BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,997	T→C	25.2%	T185T (ACA→ACG)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,997	0	T	C	25.2%	454.0 / 188.0	286	T185T (ACA→ACG)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (93/121); new base C (34/38); total (127/159)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 287 total aligned reads displayed.

CAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTT  >  CP000730/35867‑36137

cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                                                                                    >  1:428006/1‑141 (MQ=11)

  gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                                                  >  1:157082/1‑141 (MQ=11)

  gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                                                  >  2:210314/1‑141 (MQ=17)

  gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATCGATACtt                                                                                                                                  >  2:179274/1‑141 (MQ=17)

   tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                                                                                 >  2:307769/1‑141 (MQ=32)

    gtTCGC‑TAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                               <  2:157303/141‑1 (MQ=12)

     ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                                                                               >  1:362926/1‑141 (MQ=11)

     ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                               >  1:277888/1‑141 (MQ=17)

        gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                            <  2:142546/139‑1 (MQ=17)

          ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                          <  2:389943/141‑1 (MQ=17)

           tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCtt                                                                                                                         >  1:369480/1‑141 (MQ=11)

            aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                                                                                        <  1:191682/141‑1 (MQ=255)

              cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                      <  2:249247/141‑1 (MQ=32)

                tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                    <  1:173194/141‑1 (MQ=17)

                 gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                   <  2:404609/141‑1 (MQ=32)

                 gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACATGCGGTTC‑TCTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                   >  2:343059/1‑141 (MQ=17)

                  cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                  >  1:254881/1‑141 (MQ=17)

                     gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCTGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                                               <  1:435523/141‑1 (MQ=17)

                         gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                           >  2:83242/1‑141 (MQ=25)

                            gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                                                        >  1:70935/1‑141 (MQ=25)

                             cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                                                       >  1:241221/1‑141 (MQ=34)

                             cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                                                       <  1:328834/141‑1 (MQ=25)

                               aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTActatt                                                                                                     >  1:136981/1‑140 (MQ=21)

                               aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                     <  1:112727/141‑1 (MQ=25)

                                aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAGAGTATTCTTTGCTCTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATgc                                                                                                    >  2:412099/1‑140 (MQ=25)

                                aTTTCGTAGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                                                                                                    >  1:401850/1‑141 (MQ=25)

                                  ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                                                  <  1:276499/141‑1 (MQ=37)

                                    cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                <  1:263743/141‑1 (MQ=14)

                                    cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                <  2:197527/141‑1 (MQ=38)

                                        gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                            >  2:144197/1‑141 (MQ=14)

                                        gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                            >  1:124935/1‑141 (MQ=255)

                                        gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                            <  1:214452/141‑1 (MQ=37)

                                            ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                                                        <  2:44851/141‑1 (MQ=255)

                                              cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                       >  2:218087/1‑141 (MQ=255)

                                                 aaaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                   <  1:264873/141‑1 (MQ=255)

                                                 aaaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                   >  1:338546/1‑141 (MQ=37)

                                                  aaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                                                  <  1:354087/141‑1 (MQ=255)

                                                   aTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                 <  1:50440/141‑1 (MQ=39)

                                                   aTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                 <  2:182921/141‑1 (MQ=255)

                                                   aTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAACTGGTCTTTCTTACTTTAATATGACGGTGGCCTTGCTCAATGa                                                                                 >  1:347481/1‑141 (MQ=11)

                                                    tCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                                >  2:291737/1‑141 (MQ=255)

                                                    tCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                                <  1:301632/141‑1 (MQ=255)

                                                     ccGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                               >  2:436191/1‑141 (MQ=255)

                                                        tAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                            >  1:100003/1‑141 (MQ=255)

                                                             cTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                                                       <  1:289920/141‑1 (MQ=255)

                                                              tcttcagatctacGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                      >  1:22600/13‑141 (MQ=14)

                                                              tGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                      <  2:318029/141‑1 (MQ=39)

                                                              tGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACGTTAATATGACGGTGTTCTTGCTCAATGAGGTTATTCAGa                                                                      <  2:40343/141‑1 (MQ=255)

                                                              tGAAGAGACCTGAGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                      <  2:18630/141‑1 (MQ=255)

                                                                aaGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                                                    <  2:378833/141‑1 (MQ=255)

                                                                       cTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTGAGATATTTCGAt                                                             >  2:367133/1‑141 (MQ=255)

                                                                       cTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTCTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                                             <  2:371878/141‑1 (MQ=255)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           >  2:253412/1‑141 (MQ=255)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           >  1:185014/1‑141 (MQ=255)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           <  2:26718/141‑1 (MQ=255)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           <  2:35364/141‑1 (MQ=255)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           <  1:376278/141‑1 (MQ=38)

                                                                         acGGTTC‑TTTTTATATAGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           <  2:415101/140‑1 (MQ=255)

                                                                         acGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           >  2:53076/2‑141 (MQ=255)

                                                                         acGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           >  1:197855/2‑141 (MQ=255)

                                                                              tC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                      <  2:159507/141‑1 (MQ=255)

                                                                                 tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                    <  2:73885/141‑1 (MQ=40)

                                                                                     tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                <  2:2395/141‑1 (MQ=255)

                                                                                      atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTc                                               >  1:452318/1‑141 (MQ=255)

                                                                                         taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                            <  1:31209/141‑1 (MQ=255)

                                                                                          agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgt                                           >  2:118389/1‑141 (MQ=255)

                                                                                          agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgt                                           >  1:110405/1‑141 (MQ=255)

                                                                                            ggCGTAAATACACTCAATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATCTGACGGTGATATTGCTCAATGAGGTTATTCAGATATATCGATGTACAATGGCAGTCAGgttt                                         <  2:111165/140‑1 (MQ=14)

                                                                                             gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttta                                        <  2:48997/141‑1 (MQ=255)

                                                                                               gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                      <  2:443116/141‑1 (MQ=255)

                                                                                                  aaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                   <  1:206164/141‑1 (MQ=255)

                                                                                                  aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAGTGGCAGgtttaagttt                                   <  1:218979/141‑1 (MQ=14)

                                                                                                  aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                   <  1:105446/141‑1 (MQ=255)

                                                                                                   aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                  <  1:95550/141‑1 (MQ=255)

                                                                                                     aCATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAAAGTCAGGTTTAAGTTTaaa                                >  1:229868/1‑141 (MQ=255)

                                                                                                        ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                             <  2:148834/140‑1 (MQ=255)

                                                                                                        ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                             >  2:452061/2‑141 (MQ=255)

                                                                                                         tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                            <  2:138534/141‑1 (MQ=255)

                                                                                                         tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                            <  2:59807/141‑1 (MQ=255)

                                                                                                            ttaacTTTGAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                         <  2:128245/137‑1 (MQ=255)

                                                                                                            aTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATTACAGTCAGGTTTAAGTTTAAAAGCTTTa                         <  2:176085/141‑1 (MQ=255)

                                                                                                            aTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                         <  2:321762/141‑1 (MQ=255)

                                                                                                            aTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTa                         <  2:100003/141‑1 (MQ=255)

                                                                                                              aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                       <  2:426509/141‑1 (MQ=255)

                                                                                                                cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                     >  1:305756/1‑141 (MQ=255)

                                                                                                                cTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                     >  2:295838/1‑141 (MQ=255)

                                                                                                                     aaaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTa                <  1:236581/141‑1 (MQ=255)

                                                                                                                     aaaGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGCACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTa                >  2:134608/1‑141 (MQ=255)

                                                                                                                       aGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaac              >  1:415333/1‑139 (MQ=255)

                                                                                                                        gTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAcc             <  2:232537/141‑1 (MQ=255)

                                                                                                                          aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTATAAGTTTAAAAGCTTTAATTACTTTAACCAt           >  2:416408/1‑141 (MQ=255)

                                                                                                                             cTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGc        <  2:192929/141‑1 (MQ=40)

                                                                                                                              tttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt       <  1:86439/141‑1 (MQ=255)

                                                                                                                               ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa      >  1:129516/1‑141 (MQ=255)

                                                                                                                                 gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc    >  2:164836/1‑141 (MQ=255)

                                                                                                                                 gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc    <  2:430102/141‑1 (MQ=255)

                                                                                                                                 gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTAcc    <  1:382712/141‑1 (MQ=255)

                                                                                                                                  cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt   <  1:164240/141‑1 (MQ=255)

                                                                                                                                  cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt   <  1:39580/141‑1 (MQ=255)

                                                                                                                                  cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCATGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt   <  2:278181/141‑1 (MQ=255)

CAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTT  >  CP000730/35867‑36137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`