‑1 (MQ=255)

  ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTa                                                                                                                                             <  1:179274/141‑1 (MQ=255)

    cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                                                                                                           >  1:374152/1‑141 (MQ=255)

       aCTTTAATATGATGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTGGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCttt                                                                                                                                        <  2:366876/141‑1 (MQ=11)

            aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGatg                                                                                                                                   <  1:124277/141‑3 (MQ=255)

             atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGt                                                                                                                                  >  1:107197/1‑141 (MQ=255)

             atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGt                                                                                                                                  <  2:419409/141‑1 (MQ=35)

             atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGt                                                                                                                                  <  2:104203/141‑1 (MQ=35)

              tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                                 >  1:294001/1‑141 (MQ=25)

              tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                                 <  2:239481/141‑1 (MQ=255)

              tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                                 >  1:136073/1‑141 (MQ=255)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                              <  1:128436/141‑1 (MQ=21)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                             >  1:76773/1‑141 (MQ=255)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                             >  2:402498/1‑141 (MQ=255)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAcc                                                                                                                             <  2:227080/141‑1 (MQ=11)

                    ggTGATCTTGCTCAATGTAGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACTTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                           <  2:304464/141‑1 (MQ=11)

                    ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                           <  2:256666/141‑1 (MQ=18)

                    ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                           >  1:418584/1‑141 (MQ=16)

                     gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                          <  2:262874/141‑1 (MQ=12)

                     gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                          <  2:337801/141‑1 (MQ=11)

                      tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                                                                                                         <  1:100437/141‑1 (MQ=12)

                       gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                        <  2:447716/141‑1 (MQ=17)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGCAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                      >  2:123805/1‑141 (MQ=12)

                           ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGttt                                                                                                                    >  1:274436/1‑141 (MQ=32)

                             gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                  <  1:54491/141‑1 (MQ=12)

                              cTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                 <  2:244825/141‑1 (MQ=17)

                               tCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATg                                                                                                                >  2:141207/1‑141 (MQ=11)

                                cAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                               <  2:175331/141‑1 (MQ=12)

                                cAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                               <  2:301100/141‑1 (MQ=17)

                                  aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTTAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgaga                                                                                                             >  1:301737/1‑141 (MQ=255)

                                  aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgaga                                                                                                             >  1:408340/1‑141 (MQ=17)

                                  aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgaga                                                                                                             >  2:373590/1‑141 (MQ=12)

                                   tGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTAAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                                                                                                 >  1:316619/1‑118 (MQ=11)

                                    gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                                                                                                           >  1:341440/1‑141 (MQ=12)

                                      ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                         >  1:33662/1‑141 (MQ=12)

                                      ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                         >  2:331924/1‑141 (MQ=12)

                                      ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                         >  2:205707/1‑141 (MQ=12)

                                      ggTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                         >  2:360192/1‑141 (MQ=32)

                                        ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                                                                                                       >  2:146360/1‑141 (MQ=32)

                                         tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                      <  1:36008/141‑1 (MQ=17)

                                          aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                     >  1:230771/1‑141 (MQ=12)

                                            tCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTGCTTTGTTTTGATGGGCCGGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                                                                                                   >  1:201175/1‑141 (MQ=11)

                                             cAGATATTTCGGTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                  >  2:74281/1‑141 (MQ=255)

                                               gATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                                >  2:370044/1‑141 (MQ=11)

                                                 tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                              >  1:27799/1‑141 (MQ=12)

                                                 tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                              >  1:180199/1‑141 (MQ=12)

                                                 tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGTTGCCTAATCTGTAATTACTTTTTGAGGTTTACTAAATTGTTCAATGAGACGTTTGATAAACGcc                                                                                              >  1:158970/1‑140 (MQ=11)

                                                 tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                              <  1:251989/141‑1 (MQ=32)

                                                  atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                             <  2:55745/141‑1 (MQ=11)

                                                  atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTGTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                             <  2:386287/141‑1 (MQ=11)

                                                   tttCGCTTTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGTTACCTTCGTTGAAGGTGACTGATCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTAATGATTCGTTTTTTAATCGCata                                                                                            >  2:48749/1‑141 (MQ=11)

                                                   tttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCata                                                                                            >  1:391763/1‑141 (MQ=17)

                                                   tttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCata                                                                                            >  1:259092/1‑141 (MQ=12)

                                                     tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                          <  2:346891/141‑1 (MQ=17)

                                                     tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                          >  1:228465/1‑141 (MQ=12)

                                                     tCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                          >  1:421200/1‑141 (MQ=32)

                                                         tGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTATCTTTTGAGGCTTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATACTGa                                                                                      >  2:334207/1‑141 (MQ=11)

                                                         tGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTTCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAAAGCATATGCtca                                                                                      >  2:179375/1‑139 (MQ=11)

                                                              aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                 <  2:289598/141‑1 (MQ=14)

                                                              aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                 <  1:89437/141‑1 (MQ=32)

                                                                 gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATgtgtat                                                                              >  2:439048/1‑136 (MQ=11)

                                                                                                   tttAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAatgtatg                                           <  1:174129/141‑1 (MQ=11)

                                                                                                   tttAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAatgtatg                                           >  2:66246/1‑141 (MQ=11)

                                                                                                    ttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAatgtatgt                                          >  2:329296/1‑141 (MQ=9)

                                                                                                    ttAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAatgtatgt                                          <  1:218087/141‑1 (MQ=31)

                                                                                                     taacgaTTGCTGCCTTCGTTGAAGTTGCCTGATCTGTAATTTCCTTTTGAGGTTTACCAAATTGTTTAATGGGACGTTTGTGAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTc                                         <  2:400322/136‑1 (MQ=11)

                                                                                                        ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCct                                      <  2:219918/141‑1 (MQ=32)

                                                                                                            tGCTACCTTCGTTGAAGGTGCCTGATCTTTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                  >  1:161146/1‑141 (MQ=17)

                                                                                                            tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGGTTATCTCGTTCC‑TTACGCACCCAAATATCTAATGTATGTTCctcagt                                  >  2:195578/1‑138 (MQ=11)

                                                                                                            tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTgt                                  >  1:62414/1‑140 (MQ=17)

                                                                                                             gCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                 <  2:110822/141‑3 (MQ=255)

                                                                                                             gCTACCTTCGTTGAGGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                 <  1:344162/141‑3 (MQ=17)

                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             >  1:349562/1‑141 (MQ=17)

                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                             <  1:452061/141‑1 (MQ=32)

                                                                                                                  cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                            >  1:142238/1‑141 (MQ=17)

                                                                                                                  cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                            >  1:108635/1‑141 (MQ=17)

                                                                                                                   ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                           <  1:253412/141‑1 (MQ=17)

                                                                                                                      gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGATTTAACAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAAAAACTAATGTATGTCCCTCTGCATCAATGGCa                        <  2:428006/141‑1 (MQ=255)

                                                                                                                      gTTGAAGGGGCCTGATCTGTAATTACCTTTTGACGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTAAGCAACCAAATATCTAATGTATGTCCCTCTCTATCAATGTCa                        >  2:315986/1‑141 (MQ=11)

                                                                                                                       ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAc                       >  2:187710/1‑141 (MQ=32)

                                                                                                                       ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAc                       <  1:208146/141‑1 (MQ=32)

                                                                                                                       ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAc                       >  1:399953/1‑141 (MQ=32)

                                                                                                                       ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                       <  1:382623/141‑1 (MQ=255)

                                                                                                                        tGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                      <  2:337501/141‑1 (MQ=32)

                                                                                                                         gAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGa                     <  2:267284/141‑1 (MQ=32)

                                                                                                                           aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGata                   <  1:440213/141‑1 (MQ=17)

                                                                                                                           aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGata                   >  1:304726/1‑141 (MQ=32)

                                                                                                                           aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGata                   >  1:419527/1‑141 (MQ=32)

                                                                                                                             gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCTCTCTGTATCAATGGCACGAtata                 >  1:3676/1‑141 (MQ=17)

                                                                                                                                ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAt              >  1:116300/1‑141 (MQ=32)

                                                                                                                                ccTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATTGCACGATATAAAt              >  2:280837/1‑141 (MQ=255)

                                                                                                                                  tGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTCC‑TTACGCAACCAAAGATCTAATGCATGTCCCTCTGCATCAATGCTACGATATTAATAg            >  2:86050/1‑141 (MQ=11)

                                                                                                                                  tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg            <  2:362926/141‑1 (MQ=255)

                                                                                                                                   gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc           >  1:450726/1‑141 (MQ=255)

                                                                                                                                     tCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc         <  2:70935/141‑1 (MQ=17)

                                                                                                                                      cTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc        <  1:378256/141‑1 (MQ=12)

                                                                                                                                       tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa       <  1:16890/141‑1 (MQ=32)

                                                                                                                                         tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt      >  1:414867/1‑141 (MQ=12)

                                                                                                                                           aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAtttt   >  2:378087/1‑141 (MQ=32)

                                                                                                                                            ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc  <  2:105929/141‑1 (MQ=17)

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGC‑TTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`