| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 2,026,955 | G→A | T331I (ACA→ATA) | vraS ← | sensor histidine kinase VraS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,026,955 | 0 | G | A | 96.3% | 81.7 / NA | 27 | T331I (ACA→ATA) | vraS | sensor histidine kinase VraS |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (18/8); minor base C (1/0); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCT > CP000730/2026822‑2027088 | actactTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGa < 1:20455/141‑1 (MQ=255) tCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTgg < 2:170541/141‑1 (MQ=255) tCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTgg > 1:205686/1‑141 (MQ=255) gTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATg > 1:158931/1‑141 (MQ=255) gTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATg > 1:99014/1‑141 (MQ=255) ttGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATgg < 1:273736/141‑1 (MQ=255) aaCTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCa < 2:198803/141‑1 (MQ=255) ttGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCa > 1:157810/1‑141 (MQ=255) aaaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGAAAC‑ATCTGGTAACGTTGCACCAAtt > 1:177937/1‑141 (MQ=255) aaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAAttt > 1:153670/1‑141 (MQ=255) cGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCt < 2:94999/91‑1 (MQ=255) cGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCt > 1:94999/1‑91 (MQ=255) ccATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTTATGTTGTTCTGGCCCGGATTCGGATGCCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCa > 1:155659/1‑141 (MQ=255) ccATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCa > 1:321891/1‑141 (MQ=255) aTTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACg < 1:66523/141‑1 (MQ=255) cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACATCGATACGTATACCTGAATCTGGCATTGATACAATATGG‑ATCGTTGCACCAATTTCTAAAGCTCTTTCACGCcta > 1:286785/1‑141 (MQ=255) aTGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCatat > 1:278020/1‑141 (MQ=255) aCTTTAATAGACATACGAATCCTCCTTATTTAAAGGTGATTTCACATCGCTCCGTCTACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCAATCACttta > 2:293379/1‑140 (MQ=255) aTCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTaa > 1:254755/1‑141 (MQ=255) cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt > 1:10628/1‑141 (MQ=255) cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt > 1:246420/1‑141 (MQ=255) aCGAATCCTCCTTATTTAAAGGTGCTTTCACGTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATCTTTTTAAGTCCATAACTTTGTTCTAATTTTtcaa > 2:174479/1‑140 (MQ=255) agtcctccTTGTTTAAATGTGCGTTCTCCTCGATACGTATACCTGAATCGCGAAATGATACAATATGG‑AACCTTGCACCAATTTCCCAAGCCCTTTCACGCATATTTTTAAGTCCATAACTATTTTCTAATTTTtcatcca < 2:137103/139‑3 (MQ=255) cctTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTaa < 1:342059/141‑1 (MQ=255) aaaGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTa > 1:58008/1‑141 (MQ=255) gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTa > 1:14856/1‑141 (MQ=255) gATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATtct > 2:266706/1‑141 (MQ=255) | ACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCT > CP000730/2026822‑2027088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |