Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 494,590 A→G 9.3% I138T (ATT→ACT)  yibF ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730494,5900AG 9.3% 119.7 / 5.1 43I138T (ATT→ACT) yibFhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/18);  new base G (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

GTTCGCAGATGTCGCTAAAATTTCTCTACCAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGATAAATAAATGAGTGT  >  CP000730/494451‑494729
                                                                                                                                           |                                                                                                                                           
gtTCGCAGATGTCGCTAAAATTTCTCTACCAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAAt                                                                                                                                            >  1:66542/1‑141 (MQ=255)
             gCTAAAATTTCTCTACCAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGaata                                                                                                                               >  2:110577/1‑141 (MQ=255)
              cTAAAATTTCTCTACCAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACTGCAAGaataa                                                                                                                              >  1:62460/1‑141 (MQ=255)
                 aaaTTTCTCTACCAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAGTAACAGCAAGAATAATAg                                                                                                                           >  1:226213/1‑141 (MQ=255)
                 aaaTTTCTCTACCAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAGTAACAGCAAGAATAATAg                                                                                                                           >  1:11771/1‑141 (MQ=255)
                          tACCAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACATCTGCAATAACAGCAAGAATAATAGTGAACACCa                                                                                                                  <  1:240418/141‑1 (MQ=255)
                            ccAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATa                                                                                                                >  1:185905/1‑141 (MQ=255)
                              aaCACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATaaa                                                                                                              <  1:63329/141‑1 (MQ=255)
                                        ccAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCata                                                                                                    >  2:115529/1‑141 (MQ=255)
                                        ccAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCata                                                                                                    <  1:222077/141‑1 (MQ=255)
                                        ccAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCata                                                                                                    <  1:193671/141‑1 (MQ=255)
                                        ccAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCata                                                                                                    <  1:408715/141‑1 (MQ=255)
                                          aGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATc                                                                                                  >  1:367323/1‑141 (MQ=255)
                                                   taatTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAat                                                                                         <  2:424879/141‑1 (MQ=255)
                                                   taatTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAat                                                                                         <  2:326279/141‑1 (MQ=255)
                                                     atTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAatat                                                                                       >  2:294079/1‑141 (MQ=255)
                                                      ttCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATAtt                                                                                      <  1:457141/141‑1 (MQ=255)
                                                         gTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCAt                                                                                   <  1:93113/141‑1 (MQ=255)
                                                         gTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCAt                                                                                   <  1:42881/141‑1 (MQ=255)
                                                         gTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCAt                                                                                   >  1:296244/1‑141 (MQ=255)
                                                         gTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCAt                                                                                   <  1:99865/141‑1 (MQ=255)
                                                            ttGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGa                                                                                <  2:446364/141‑1 (MQ=255)
                                                            ttGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCAAATATACATTGa                                                                                <  2:18010/141‑1 (MQ=255)
                                                                  aaGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATacac                                                                          <  2:185905/141‑1 (MQ=255)
                                                                   aGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCGGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATAcaca                                                                         <  1:456090/141‑1 (MQ=255)
                                                                        tGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGt                                                                    >  1:225635/1‑141 (MQ=255)
                                                                            ttCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCa                                                                >  2:88811/1‑141 (MQ=255)
                                                                                cTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCtt                                                            >  2:368587/1‑141 (MQ=255)
                                                                                  tGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCttgt                                                          >  2:297026/1‑141 (MQ=255)
                                                                                  tGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCttgt                                                          <  1:8882/141‑1 (MQ=255)
                                                                                          ttCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGt                                                  >  2:82646/1‑141 (MQ=255)
                                                                                                         aCTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAg                                   >  2:331663/1‑141 (MQ=255)
                                                                                                         aCTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAg                                   >  2:288845/1‑141 (MQ=255)
                                                                                                                 tAATTGTTAAATCTATCACAGCTGCAGTAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGa                           <  2:226213/141‑1 (MQ=255)
                                                                                                                 tAATTGTTAAATCTATCACAGCTGCAGTAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGa                           <  2:11771/141‑1 (MQ=255)
                                                                                                                    ttGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGt                        >  2:247882/1‑141 (MQ=255)
                                                                                                                          aaTCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCg                  >  2:215115/1‑141 (MQ=255)
                                                                                                                             cTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGata               <  1:181632/141‑1 (MQ=255)
                                                                                                                             cTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGata               >  2:385004/1‑141 (MQ=255)
                                                                                                                             cTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAAATGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGata               >  1:401716/1‑141 (MQ=255)
                                                                                                                                tCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGataaat            <  2:296244/141‑1 (MQ=255)
                                                                                                                                  acaGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGataaataa          >  1:162481/1‑141 (MQ=255)
                                                                                                                                   caGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGAtaaataaa         >  1:172824/1‑141 (MQ=255)
                                                                                                                                          aaTAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGATAAATAAATGAgtgt  >  1:248219/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GTTCGCAGATGTCGCTAAAATTTCTCTACCAACACGCATGCCAGATTGGAATAATTCGTATTGCGTAAGCGTTGGATTCACTTGATGCAATTCATAAATGGGTGAACTAATGGTAATTGTTAAATCTATCACAGCTGCAATAACAGCAAGAATAATAGTGAACACCATAAATTGAACCATATCAATGCCAATATTCATTGAATACACATATGTTTCATCTTGTTGTTCGGTTGAAAAGCCTTGTAGATGACCGAAGTAGACCGATAAATAAATGAGTGT  >  CP000730/494451‑494729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: