Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,373,558	A→T	M73K (ATG→AAG)	pbuG ←	NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,373,558	0	A	T	100.0%	93.4 / NA	29	M73K (ATG→AAG)	pbuG	NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/14);  total (15/14)

ATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCC  >  CP000730/2373424‑2373692                                                                                                                                       |                                                                                                                                       atatACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCTCTGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATa                                                                                                                                  <  2:297160/141‑1 (MQ=255)  tataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAg                                                                                                                                 <  1:20469/141‑1 (MQ=255)   ataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGc                                                                                                                                >  1:267684/1‑141 (MQ=255)               aaaaCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAAt                                                                                                                    <  2:226640/141‑1 (MQ=255)                aaaCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAAtg                                                                                                                   <  2:29044/141‑1 (MQ=255)                     ccTGTTACACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTCTTAGCCCCTTGACTAGCGAGCATACAAATgctgct                                                                                                              >  1:55669/1‑141 (MQ=255)                      cTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTa                                                                                                             <  1:311949/141‑1 (MQ=255)                          tAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGc                                                                                                         >  1:190911/1‑141 (MQ=255)                               cAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATgaaaaaa                                                                                                    <  2:217941/141‑7 (MQ=255)                                 aCTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCt                                                                                                  >  2:25279/1‑141 (MQ=255)                                     gCCAAGGAATACCCATGGTTAACACAACAGTAAATGCACAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTAcca                                                                                              <  2:72230/141‑3 (MQ=255)                                     gCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACaa                                                                                              <  1:185891/141‑1 (MQ=255)                                         aGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATAGTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAAt                                                                                          <  1:217619/141‑1 (MQ=255)                                                cccATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCt                                                                                   >  1:35313/1‑141 (MQ=255)                                                      gTTAACACGACAGTAAATGCAAAGAATGCATTTAATCCCATGCCTGTTGCTAACGCAATTGGATATTTAGCTATTATCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCATTCGCTACAAAAATggaccaaggtgcc                                                                             <  2:190426/141‑12 (MQ=255)                                                               acaGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCata                                                                    >  2:328353/1‑141 (MQ=255)                                                                   tAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtctt                                                                <  2:267684/141‑1 (MQ=255)                                                                     aaTGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtcttct                                                              >  2:291450/1‑141 (MQ=255)                                                                       tGCAAAGAATGCAGTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTCGTCCATTCTCATGTCTTCTGa                                                            >  2:88846/1‑141 (MQ=255)                                                                        gCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACCAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGAt                                                           <  2:72595/141‑1 (MQ=255)                                                                         cattctttGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATa                                                          >  2:300784/8‑141 (MQ=255)                                                                          aaaGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGACAAAACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATAc                                                         <  2:259243/141‑1 (MQ=255)                                                                                   aTTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAac                                                >  2:138688/1‑141 (MQ=255)                                                                                         tCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGc                                          >  2:92579/1‑141 (MQ=255)                                                                                            cATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTaa                                       >  2:87232/1‑141 (MQ=255)                                                                                                ccTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACtt                                   <  1:270060/141‑1 (MQ=255)                                                                                                    gTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTaaaa                               >  1:329424/1‑141 (MQ=255)                                                                                                                       tttAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTaa            >  2:281173/1‑141 (MQ=255)                                                                                                                                 aGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAatagtgtat  >  1:319840/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                       ATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCC  >  CP000730/2373424‑2373692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑