| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 942,216 | T→A | 9.7% | intergenic (‑187/+62) | USA300HOU_0905 ← / ← mnhG2 | hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 942,216 | 0 | T | A | 9.7% | 120.3 / 4.8 | 41 | intergenic (‑187/+62) | USA300HOU_0905/mnhG2 | hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G |
| Reads supporting (aligned to +/- strand): ref base T (13/24); new base A (2/2); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01 | |||||||||||
TTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTT > CP000730/942081‑942346 | ttatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAAttt < 1:345500/141‑1 (MQ=255) taataaATAACGACATGAAAGGAAATTAAATTAAAGAAATATATGTAGATAGGGCGAATATAAAAAAAAAAAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc < 2:220694/141‑1 (MQ=255) aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa < 1:215601/141‑1 (MQ=255) tAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAATGATTTTAc < 2:131491/141‑1 (MQ=38) aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg < 1:204046/141‑1 (MQ=255) aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg < 1:106191/141‑1 (MQ=255) cGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActgc < 1:37287/141‑1 (MQ=255) gACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAAGAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActgct < 2:447180/141‑1 (MQ=255) tGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGTATCATCTTACTGCtgttt < 1:171914/141‑1 (MQ=255) tGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgttt < 2:17175/141‑1 (MQ=255) aaaGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttgt < 1:445091/141‑1 (MQ=255) aataaaGAGGTATATGTAGGGAGGGCGAATCTGTAGTCAAAGAAAAGAACATTGAAAAAGAATTAACAATTTCAAAAGAAAGCAGCAGTCAAATAATTCCTTATTTGGAATCAGCTTACTGCTGTTTGTTGTTGAATtata < 2:215943/139‑1 (MQ=255) aaaGAGATATATGTAGATAGGGCGGATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTc > 1:362076/1‑141 (MQ=255) agagATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCAt < 1:232366/141‑1 (MQ=255) gaTATATGTAGATAAGGACAATCAGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAt < 2:18749/141‑1 (MQ=255) tatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTg < 1:203940/141‑1 (MQ=255) aagTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGt < 1:216705/139‑1 (MQ=255) ccgagaaaaaaacaaaaGAAAAAATCCTTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa < 2:220269/127‑1 (MQ=255) taaatAGTAAAAAAAAAAATCATAGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAtt < 2:65434/137‑1 (MQ=255) cTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAttt < 2:242593/141‑1 (MQ=255) gTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtg > 1:392516/1‑141 (MQ=255) tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt < 1:410490/141‑1 (MQ=255) aGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgtg > 1:322954/1‑141 (MQ=255) gTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTgtgt > 1:452388/1‑141 (MQ=255) gTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTgtgt > 1:312289/1‑141 (MQ=255) aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt > 2:359037/1‑141 (MQ=255) gAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAatgat < 2:162191/73‑4 (MQ=18) gAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAatgat > 1:162191/1‑70 (MQ=18) tCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTc < 2:21859/141‑1 (MQ=255) aagtGAAAATGAGGTAAGTATTTCAAGAGAAATCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTGTTTGATTTATATTCATGATTTTGTTATATAATCTACTATTTTGTGTCTTTTAAGTCTTcc < 2:108748/138‑1 (MQ=255) tGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGaa > 1:400330/1‑141 (MQ=255) agagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCa < 2:58002/141‑1 (MQ=255) ggTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCg < 2:52020/141‑1 (MQ=255) gTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGCATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGa > 1:374343/1‑141 (MQ=255) aaTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTACGAAATTTCATCGACTTTa > 2:53259/1‑141 (MQ=255) aTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAg > 2:340207/1‑141 (MQ=255) aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt > 2:108889/1‑141 (MQ=255) aaaaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATCACTGATTTAGTTATATAATCTACACCTTTGTGCCTTTTAAGTCTTCCGAAATTTCATAGCCTTTAGTCTTTTTAGt > 1:223167/1‑141 (MQ=255) agcagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATATTCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAg > 2:109974/1‑141 (MQ=255) cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGACATTTCATCGACTTTAGTCTTTTTAGTATAAGGc < 2:273109/141‑1 (MQ=255) agatgaTTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt > 2:266396/6‑141 (MQ=38) | TTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTT > CP000730/942081‑942346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |