| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,250,361 | C→T | 13.9% | A44V (GCA→GTA) | ftsY → | Sec family Type I general secretory pathway protein signal recognition particle protein FtsY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,250,361 | 0 | C | T | 13.9% | 82.7 / 5.2 | 36 | A44V (GCA→GTA) | ftsY | Sec family Type I general secretory pathway protein signal recognition particle protein FtsY |
| Reads supporting (aligned to +/- strand): ref base C (18/13); new base T (3/2); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTAAA > CP000730/1250222‑1250497 | ggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCa < 2:201906/141‑1 (MQ=255)ggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCa < 1:218103/141‑1 (MQ=255)ggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCa < 1:392303/141‑1 (MQ=255) gagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCaa > 2:287613/1‑141 (MQ=255) tGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAg > 2:243661/1‑141 (MQ=255) tGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAg < 1:344285/141‑1 (MQ=255) gCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTATGAAGGTTCAACGCAGGACGCAAATGATTTAGCAg > 2:382902/1‑141 (MQ=255) ttttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaa < 2:313086/141‑1 (MQ=255) ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg < 2:163416/141‑1 (MQ=255) aaaaGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaa > 2:321759/1‑141 (MQ=255) gATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAaag < 2:30901/141‑1 (MQ=255) aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag > 2:427034/1‑141 (MQ=255) gCAACAAATAAAGGAAATGAAGAAGTTAAATGCTTAACAGAAGAACAAGGTAAAGAAAAATTAGAAGATACACATTCTGACGGCTCCACGCAGGATGCAAATGATTTAGCAGAAACTGCTGAAGCGCAAAAGAAGCCACgg > 2:278979/1‑140 (MQ=255) aaaTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGtt < 2:384052/141‑1 (MQ=255) aaTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTg > 2:394908/1‑141 (MQ=255) tAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACGAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaa < 1:4285/122‑1 (MQ=255) tAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACGAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaa > 2:4285/1‑122 (MQ=255) agaagTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGAttt > 1:55953/1‑141 (MQ=255) agTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTtga < 1:90504/141‑1 (MQ=255) tAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTtgatga > 1:230534/1‑141 (MQ=255) ccTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg > 1:373106/1‑141 (MQ=255) aaCAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCtt < 1:156088/141‑1 (MQ=255) gaagaaCAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAata < 1:156480/141‑1 (MQ=255) aaCAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCaa > 1:369141/1‑141 (MQ=255) aCAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAAt > 1:57978/1‑141 (MQ=255) aCAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTg > 2:37410/1‑141 (MQ=255) aaTTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTgaag > 2:188404/1‑141 (MQ=255) tAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGaaa > 1:263567/1‑141 (MQ=255) agaTACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGa > 1:427596/1‑141 (MQ=255) ttCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCaaaa < 1:31111/141‑1 (MQ=255) gAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATg > 2:268782/1‑141 (MQ=255) aGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATggg > 2:308848/1‑141 (MQ=255) gTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTg > 1:424451/1‑141 (MQ=255) tCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCt < 1:329534/141‑1 (MQ=255) cGCAGCACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCGCAAAAAATGGGTGCTAAAt < 1:278979/141‑1 (MQ=255) gACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTaaa > 2:61433/1‑141 (MQ=255) | GGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTAAA > CP000730/1250222‑1250497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |