New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 1713218	57 (0.860)	4 (0.060)	4/248	8.2	6.7%	coding (209/753 nt)	ycsF	possible lactam utilization protein
	?	CP000730	= 1713265	58 (0.930)					coding (162/753 nt)	ycsF	possible lactam utilization protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGATACAATGCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACTTGTAATTAACGGAAT  >  CP000730/1713079‑1713354                                                                                                                                            |                                                                                                                                         cTGATACAATGCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGcc                                                                                                                                         <  1:219160/141‑1 (MQ=255)   gATACAATGCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCtt                                                                                                                                       <  1:185997/141‑1 (MQ=255)   gATACAATGCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCtt                                                                                                                                       <  2:106314/141‑1 (MQ=255)    aTACAATGCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCTCCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCttt                                                                                                                                      >  2:142149/1‑141 (MQ=255)      aCAATGCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTAAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCa                                                                                                                                    >  2:456215/1‑141 (MQ=255)         aTGCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCACATTCTAACTGTGGCTGCCGGATAGTACTGCATGTCGTCTGCCAAAGCCTTTCAAAt                                                                                                                                 >  1:745725/1‑141 (MQ=255)           gCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCa                                                                                                                               <  1:359733/141‑1 (MQ=255)              ccATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTCGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGt                                                                                                                            <  2:558342/141‑1 (MQ=255)                     gTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAg                                                                                                                     <  1:231342/141‑1 (MQ=255)                         aaCATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGAtg                                                                                                                 <  1:619596/141‑1 (MQ=255)                          aCATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCANAGCCTTTCAAATNAGGTAAACCAGGAtgt                                                                                                                <  1:525034/141‑1 (MQ=255)                          aCATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGAtgt                                                                                                                >  2:188262/1‑141 (MQ=255)                                ttAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCt                                                                                                          >  1:739836/1‑141 (MQ=255)                                  aaTTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTAc                                                                                                        >  2:115685/1‑141 (MQ=255)                                     tttAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGc                                                                                                     >  1:353847/1‑141 (MQ=255)                                     tttAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGc                                                                                                     <  2:533504/141‑1 (MQ=255)                                         aCTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACa                                                                                                 >  2:174563/1‑141 (MQ=255)                                                   tGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCtt                                                                                       <  2:130232/141‑1 (MQ=255)                                                   tGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCtt                                                                                       <  1:98499/141‑1 (MQ=255)                                                    gCGACAAAACCCTTGTANTGCACCTAATNGATANATCNTCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTCCACCTACTGCAACATTATGTGCttt                                                                                      >  1:507042/1‑141 (MQ=255)                                                      gACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTgg                                                                                    <  1:271600/141‑1 (MQ=255)                                                              ccTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGtttt                                                                            <  1:560793/141‑1 (MQ=255)                                                                   tAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGt                                                                       <  2:422838/141‑1 (MQ=255)                                                                        cACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTttcat                                                                  >  1:463256/1‑141 (MQ=255)                                                                         aCCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTttcatt                                                                 >  2:199586/1‑141 (MQ=255)                                                                         aCCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTttcatt                                                                 >  1:357327/1‑141 (MQ=255)                                                                             aaTTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTcattcatt                                                             <  1:59930/141‑1 (MQ=255)                                                                             aaTTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTcattcatt                                                             >  1:665812/1‑141 (MQ=255)                                                                                tGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACa                                                          <  1:733665/141‑1 (MQ=255)                                                                                 gATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACAt                                                         >  1:400722/1‑141 (MQ=255)                                                                                 gATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACAt                                                         <  1:351851/141‑1 (MQ=255)                                                                                  aTAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACAtt                                                        >  2:750361/1‑141 (MQ=255)                                                                                       tcatcaAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTtcat                                                   >  2:746983/1‑141 (MQ=255)                                                                                       tcatcaAATTATAAATCTCGTCGTAAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTtcat                                                   <  1:456215/141‑1 (MQ=255)                                                                                           caAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGAtgt                                                                                                                >  2:176288/1‑76 (MQ=255)                                                                                           caAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGAtgt                                                                                                                <  1:176288/76‑1 (MQ=255)                                                                                           caAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGAtgt                                                                                                                <  1:176188/76‑1 (MQ=255)                                                                                           caAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGAtgt                                                                                                                >  2:176188/1‑76 (MQ=255)                                                                                            aaaTTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCa                                              >  2:241026/1‑141 (MQ=255)                                                                                             aaTTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAg                                             <  1:115685/141‑1 (MQ=255)                                                                                              aTTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGc                                            >  1:707187/1‑141 (MQ=255)                                                                                              aTTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGc                                            >  2:376333/1‑141 (MQ=255)                                                                                                tataAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCgc                                          <  1:750361/141‑2 (MQ=255)                                                                                                  taAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGa                                        >  1:293498/1‑141 (MQ=255)                                                                                                         cgtcgtTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACcac                                 <  1:142265/141‑1 (MQ=255)                                                                                                                  agaTATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACAt                        >  1:48466/1‑141 (MQ=255)                                                                                                                      atCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGc                    >  2:383224/1‑141 (MQ=255)                                                                                                                       tCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCa                   >  2:206104/1‑141 (MQ=255)                                                                                                                         tataTTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACt                 <  1:66386/141‑1 (MQ=255)                                                                                                                          atatTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACtt                <  2:269988/141‑1 (MQ=255)                                                                                                                          atatTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACtt                <  1:188262/141‑1 (MQ=255)                                                                                                                                 gTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACTTGTAATTa         >  1:725452/1‑141 (MQ=255)                                                                                                                                  tCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACTTGTAATTaa        >  1:178724/1‑141 (MQ=255)                                                                                                                                      ccAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACTTGTAATTAACGGa    <  2:639540/141‑1 (MQ=255)                                                                                                                                        aaaGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACTTGTAATTAACGGaat  <  1:89999/141‑1 (MQ=255)                                                                                                                                            ccTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACAtttt                                                      >  1:676051/1‑85 (MQ=255)                                                                                                                                            ccTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACAtttt                                                      <  2:676051/85‑1 (MQ=255)                                                                                                                                            |                                                                                                                                         CTGATACAATGCACCATGCGGTTTAACATGATTAATTTTAACTTGATGAATGCGACAAAACCCTTGTAATGCACCTAATTGATAAATCATCAAATTATAAATCTCGTCGTTAGAGATATCTATATTTCGTCTGCCAAAGCCTTTCAAATCAGGTAAACCAGGATGTGCACCTACTGCAACATTATGTGCTTTGGCAAGTTTTACCGTTTCATTCATTACATTTTCATCACCAGCGTGAAAACCACAAGCAACATTCGCACTTGTAATTAACGGAAT  >  CP000730/1713079‑1713354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.