Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,774,221 G→T A27D (GCC→GAC)  hemA ← glutamyl‑tRNA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,774,2210GT100.0% 50.0 / NA 18A27D (GCC→GAC) hemAglutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/13);  total (5/13)

TTGAATATAGTAACGACCTGTGTGAATTTGATCAACAACAGCATATACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGGCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAACATAAT  >  CP000730/1774091‑1774347
                                                                                                                                  |                                                                                                                              
acgAATATAGTACCGACCTGTTTGAATTTGATCAACAACAGCATCTACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAATATAGATTTAGTTTCAAATAAATCTTCCTAGGTAAGTCGTAAGTCATCctctct                                                                                                                      <  2:191369/139‑1 (MQ=255)
  gAATATAGTAACGACCTGTGTGAATTTGATCAACAACAGCATATACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTaa                                                                                                                    >  1:111998/1‑141 (MQ=255)
                                        gCATATACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCga                                                                              >  2:363341/1‑141 (MQ=255)
                                                                 ttGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAAt                                                     <  2:372433/141‑1 (MQ=255)
                                                                         atGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGa                                             <  2:111998/141‑1 (MQ=255)
                                                                         atGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGa                                             <  1:349803/141‑1 (MQ=255)
                                                                            aCATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAAc                                          <  1:137061/141‑1 (MQ=255)
                                                                              aTTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGc                                        <  1:302038/141‑1 (MQ=255)
                                                                              aTTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGc                                        <  1:225434/141‑1 (MQ=255)
                                                                                                aGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACaa                      <  1:272920/141‑1 (MQ=255)
                                                                                                           aaTCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAAtatta           >  1:9105/1‑141 (MQ=255)
                                                                                                             tCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATtata         <  2:47698/141‑1 (MQ=255)
                                                                                                               ttCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAAc       <  2:334578/141‑1 (MQ=255)
                                                                                                               ttCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAAc       <  2:87862/141‑1 (MQ=255)
                                                                                                               ttCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAAc       <  2:4359/141‑1 (MQ=255)
                                                                                                                tCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAACa      <  1:11800/141‑1 (MQ=255)
                                                                                                                    gggCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAACATAAt  >  2:202806/1‑141 (MQ=255)
                                                                                                                    gggCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAAAGCCCCCATATATACTAACAATACAAATATTATAACATAAt  >  1:327405/1‑141 (MQ=255)
                                                                                                                                  |                                                                                                                              
TTGAATATAGTAACGACCTGTGTGAATTTGATCAACAACAGCATATACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGGCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAACATAAT  >  CP000730/1774091‑1774347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: