Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,680,1851.T21.1% 45.0 / 12.7 19intergenic (+237/+295)cbpD/USA300HOU_2537possible secretory antigen/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (9/6);  new base T (2/2);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAATA  >  CP000730/2680055‑2680309
                                                                                                                                   |                                                                                                                              
ttGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAAttttt                                                                                                                      >  2:43527/1‑141 (MQ=255)
       tataTTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGtt                                                                                                             <  1:61565/141‑1 (MQ=255)
                             attcctatctatcATAAGCTATATATAATATTTTATGGTAATAGTGAAATAATACGGTATAATTTAACGTACGCTTTCGATGTAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCAATAGTAaaaaaa                                                                                       <  2:312704/129‑1 (MQ=255)
                                cATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAaata                                                                                    >  1:20193/1‑141 (MQ=255)
                                                  tatataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTga                                                                  <  1:192526/141‑1 (MQ=255)
                                                      taAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcag                                                                                   <  2:69854/122‑5 (MQ=255)
                                                       aaaaTTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcaga                                                                                  >  2:192923/1‑117 (MQ=255)
                                                       aaaaTTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcaga                                                                                  <  1:192923/122‑6 (MQ=255)
                                                                   tattatTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGCCCTGTTTAAATtgat                                                 >  1:282684/1‑141 (MQ=255)
                                                                            aataatTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTAAATTGATGACTCAAAAc                                       >  1:28713/1‑141 (MQ=255)
                                                                               aatTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCt                                     <  2:300768/141‑1 (MQ=255)
                                                                                        taAGTTAACATACGTTTTCGATATAAACCTTGTCCTAAATCTCAATAATTTTTTGCTGTTTTGTTCGTAATTAGGTGAGAAAAGAATCGATTGGCTGTTGTGAATCCTGTTTAGATGGATGTCTCAAAACGTTTATcgaac                            >  2:5101/1‑137 (MQ=255)
                                                                                        taACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTTCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCt                            >  2:116598/1‑141 (MQ=255)
                                                                                         aaCTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaat                                                                                      >  1:91095/1‑83 (MQ=255)
                                                                                                     gTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGttt               >  1:194662/1‑141 (MQ=255)
                                                                                                      ttttCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTa              >  1:284356/1‑141 (MQ=255)
                                                                                                        ttCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAAt            <  1:218491/141‑1 (MQ=255)
                                                                                                          cGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTg          >  1:273688/1‑141 (MQ=255)
                                                                                                                  aCCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAata  <  2:235264/141‑1 (MQ=255)
                                                                                                                                   |                                                                                                                              
TTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAATA  >  CP000730/2680055‑2680309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: