Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,101,143 A→G 5.5% V268A (GTA→GCA)  USA300HOU_1959 ← bacteriophage tail length tape measure protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,101,1430AG 5.5% 228.4 / 3.7 72V268A (GTA→GCA) USA300HOU_1959bacteriophage tail length tape measure protein
Reads supporting (aligned to +/- strand):  ref base A (39/29);  new base G (2/2);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

CGCCGTATTTAGTAATACTATCAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAG  >  CP000730/2101003‑2101272
                                                                                                                                            |                                                                                                                                 
cgcCGTATTTAGTAATACTATCAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTa                                                                                                                                   >  2:418597/1‑141 (MQ=255)
cgcCGTATTTAGTAATACTATCAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTa                                                                                                                                   >  2:259330/1‑141 (MQ=255)
           gTAATACTATCAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCATGGGTAATTAACTGTACGGCTTGcaca                                                                                                                        >  1:257640/1‑141 (MQ=255)
               tACTATCAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTGCGGCTTGCACACCGt                                                                                                                    >  1:131773/1‑141 (MQ=255)
               tACTATCAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTGCGGCTTGCACACCGt                                                                                                                    >  1:6971/1‑141 (MQ=255)
               tACTATCAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGt                                                                                                                    >  2:50107/1‑141 (MQ=255)
                     cAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAAc                                                                                                              <  2:236716/141‑1 (MQ=255)
                     cAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAAc                                                                                                              <  1:246496/141‑1 (MQ=255)
                       gCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCt                                                                                                            >  2:260854/1‑141 (MQ=255)
                       gCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCt                                                                                                            >  2:171416/1‑141 (MQ=255)
                       gCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCt                                                                                                            >  2:140429/1‑141 (MQ=255)
                          aaTGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGtt                                                                                                         >  2:246898/1‑141 (MQ=255)
                           aTGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACATTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTta                                                                                                        >  1:37117/1‑141 (MQ=255)
                           aTGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTta                                                                                                        >  2:426116/1‑141 (MQ=255)
                           aTGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTta                                                                                                        >  2:426084/1‑141 (MQ=255)
                             gtgtCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTtata                                                                                                      >  1:87572/1‑141 (MQ=255)
                             gtgtCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTtata                                                                                                      >  1:456392/1‑141 (MQ=255)
                              agtCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACAATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTTAACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTatat                                                                                                     >  2:370296/2‑141 (MQ=255)
                               gtCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATg                                                                                                    >  1:424274/1‑141 (MQ=255)
                                tCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGa                                                                                                   >  1:128747/1‑141 (MQ=255)
                                tCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGa                                                                                                   >  1:333950/1‑141 (MQ=255)
                                           cccACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCaa                                                                                        >  1:30984/1‑141 (MQ=255)
                                           cccACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCaa                                                                                        >  2:155122/1‑141 (MQ=255)
                                           cccACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCaa                                                                                        >  1:420092/1‑141 (MQ=255)
                                                   cTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGAct                                                                                >  2:4880/1‑141 (MQ=255)
                                                    ttGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGActc                                                                               <  1:209611/141‑1 (MQ=255)
                                                    ttGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGActc                                                                               <  2:73570/141‑1 (MQ=255)
                                                    ttGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGActc                                                                               <  1:287010/141‑1 (MQ=255)
                                                      gcgcCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCtg                                                                             >  1:419526/1‑141 (MQ=255)
                                                         ccgcTTTTGCTACCATATCCAATACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTgg                                                                          <  2:176789/141‑1 (MQ=255)
                                                         ccgcTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTgg                                                                          <  2:295636/141‑1 (MQ=255)
                                                         ccgcTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTgg                                                                          <  2:369498/141‑1 (MQ=255)
                                                           gcTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCa                                                                        >  1:313320/1‑141 (MQ=255)
                                                           gcTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCa                                                                        >  1:136030/1‑141 (MQ=255)
                                                           gcTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCa                                                                        >  1:199334/1‑141 (MQ=255)
                                                            cTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCAt                                                                       <  1:260854/141‑1 (MQ=255)
                                                              tttGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCAttt                                                                     <  2:424274/141‑1 (MQ=255)
                                                               ttGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCAtttt                                                                    <  2:389646/141‑1 (MQ=255)
                                                                tGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTc                                                                   >  2:224060/1‑141 (MQ=255)
                                                                    aCCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGt                                                               >  1:293967/1‑141 (MQ=255)
                                                                    aCCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGt                                                               >  1:225449/1‑141 (MQ=255)
                                                                        tatCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCtt                                                           <  2:326003/141‑1 (MQ=255)
                                                                             aaaaCACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCt                                                      <  1:31968/141‑1 (MQ=255)
                                                                              aaaCACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTg                                                     >  2:218933/1‑141 (MQ=255)
                                                                                      tGATATTCACTTGCTTCGATATCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCt                                             <  2:187982/141‑1 (MQ=255)
                                                                                      tGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCt                                             <  2:444528/141‑1 (MQ=255)
                                                                                       gATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTa                                            >  2:266260/1‑141 (MQ=255)
                                                                                        atatTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTaa                                           >  1:184896/1‑141 (MQ=255)
                                                                                        atatTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTaa                                           >  1:257897/1‑141 (MQ=255)
                                                                                           ttCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCt                                        <  1:418597/141‑1 (MQ=255)
                                                                                           ttCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCt                                        <  1:259330/141‑1 (MQ=255)
                                                                                               cTTGCTTCGATTCCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTa                                    <  1:323105/141‑1 (MQ=255)
                                                                                                ttGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTAt                                   >  2:200071/1‑141 (MQ=255)
                                                                                                 tgctgcGATCCCTGCATCGCCCATTGCACGAGTAATTAACTGTACGGCTTGCACACCGTCTTAACCTGTTATCTGACTAAATTTCAAGAATCACTCTGTGGCATTTTCACGTTCTTTACCTGTAAAACCTAATCTTGTAtt                                  <  2:51536/141‑1 (MQ=255)
                                                                                                           ccTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCt                        <  1:47182/141‑1 (MQ=255)
                                                                                                           ccTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCt                        <  2:298185/141‑1 (MQ=255)
                                                                                                           ccTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCt                        >  1:82599/1‑141 (MQ=255)
                                                                                                           ccTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCt                        <  2:1332/141‑1 (MQ=255)
                                                                                                           ccTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCt                        <  2:429753/141‑1 (MQ=255)
                                                                                                           ccTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGGTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCt                        >  1:355348/1‑141 (MQ=255)
                                                                                                             tGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTaa                      >  2:79804/1‑141 (MQ=255)
                                                                                                             tGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTaa                      >  2:455076/1‑141 (MQ=255)
                                                                                                             tGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTaa                      >  1:210291/1‑141 (MQ=255)
                                                                                                                      cATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTccacc             >  1:376387/1‑141 (MQ=255)
                                                                                                                       aTTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTccacca            <  2:128747/141‑1 (MQ=255)
                                                                                                                        ttGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCaa           >  1:455733/1‑141 (MQ=255)
                                                                                                                            aCGGGTAATTAACTGTGCGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGt       <  2:6971/141‑1 (MQ=255)
                                                                                                                            aCGGGTAATTAACTGTGCGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGt       <  2:131773/141‑1 (MQ=255)
                                                                                                                            aCGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGt       >  2:207477/1‑141 (MQ=255)
                                                                                                                            aCGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGt       <  1:50107/141‑1 (MQ=255)
                                                                                                                                gTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGACTGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCa   <  1:135189/141‑1 (MQ=255)
                                                                                                                                 tAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAg  <  1:426084/141‑1 (MQ=255)
                                                                                                                                 tAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAg  <  1:426116/141‑1 (MQ=255)
                                                                                                                                 tAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAg  <  2:37117/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                 
CGCCGTATTTAGTAATACTATCAGCTAATGTGTCAACACTTATCCCACTAGCTTGCGCCGCTTTTGCTACCATATCCAAAACACTTTGATATTCACTTGCTTCGATACCTGCATCGCCCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAG  >  CP000730/2101003‑2101272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: