Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,201,454 T→C 18.2% V144A (GTT→GCT)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,201,4540TC18.2% 42.0 / 6.2 22V144A (GTT→GCT) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (10/8);  new base C (2/2);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

GAACAAGCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGTCTCCTTCAAGTGAGT  >  CP000730/1201327‑1201594
                                                                                                                               |                                                                                                                                            
gAACAAGCATGAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCCACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACttt                                                                                                                                 <  1:91429/141‑1 (MQ=255)
     aGCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGa                                                                                                                            >  1:72685/1‑141 (MQ=255)
      gCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGAt                                                                                                                           >  1:112507/1‑141 (MQ=255)
           aaCGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGCTCGTGGTGACTTTAATGATCCata                                                                                                                      >  2:90539/1‑141 (MQ=255)
           aaCGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGCTCGTGGTGACTTTAATGATCCata                                                                                                                      >  2:19351/1‑141 (MQ=255)
            aCGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCatat                                                                                                                     <  1:134699/141‑1 (MQ=255)
                 aaaaGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTAc                                                                                                                >  2:191689/1‑141 (MQ=255)
                                  agaaaATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACg                                                                                               >  1:129745/1‑141 (MQ=255)
                                      aaTGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGc                                                                                           >  1:219930/1‑141 (MQ=255)
                                       aTGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCt                                                                                          <  1:94136/141‑1 (MQ=255)
                                       aTGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCt                                                                                          >  1:47444/1‑141 (MQ=255)
                                            aaCAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGcaca                                                                                     >  2:4966/1‑141 (MQ=255)
                                                     aTTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTAt                                                                            >  2:31320/1‑141 (MQ=255)
                                                                 aTGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAAt                                                                <  2:135887/141‑1 (MQ=255)
                                                                 aTGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAAt                                                                <  2:148075/141‑1 (MQ=255)
                                                                   gTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGCTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATgg                                                              <  1:19351/141‑1 (MQ=255)
                                                                   gTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGCTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATgg                                                              <  1:90539/141‑1 (MQ=255)
                                                                        gAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGAt                                                         <  1:189719/141‑1 (MQ=255)
                                                                                                  aCAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTaaaaa                               >  1:19078/1‑141 (MQ=255)
                                                                                                                 tAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGtc                <  1:163546/141‑1 (MQ=255)
                                                                                                                 tAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGtc                <  2:72685/141‑1 (MQ=255)
                                                                                                                   gACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGtctc              >  2:89438/1‑141 (MQ=255)
                                                                                                                               ttCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGTCTCCTTCAAGTGAgg  >  2:25379/1‑140 (MQ=255)
                                                                                                                               ttCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGTCTCCTTCAAGTGAGt  >  2:21192/1‑141 (MQ=255)
                                                                                                                               |                                                                                                                                            
GAACAAGCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGTCTCCTTCAAGTGAGT  >  CP000730/1201327‑1201594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: