Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	CP000730	62,818	+CAATTT	intergenic (+83/+228)	USA300HOU_0056 → / ← speG	hypothetical protein/diamine N‑acetyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 62817	0 (0.000)	19 (0.550) +TCAATT	13/266	0.9	100%	intergenic (+82/+229)	USA300HOU_0056/speG	hypothetical protein/diamine N‑acetyltransferase
	?	CP000730	62818 =	0 (0.000)					intergenic (+83/+228)	USA300HOU_0056/speG	hypothetical protein/diamine N‑acetyltransferase

AAAAATAGAATTATTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATT  >  CP000730/62678‑62950                                                                                                                                             |                                                                                                                                     agaattattaaaacaacgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattt                                                                                                                                      <  2:100160‑M2/1‑1 (MQ=255)   aattattaaaacaacgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaatttat                                                                                                                                    >  1:266803‑M2/139‑141 (MQ=255)      tattaaaacaacgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTAtttt                                                                                                                                 <  1:129117‑M2/6‑1 (MQ=255)      tattaaaacaacgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatccctnaccgcaaagtgaaggatgctctcaattTAtttt                                                                                                                                 <  1:62895‑M2/6‑1 (MQ=255)         taaaacaacgcaaacaaggcgtactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTttgt                                                                                                                              <  2:143433‑M2/9‑1 (MQ=255)                    aaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAata                                                                                                                   >  2:5366‑M2/122‑141 (MQ=255)                    aaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAata                                                                                                                   >  2:292439‑M2/122‑141 (MQ=255)                          ggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCt                                                                                                             <  2:332165‑M2/26‑1 (MQ=255)                          ggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCt                                                                                                             <  1:181780‑M2/26‑1 (MQ=255)                                      aaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTgatag                                                                                                 <  2:403527‑M2/38‑1 (MQ=255)                                                   aattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATtaata                                                                                    <  2:266044‑M2/51‑1 (MQ=255)                                                           taacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTg                                                                            <  2:335269‑M2/59‑1 (MQ=255)                                                            aacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGc                                                                           <  1:198996‑M2/60‑1 (MQ=255)                                                                                gaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCgt                                                       <  1:219636‑M2/80‑1 (MQ=255)                                                                                  aaatagacaaaaataaattgtgagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCgttg                                                     <  1:105082‑M2/82‑1 (MQ=255)                                                                                  aaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCgttg                                                     <  1:158156‑M2/82‑1 (MQ=255)                                                                                                                                     tctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTAtt  <  1:5366‑M2/133‑1 (MQ=255)                                                                                                                                     tctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTAtt  <  1:292439‑M2/133‑1 (MQ=255)                                                                                                                                             |                                                                                                                                     AAAAATAGAATTATTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATT  >  CP000730/62678‑62950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.