Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,319,519 G→A Q152* (CAA→TAA)  asp23 ← alkaline shock protein 23

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,319,5190GA100.0% 117.7 / NA 36Q152* (CAA→TAA) asp23alkaline shock protein 23
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/18);  total (18/18)

TTTCTATCAAATAATTAATAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTGTTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAATTCAGTTACTTTACGGAAGATTTTTGGTGCTGATTCACCAT  >  CP000730/2319382‑2319653
                                                                                                                                         |                                                                                                                                      
tttCTATCAAATAATTAATAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTAttt                                                                                                                                     >  1:324995/1‑141 (MQ=255)
tttCTATCAAATAATTAATAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTAttt                                                                                                                                     >  1:325049/1‑141 (MQ=255)
  tCTATCAAATAATTAATAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTcc                                                                                                                                   <  1:280378/141‑1 (MQ=255)
    tattaattatttgatAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCAc                                                                                                                                 >  1:190623/14‑141 (MQ=38)
    tATCAAATAATTAATAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCAc                                                                                                                                 >  1:320441/1‑141 (MQ=255)
    tATCAAATAATTAATAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCAc                                                                                                                                 <  1:38513/141‑1 (MQ=255)
        aaaTAATTAATAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAATCCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCtt                                                                                                                             <  2:269095/141‑1 (MQ=255)
               taatAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGt                                                                                                                      <  2:89822/141‑1 (MQ=255)
                  tAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTcc                                                                                                                                   <  2:289332/125‑1 (MQ=255)
                  tAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTAATTTcc                                                                                                                                   >  1:289332/1‑125 (MQ=255)
                         cAGCTTTTTTACATTTCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCg                                                                                                            >  2:21636/1‑141 (MQ=255)
                              tttttACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCAt                                                                                                                   >  1:10346/1‑129 (MQ=255)
                              tttttACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCAt                                                                                                                   <  2:10346/129‑1 (MQ=255)
                                 ttACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTg                                                                                                    <  1:23198/141‑1 (MQ=255)
                                      ttGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGt                                                                                               >  1:190285/1‑141 (MQ=255)
                                         ccTAAGAATTTAATGTCCCAAGCCCTTTTTATCGTATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTaa                                                                                            >  2:200280/1‑141 (MQ=255)
                                                  ttAATGTCCCAAGCCCTTCTTATCGAATAACTTATTGTAAACCTTGTCTTTATTGGTTATTGTTTTCGTTATTTTTTTCTTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCTTCAACTTGCATGTTAACTTCAACTa                                                                                   >  1:267265/1‑141 (MQ=255)
                                                   tAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTTAGTCATTTCATCGTCAACTTGCATGTTAACTTCAACTAc                                                                                  >  2:313322/1‑141 (MQ=255)
                                                    aaTGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACa                                                                                 >  2:205766/1‑141 (MQ=255)
                                                    aaTGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACa                                                                                 <  2:320441/141‑1 (MQ=255)
                                                           cAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAAc                                                                          <  1:200798/141‑1 (MQ=255)
                                                             aGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCa                                                                        <  1:279717/141‑1 (MQ=255)
                                                                 cTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACATGCATGTTAACTTCAACTACATCTAAACCAGTaa                                                                    >  1:186211/1‑141 (MQ=255)
                                                                       aTCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATAtt                                                              <  2:325049/141‑1 (MQ=255)
                                                                       aTCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATAtt                                                              <  2:324995/141‑1 (MQ=255)
                                                                        tCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTa                                                                             >  1:213681/1‑125 (MQ=255)
                                                                        tCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTa                                                                             <  2:213681/125‑1 (MQ=255)
                                                                                           ccTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAAt                                          >  1:87613/1‑141 (MQ=255)
                                                                                                   ttcttGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAATTCAGTTAc                                  <  1:21636/141‑1 (MQ=255)
                                                                                                     cttGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCaa                                                                                                        >  1:81745/1‑69 (MQ=255)
                                                                                                     cttGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCaa                                                                                                        <  2:81745/69‑1 (MQ=255)
                                                                                                            aTTGTTTTCGTTATTTTTTTCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAATTCAGTTACTTTACGGaa                         >  1:334575/1‑141 (MQ=255)
                                                                                                                         tttttttCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACATGCATGTTAACTTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAATTCAGTTACTTTACGGAAGATTTTTGGTGCt            <  2:186211/141‑1 (MQ=255)
                                                                                                                               tCGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAATTCAGTTACTTTACGGAAGATTTTTGGTGCTGATTCa      >  1:196269/1‑141 (MQ=255)
                                                                                                                                cGTGTTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTCCCCTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAATTCAGTTACTTTACGGAAGATTTTTGGTGCTGATTCAc     <  1:283529/141‑1 (MQ=255)
                                                                                                                                   gtTTTTATTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAATTCAGTTACTTTACGGAAGATTTTTGGTGCTGATTCACCat  <  2:156070/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TTTCTATCAAATAATTAATAGAAATCAGCTTTTTTACATTGCCTAAGAATTTAATGTCCCAAGCCCTTTTTATCGAATAACTTATTGTAAACCTTGTCTTTCTTGGTTATTGTTTTCGTTATTTTTTTCGTGTTTTTGTTTCCACTCTTTTTGAGTCATTACATCGTCAACTTGCATGTTAACTTCAACTACATCTAAACCAGTAATATATTTAACTTGTTCTTTAACTAATTCAGTTACTTTACGGAAGATTTTTGGTGCTGATTCACCAT  >  CP000730/2319382‑2319653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑