Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,857,170 G→A G195R (GGA→AGA)  nixA → NiCoT family nickel (Ni2+)‑cobalt (Co2+) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,857,1700GA95.8% 64.2 / ‑3.4 25G195R (GGA→AGA) nixANiCoT family nickel (Ni2+)‑cobalt (Co2+) transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (8/15);  total (8/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

CGTCGTGAACACATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGATTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTGGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGAGTTTA  >  CP000730/2857038‑2857295
                                                                                                                                    |                                                                                                                             
cgtcgtGAACACATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGATTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGt                                                                                                                       >  1:130337/1‑141 (MQ=255)
        acacATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGATTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATAc                                                                                                               <  1:65452/141‑1 (MQ=255)
                           gTCGATGCATTACTTGAATCTAGAGGATTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTa                                                                                            >  1:760/1‑141 (MQ=255)
                                tGCATTACTTGAATCTAGAGGATTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGc                                                                                       >  2:87354/1‑141 (MQ=255)
                                                     aTTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATcaca                                                                  <  1:35931/141‑1 (MQ=255)
                                                     aTTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATcaca                                                                  <  1:202876/141‑1 (MQ=255)
                                                       tGGTTTCTGGATGTGTAGGACTTTATTGCAAATTAATCTCGCGTAGTTGGCACGTATTGCAACTTGGCTTTTTATTTTGACTTGGTTTTGGTACAGCTAGTGAAATTGCGTTATTCGCTTTTTCTTCAGGTGCATCacaac                                                                <  2:82910/141‑1 (MQ=255)
                                                       tGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCacaac                                                                <  2:60949/141‑1 (MQ=255)
                                                          tttCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAg                                                             >  1:156921/1‑141 (MQ=255)
                                                               cGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGTCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCGGGCGCATCACAACAAGCcatt                                                        >  2:221093/1‑141 (MQ=255)
                                                                gATTTGTAGGACCTTATTTCAAGTTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTGGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCcattt                                                       <  1:117722/141‑1 (MQ=255)
                                                                   ttGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCcatttcat                                                    >  2:97602/1‑141 (MQ=255)
                                                                    tGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCcatttcatt                                                   <  1:125943/141‑1 (MQ=255)
                                                                     gTAGGACCGTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCcatttcattt                                                  >  2:43375/1‑141 (MQ=255)
                                                                                               gcgTAGTTGGCACTTATTGCCACTTGGGTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCTCAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAAtgtt                        <  2:147401/141‑1 (MQ=255)
                                                                                               gcgTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGCCTTGGTTTTGATCCAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAAtttt                        <  2:122036/141‑1 (MQ=255)
                                                                                                 gTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTAt                      <  1:2373/141‑1 (MQ=255)
                                                                                                    gTTGGCACGTATTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCg                   >  2:11017/1‑141 (MQ=255)
                                                                                                              aTTGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCAGCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTAt         <  2:148848/141‑1 (MQ=255)
                                                                                                               ttGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATg        <  1:19186/141‑1 (MQ=255)
                                                                                                               ttGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATg        <  2:760/141‑1 (MQ=255)
                                                                                                                tGCCACTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGa       <  1:69688/141‑1 (MQ=255)
                                                                                                                    aCTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGAGttt   <  1:11017/141‑1 (MQ=255)
                                                                                                                     cTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGAGTTTa  <  2:173164/141‑1 (MQ=255)
                                                                                                                     cTTGGCTTTTTATTTAGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGAGTTTa  <  2:93541/141‑1 (MQ=255)
                                                                                                                                    |                                                                                                                             
CGTCGTGAACACATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGATTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTGGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGAGTTTA  >  CP000730/2857038‑2857295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: