Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,776,059 A→G 6.7% D155D (GAT→GAC clpX ← S14 family endopeptidase ClpX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,776,0590AG 6.7% 161.1 / 4.9 60D155D (GAT→GACclpXS14 family endopeptidase ClpX
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base G (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

CTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTACCTGTTGGCCCAATTAATGCAAT  >  CP000730/1775920‑1776187
                                                                                                                                           |                                                                                                                                
gtggaacggggcGTGTTATAGATGTGTTTTCAGATTTACGTGCAGTTTTGTCGATGTCATCTACATAGATGATACCTTTTTCGGCTTTATCAATGGCAAAGTCAGCTGCTTGAATTAATCTCAAGAAGATATTTTCAAcat                                                                                                                                 <  2:348293/130‑1 (MQ=255)
cTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAAcat                                                                                                                                 <  2:108354/141‑1 (MQ=255)
 tGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAAcatc                                                                                                                                >  1:174249/1‑141 (MQ=255)
     aCGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCg                                                                                                                            >  2:386458/1‑141 (MQ=255)
             tgtTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATa                                                                                                                    <  2:116308/141‑1 (MQ=255)
             tgtTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATa                                                                                                                    >  2:444533/1‑141 (MQ=255)
             tgtTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATa                                                                                                                    <  1:231504/141‑1 (MQ=255)
             tgtTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATa                                                                                                                    >  1:311770/1‑141 (MQ=255)
                     aTGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACGTCATCGCCTACATAACCAGCtt                                                                                                            >  2:385568/1‑141 (MQ=255)
                     aTGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACGTCATCGCCTACATAACCAGCtt                                                                                                            >  2:244379/1‑141 (MQ=255)
                     aTGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCtt                                                                                                            >  1:380861/1‑141 (MQ=255)
                     aTGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCtt                                                                                                            >  2:447760/1‑141 (MQ=255)
                     aTGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCtt                                                                                                            >  2:347193/1‑141 (MQ=255)
                       gtgtTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCGGCGTCa                                                                                                          <  1:129476/141‑1 (MQ=255)
                            ttCAGATTTACGTGCAATTTTATCAATTTCATCTACACAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTaa                                                                                                     >  1:332213/1‑141 (MQ=255)
                             tCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTaaa                                                                                                    >  1:50558/1‑141 (MQ=255)
                             tCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGCCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTaaa                                                                                                    >  1:355876/1‑141 (MQ=255)
                                gATTTACGTGCAATTTNATCAATTTCATCNACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACtt                                                                                                 >  1:258552/1‑141 (MQ=255)
                                  tttACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGt                                                                                               <  1:26848/141‑1 (MQ=255)
                                     aCGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGc                                                                                            >  1:176678/1‑141 (MQ=255)
                                      cGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTTTTTTTTTTCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCa                                                                                           >  2:401715/1‑141 (MQ=255)
                                        tGCAATTTTATCAATTTCATCTACATAAATAATACTTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATc                                                                                         <  1:63755/141‑1 (MQ=255)
                                        tGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATc                                                                                         >  2:29430/1‑141 (MQ=255)
                                            aTTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCATTTAAACTTGTCGCATCTGCa                                                                                     <  2:191493/141‑1 (MQ=255)
                                                           tCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACa                                                                      <  1:361364/141‑1 (MQ=255)
                                                               cATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCa                                                                  <  1:274790/141‑1 (MQ=255)
                                                                atgaataatACCTTTTTCGGCTTTATCAATGGCAAAGTCAGCTGCTGGAATTAATCTCAACCAGATATGTTCAACATCATCGCCTACATAACCAACTCCAGTTAAACGTGTCGCATCTGCAACAGCAAATGCTACATTCaa                                                                 <  2:166056/138‑1 (MQ=255)
                                                                ataaataaTACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCaa                                                                 <  2:197828/141‑1 (MQ=255)
                                                                ataaataaTACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCGTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCaa                                                                 <  2:450515/141‑1 (MQ=255)
                                                                 taaataaTACCTTTTTCTTCTTTATTAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAAc                                                                >  2:393331/1‑141 (MQ=255)
                                                                  aaataaTACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACg                                                               <  2:329413/141‑1 (MQ=255)
                                                                  aaataaTACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACg                                                               <  2:61017/141‑1 (MQ=255)
                                                                       ataCCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTg                                                          >  2:147415/1‑141 (MQ=255)
                                                                            tttttCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTaa                                                     <  1:41526/141‑1 (MQ=255)
                                                                             ttttCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAg                                                    <  2:52480/141‑1 (MQ=255)
                                                                             ttttCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAg                                                    <  2:294004/141‑1 (MQ=255)
                                                                             ttttCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAg                                                    <  2:78869/141‑1 (MQ=255)
                                                                                tttttttttttaaTGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATTGTACATTCAACGTCTTGGCTAAGGtt                                                 >  2:97972/12‑141 (MQ=255)
                                                                                tctttttttTCAATGTCAAAGACAGCTGCTTGAATTAATTTCAACAATATATTTACAACATCATCGACTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTTCAAATTATACATTCAACGTCTTTGCTAAGttt                                                 >  2:230277/6‑141 (MQ=255)
                                                                                tCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGtt                                                 >  2:165672/1‑141 (MQ=255)
                                                                                tCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGtt                                                 >  2:96338/1‑141 (MQ=255)
                                                                                 cGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGttt                                                <  2:258552/141‑1 (MQ=255)
                                                                                     tttATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCCAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGc                                            <  2:327437/141‑1 (MQ=255)
                                                                                     tttATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGc                                            <  2:206572/141‑1 (MQ=255)
                                                                                      ttATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCt                                           <  2:333364/141‑1 (MQ=255)
                                                                                        aTCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAATATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCtaa                                         <  1:363178/141‑1 (MQ=255)
                                                                                        aTCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCtaa                                         >  2:287613/1‑141 (MQ=255)
                                                                                        aTCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCtaa                                         >  2:50032/1‑141 (MQ=255)
                                                                                        aTCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCtaa                                         <  1:302888/141‑1 (MQ=255)
                                                                                         tCAATGTCAAAGTCAGCTGCTTGAATTATTCTCAACATGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCtaat                                        <  1:215855/141‑1 (MQ=255)
                                                                                         tCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCCACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCtaat                                        <  1:44974/141‑1 (MQ=255)
                                                                                         tCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCtaat                                        <  1:423326/141‑1 (MQ=255)
                                                                                                   aggcAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTAc                              <  2:176678/138‑1 (MQ=255)
                                                                                                      cAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCAc                           >  1:198166/1‑141 (MQ=255)
                                                                                                      cAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCAc                           >  1:253942/1‑141 (MQ=255)
                                                                                                         ctgctTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTAc                        <  2:350378/141‑1 (MQ=255)
                                                                                                         ctgctTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTAc                        <  2:302043/141‑1 (MQ=255)
                                                                                                                 aTTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTACCTGTTGGc                >  2:251954/1‑141 (MQ=255)
                                                                                                                    aaTCTCAACAAGATATTTTCAACGTCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTACCTGTTGGCCCa             <  1:244379/141‑1 (MQ=255)
                                                                                                                    aaTCTCAACAAGATATTTTCAACGTCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTACCTGTTGGCCCa             <  1:385568/141‑1 (MQ=255)
                                                                                                                     aTCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTACCTGTTGGCCCaa            <  1:441890/141‑1 (MQ=255)
                                                                                                                             aaGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTACCTGTTGGCCCAATTAATGCa    >  2:225963/1‑141 (MQ=255)
                                                                                                                               gATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTACCTGTTGGCCCAATTAATGCAAt  >  1:306335/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                
CTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAACATCATCGCCTACATAACCAGCTTCAGTTAAACTTGTCGCATCTGCAATTGCAAATGGTACATTCAACGTCTTGGCTAAGGTTTGAGCTAATAATGTTTTACCACTACCTGTTGGCCCAATTAATGCAAT  >  CP000730/1775920‑1776187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: