Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 264,793 T→C 8.2% L336L (CTT→CTC glpQ1 → possible glycerophosphodiester phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730264,7930TC 8.2% 133.0 / 4.1 49L336L (CTT→CTCglpQ1possible glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (26/19);  new base C (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

ATTTATTTACGAAATTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGAAAGCTGCTG  >  CP000730/264654‑264929
                                                                                                                                            |                                                                                                                                        
atttatttACGAAATTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTg                                                                                                                                         <  2:124026/141‑1 (MQ=255)
 tttatttaCGAAATTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGc                                                                                                                                        >  2:134014/1‑141 (MQ=255)
   tatttaCGAAATTATCATTAATCAGCGTGTTAGTCCTGCACTTAAGACAAGCGACTGTAATAGCCCAACCTGGCTTAGATTTAAATGATCCAACACCGAAACGGAAGGCTAGGTTCTTTATCATTTCAATGGTGCTTGCAg                                                                                                                                      <  1:18854/141‑1 (MQ=255)
    atttaCGAAATTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGt                                                                                                                                     >  2:412138/1‑141 (MQ=255)
         cGAAATTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACAt                                                                                                                                >  1:397566/1‑141 (MQ=255)
         cGAAATTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACAt                                                                                                                                >  1:354454/1‑141 (MQ=255)
           aaaTTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGt                                                                                                                              >  1:284016/1‑141 (MQ=255)
           aaaTTATCATCAATCAGAGTGTTAGTACTGCACTTAAAACAAAAGAATGTACTCCACAAACCGGGCATAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGCTGCCTCCAGCGCCATGt                                                                                                                              >  2:437592/1‑141 (MQ=255)
            aaTTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGtt                                                                                                                             <  1:102188/141‑1 (MQ=255)
            aaTTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGtg                                                                                                                             <  1:437181/141‑2 (MQ=255)
                 tCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATc                                                                                                                        <  1:188867/141‑1 (MQ=255)
                   aTTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCgg                                                                                                                      >  2:22441/1‑141 (MQ=255)
                   aTTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCgg                                                                                                                      >  2:200560/1‑141 (MQ=255)
                         cAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACGGGAACTTAAGTCGAGTGTCTTTATAATTTCAATGGTGCTCGCAGTGACATGTTTTATCGGTTATaa                                                                                                                >  1:231044/1‑141 (MQ=255)
                         cAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTCGCAGTGACATGTTTTATCGGTTATaa                                                                                                                >  1:330664/1‑141 (MQ=255)
                               gtTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGta                                                                                                          <  1:50575/141‑1 (MQ=255)
                                ttAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGtac                                                                                                         <  2:406922/141‑1 (MQ=255)
                                            ttAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACaat                                                                                             >  2:122183/1‑141 (MQ=255)
                                                aaCAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAataata                                                                                         >  1:227467/1‑141 (MQ=255)
                                                           gTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACa                                                                              >  2:247031/1‑141 (MQ=255)
                                                                        cGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCatta                                                                 >  2:268453/1‑141 (MQ=255)
                                                                        cGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCatta                                                                 >  2:138245/1‑141 (MQ=255)
                                                                          ggCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCattatt                                                               >  2:330734/1‑141 (MQ=255)
                                                                           gCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTg                                                              >  1:212618/1‑141 (MQ=255)
                                                                                  aTTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCg                                                       <  1:363309/141‑1 (MQ=255)
                                                                                         tatCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGCGGTTTc                                                >  2:307026/1‑141 (MQ=255)
                                                                                                aaaCCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGAt                                          <  1:22441/141‑1 (MQ=255)
                                                                                                aaaCCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATAAAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGAt                                          <  1:200560/141‑1 (MQ=255)
                                                                                                    cGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAg                                      >  2:208776/1‑141 (MQ=255)
                                                                                                    cGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAg                                      >  2:272687/1‑141 (MQ=255)
                                                                                                       aatggAAGTCTAGGTTCTTTATAATTTCAATGGTGCTCGCAGTGACCTGTTTTATCGGTTCTCCCATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAACGGCg                                   <  2:231044/138‑1 (MQ=255)
                                                                                                       aaCGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTCGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCg                                   <  2:330664/141‑1 (MQ=255)
                                                                                                        aCGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGt                                  <  1:247973/141‑1 (MQ=255)
                                                                                                        aCGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGt                                  <  1:45181/141‑1 (MQ=255)
                                                                                                        aCGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGt                                  <  1:155541/141‑1 (MQ=255)
                                                                                                        aCGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGt                                  >  1:259277/1‑141 (MQ=255)
                                                                                                                   ggTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTa                       >  2:373904/1‑141 (MQ=255)
                                                                                                                        tttATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCg                  <  2:220084/141‑1 (MQ=255)
                                                                                                                         ttATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGt                 <  1:171981/141‑1 (MQ=255)
                                                                                                                         ttATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGt                 <  1:117616/141‑1 (MQ=255)
                                                                                                                         ttATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGt                 <  1:149914/141‑1 (MQ=255)
                                                                                                                         ttATAATTTCAATGGTGCTTGCAGTGACATGTTTTAGCGGCTATAACAGGTACGGGCTTTACAAGAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGt                 <  1:183473/141‑1 (MQ=255)
                                                                                                                               tttCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGa           <  1:134014/141‑1 (MQ=255)
                                                                                                                                  cAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGAAAg        >  2:22771/1‑141 (MQ=255)
                                                                                                                                    aTGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGAAAgct      >  2:367093/1‑141 (MQ=255)
                                                                                                                                    aTGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGAAAgct      >  2:358088/1‑141 (MQ=255)
                                                                                                                                      ggTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGAAAgctgc    >  1:143860/1‑141 (MQ=255)
                                                                                                                                        tGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGAAAGctgctg  >  1:421695/1‑141 (MQ=255)
                                                                                                                                        tGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGAAAGctgctg  >  1:334367/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                        
ATTTATTTACGAAATTATCATTAATCAGTGTGTTAGTACTGCACTTAAAACAAGAGAATGTATTAGACCAACCGGGCTTAGAATTTAAATATCCAAAACCGAAACGGAAGTCTAGGTTCTTTATAATTTCAATGGTGCTTGCAGTGACATGTTTTATCGGTTATAACATGTACTTACTTTACAATAATACTATCAATACAAATATCTCCATTATTGGCCATCGTGGTTTCGAAGATAAAGGCGTTGAAAATTCTATTCCGTCATTGAAAGCTGCTG  >  CP000730/264654‑264929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: