Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,656,802 A→T 6.4% S72R (AGT→AGA USA300HOU_2510 ← MarR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,656,8020AT 6.4% 191.6 / 5.7 64S72R (AGT→AGAUSA300HOU_2510MarR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (30/29);  new base T (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

AACGCTTTTGTTAATGTCTCAGCATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTACGTCTTCTTT  >  CP000730/2656662‑2656942
                                                                                                                                            |                                                                                                                                            
aaCGCTTTTGTTAATGTCTCAGCATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGa                                                                                                                                              <  2:423151/141‑1 (MQ=255)
   gCTTTTGTTAATGTCTCAGCATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACtt                                                                                                                                           >  1:388763/1‑141 (MQ=255)
   gCTTTTGTTAATGTCTCAGCATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACtt                                                                                                                                           >  1:253127/1‑141 (MQ=255)
    cTTTTGTTAATGTCTCAGCATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTa                                                                                                                                          <  2:95752/141‑1 (MQ=255)
                tctcATCATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGaaa                                                                                                                              <  1:177768/141‑1 (MQ=255)
                    aGCATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAAtgct                                                                                                                          <  2:314802/141‑1 (MQ=255)
                      cATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAAtgctgc                                                                                                                        <  2:13091/141‑1 (MQ=255)
                        tGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTa                                                                                                                      >  1:313687/1‑141 (MQ=255)
                          cTTAGGGNAAATATCTGCCATTTGGCTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACt                                                                                                                    <  2:345698/141‑1 (MQ=255)
                          cTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACt                                                                                                                    <  2:210399/141‑1 (MQ=255)
                          cTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACt                                                                                                                    <  1:232772/141‑1 (MQ=255)
                          cTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACt                                                                                                                    <  2:315499/141‑1 (MQ=255)
                          cTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACt                                                                                                                    <  2:390013/141‑1 (MQ=255)
                              gggAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCa                                                                                                                >  1:406271/1‑141 (MQ=255)
                              gggAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCa                                                                                                                >  1:301889/1‑141 (MQ=255)
                              gggAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACAAATAAAATACTGCTACTTGCa                                                                                                                >  1:370811/1‑141 (MQ=255)
                                   aaTATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTaa                                                                                                           <  2:98055/141‑1 (MQ=255)
                                      atCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATAc                                                                                                        <  1:356368/141‑1 (MQ=255)
                                      atCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATAc                                                                                                        <  1:14346/141‑1 (MQ=255)
                                      atCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATAc                                                                                                        >  2:386929/1‑141 (MQ=255)
                                           ccATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGTCTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGt                                                                                                   >  2:182306/1‑141 (MQ=255)
                                           ccATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGTCTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGt                                                                                                   >  2:427415/1‑141 (MQ=255)
                                              tttgactttgacCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGtctc                                                                                                <  1:52328/141‑1 (MQ=255)
                                                   ctttgacCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATa                                                                                           >  1:174099/1‑141 (MQ=255)
                                                   ctttgacCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATa                                                                                           >  1:260966/1‑141 (MQ=255)
                                                   ctgtgacCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAACAAGCTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATa                                                                                           >  1:145660/4‑141 (MQ=255)
                                                            ttttCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAAtt                                                                                  <  2:324681/141‑1 (MQ=255)
                                                            ttttCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAAtt                                                                                  <  2:330587/141‑1 (MQ=255)
                                                               tCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTACTTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGt                                                                               >  2:655/1‑141 (MQ=255)
                                                               tCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACCTATGAAATGCTGCTACTTGCAATTAATACGCGTTCTCTAATACGTTAAATTGGt                                                                               >  2:206630/1‑141 (MQ=255)
                                                                      aaCAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGAc                                                                        <  2:253127/141‑1 (MQ=255)
                                                                      aaCAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGAc                                                                        <  2:388763/141‑1 (MQ=255)
                                                                               tatataCACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTtattat                                                               >  2:228293/1‑141 (MQ=255)
                                                                               tatataCACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTtattat                                                               >  2:66039/1‑141 (MQ=255)
                                                                               tatataCACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTtattat                                                               >  1:170402/1‑141 (MQ=255)
                                                                               tatataCACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAAACTTTGTCCTCTAATTCACTTACAACATATTGATTACAGCGACGTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTtattat                                                               >  1:388965/1‑141 (MQ=255)
                                                                                atataCACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATtata                                                              <  1:7428/141‑1 (MQ=255)
                                                                                      acGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCaa                                                        >  2:252753/1‑141 (MQ=255)
                                                                                            ctcatcCTTATCCTTTCCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTTCAATTAATACGCGGTCTCTAATACGTTGAATTTGTTGCGGACCTTTATTATAAAGCAACTCgcg                                                  >  2:104110/2‑139 (MQ=255)
                                                                                            atcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTTATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCgcg                                                  >  2:377475/1‑139 (MQ=255)
                                                                                            atcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTTATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAg                                                  >  2:167204/1‑141 (MQ=255)
                                                                                            atcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAg                                                  >  2:221932/1‑141 (MQ=255)
                                                                                             tcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGc                                                 >  1:283650/1‑141 (MQ=255)
                                                                                             tcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGc                                                 >  2:111454/1‑141 (MQ=255)
                                                                                             tcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGc                                                 >  1:299824/1‑141 (MQ=255)
                                                                                             tcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGc                                                 >  2:338364/1‑141 (MQ=255)
                                                                                             tcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGc                                                 >  1:47879/1‑141 (MQ=255)
                                                                                             tcatcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACATTTATAATAAAGCAACTCGAGc                                                 >  1:370974/1‑141 (MQ=255)
                                                                                              catcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGTCTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCa                                                <  1:182306/141‑1 (MQ=255)
                                                                                              catcTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGTCTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCa                                                <  1:427415/141‑1 (MQ=255)
                                                                                                  tttATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGc                                            <  2:284046/141‑1 (MQ=255)
                                                                                                  tttATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGc                                            <  2:330444/141‑1 (MQ=255)
                                                                                                      tCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAAt                                        >  1:35088/1‑141 (MQ=255)
                                                                                                      tCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAAt                                        >  1:160674/1‑141 (MQ=255)
                                                                                                       ccTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAAtt                                       <  2:328517/141‑1 (MQ=255)
                                                                                                             cACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTaa                                 >  2:75705/1‑141 (MQ=255)
                                                                                                                          aCCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCata                    <  2:145660/141‑1 (MQ=255)
                                                                                                                           ccTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCatat                   <  2:220890/141‑1 (MQ=255)
                                                                                                                                gTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCtt              <  2:174099/141‑1 (MQ=255)
                                                                                                                                gTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCtt              <  2:260966/141‑1 (MQ=255)
                                                                                                                                  ccTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTa            >  2:403398/1‑141 (MQ=255)
                                                                                                                                    tctAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTACg          <  1:73118/141‑1 (MQ=255)
                                                                                                                                          tGACTTACAACGTATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTACGtcttct    <  2:457701/141‑1 (MQ=255)
                                                                                                                                          tGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCTACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTACGtcttct    <  1:234561/141‑1 (MQ=255)
                                                                                                                                          tGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGGGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTACGtcttct    <  2:30582/141‑1 (MQ=255)
                                                                                                                                            aCTTANAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCATCTCGAGCACTGCAAATTCAGNAATATTTAAGCCATATCGCTTTACGTCTTCttt  <  1:71583/141‑1 (MQ=255)
                                                                                                                                            aCTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTACGTCTTGttt  >  1:189338/1‑141 (MQ=255)
                                                                                                                                            aCTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTACGTCTTCttt  >  1:377537/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AACGCTTTTGTTAATGTCTCAGCATGCTTAGGGAAAATATCTGCCATTTGACTTTGACCTTTTTCAGTTAAACAAGCCATATATACACGTTTATCATCTTTATCCTTTTCACGTGTAATCCAACCTTTGTCCTCTAATTGACTTACAACATATGAAATGCTGCTACTTGCAATTAATACGCGGTCTCTAATACGTTGAATTGGTTGCGGACCTTTATTATAAAGCAACTCGAGCACTGCAAATTCAGTAATATTTAAGCCATATCGCTTTACGTCTTCTTT  >  CP000730/2656662‑2656942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: