Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,358,670 T→C 10.0% K83R (AAA→AGA)  rpoA ← DNA‑directed RNA polymerase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,358,6700TC10.0% 164.1 / 10.7 60K83R (AAA→AGA) rpoADNA‑directed RNA polymerase alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (23/31);  new base C (3/3);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

TGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGATACGACGTAAGGA  >  CP000730/2358531‑2358803
                                                                                                                                           |                                                                                                                                     
tGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGttt                                                                                                                                      <  1:87117/141‑1 (MQ=255)
tGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGttt                                                                                                                                      <  1:294791/141‑1 (MQ=255)
          ttAAGCTNTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTCTAATGTTCata                                                                                                                            <  2:345686/141‑1 (MQ=255)
          ttAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCata                                                                                                                            <  2:46644/141‑1 (MQ=255)
          ttAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTCTAATGTTCata                                                                                                                            <  1:61848/141‑1 (MQ=255)
          ttAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTCTAATGTTCata                                                                                                                            <  1:188197/141‑1 (MQ=255)
             aGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCGATGCTAATTGTTTAATGTTCataata                                                                                                                         >  2:364097/1‑141 (MQ=255)
             aGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCataata                                                                                                                         >  2:51808/1‑141 (MQ=255)
             aGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCataata                                                                                                                         >  2:356647/1‑141 (MQ=255)
               ctctGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCTGAGTCTATTTTCACTGCTAATTGTTTAATGTTCATaataat                                                                                                                       >  1:319816/1‑141 (MQ=255)
                      tttAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGaa                                                                                                                >  2:155535/1‑141 (MQ=255)
                      tttAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGaa                                                                                                                >  2:454239/1‑141 (MQ=255)
                      tttAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGaa                                                                                                                >  2:271579/1‑141 (MQ=255)
                         aaaaTTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACa                                                                                                             <  1:257134/141‑1 (MQ=255)
                           aaTTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGCAGAAACATc                                                                                                           <  1:405598/141‑1 (MQ=255)
                                   cATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTa                                                                                                   <  2:190502/141‑1 (MQ=255)
                                   cATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTa                                                                                                   <  2:190517/141‑1 (MQ=255)
                                   cATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTa                                                                                                   <  2:297233/141‑1 (MQ=255)
                                         tATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGt                                                                                             <  2:332274/141‑1 (MQ=255)
                                         tATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGt                                                                                             <  2:385975/141‑1 (MQ=255)
                                                tgtAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTAAATGCTAATTGTTTAACGTTCATAATAATTGTAAAAACAACTTCACCTACATTGTCTActgc                                                                                      >  1:71731/1‑141 (MQ=255)
                                                    aTGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCTATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTgag                                                                                  >  1:243552/1‑141 (MQ=255)
                                                     tGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTgaga                                                                                 >  2:48091/1‑141 (MQ=255)
                                                     tGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTgaga                                                                                 >  2:112328/1‑141 (MQ=255)
                                                     tGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTgaga                                                                                 >  2:253485/1‑141 (MQ=255)
                                                       tCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAAt                                                                               <  2:84993/141‑1 (MQ=255)
                                                       tCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAAt                                                                               <  2:205922/141‑1 (MQ=255)
                                                       tCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAAt                                                                               <  2:276939/141‑1 (MQ=255)
                                                            tGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGTATTCATg                                                                          <  1:364097/141‑1 (MQ=255)
                                                            tGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATg                                                                          <  1:356647/141‑1 (MQ=255)
                                                            tGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATg                                                                          <  1:51808/141‑1 (MQ=255)
                                                                    cTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTc                                                                  >  2:453125/1‑141 (MQ=255)
                                                                    cTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTc                                                                  >  2:16222/1‑141 (MQ=255)
                                                                     ttCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTcc                                                                 <  1:102597/141‑1 (MQ=255)
                                                                         ccTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCa                                                             >  1:93253/1‑141 (MQ=255)
                                                                         ccTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCa                                                             >  1:125616/1‑141 (MQ=255)
                                                                            tcatcaCGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAAtt                                                          <  2:133871/141‑1 (MQ=255)
                                                                            tcatcaCGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAAtt                                                          >  2:93902/1‑141 (MQ=255)
                                                                            tcatcaCGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAAtt                                                          >  1:236973/1‑141 (MQ=255)
                                                                            tcatcaCGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAAtt                                                          >  1:344289/1‑141 (MQ=255)
                                                                            tcatcaCGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAAtt                                                          >  2:280557/1‑141 (MQ=255)
                                                                               tcaCGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCa                                                       >  1:63769/1‑141 (MQ=255)
                                                                                   gTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAatat                                                   <  2:437005/141‑1 (MQ=255)
                                                                                            tttCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACgg                                          <  2:319816/141‑1 (MQ=255)
                                                                                              tCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCt                                        <  1:260718/141‑1 (MQ=255)
                                                                                                  aaGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCAc                                    >  1:359105/1‑141 (MQ=255)
                                                                                                   aGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTCTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCAcc                                   >  1:287824/1‑141 (MQ=255)
                                                                                                   aGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTCTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCAcc                                   >  1:287835/1‑141 (MQ=255)
                                                                                                      tttATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTgg                                <  2:297497/141‑1 (MQ=255)
                                                                                                      tttATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTgg                                <  2:243552/141‑1 (MQ=255)
                                                                                                      tttATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACGCCCTCAATTTCAATATACTTAACGGCTGCACCTgg                                <  2:269076/141‑1 (MQ=255)
                                                                                                       ttATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGt                               <  2:63769/141‑1 (MQ=255)
                                                                                                           cttcttCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATg                           >  2:48544/1‑141 (MQ=255)
                                                                                                                  agagTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGAtag                    <  1:104602/141‑1 (MQ=255)
                                                                                                                  agagTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGAtag                    <  1:52874/141‑1 (MQ=255)
                                                                                                                       aaaTTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGa               <  2:313759/141‑1 (MQ=255)
                                                                                                                       aaaTTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGa               <  1:268503/141‑1 (MQ=255)
                                                                                                                                 tGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGATACGACGTaa     <  1:182233/141‑1 (MQ=255)
                                                                                                                                 tGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGATACGACGTaa     <  1:112328/141‑1 (MQ=255)
                                                                                                                                 tGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGATACGACGTaa     <  1:48091/141‑1 (MQ=255)
                                                                                                                                 tGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGATACGACGTaa     <  1:253485/141‑1 (MQ=255)
                                                                                                                                    tAATTGTCTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGATACGACGTAAGGa  >  1:257613/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                     
TGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTGAGAATTCATGTAAAACTCCCTCAATTTCAATATACTTAACGGCTGCACCTGGTAATGAAGATAGTAGGATACGACGTAAGGA  >  CP000730/2358531‑2358803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: