Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,306,605 G→T 33.3% G223V (GGA→GTA)  fabG → short chain dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,306,6050GT33.3% 16.5 / 5.2 12G223V (GGA→GTA) fabGshort chain dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base T (2/2);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAGGGGTGAGGT  >  CP000730/1306472‑1306741
                                                                                                                                     |                                                                                                                                        
atGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACtata                                                                                                                                   <  2:48980/141‑1 (MQ=255)
               cAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGt                                                                                                                    >  2:124204/1‑141 (MQ=255)
                                 gCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAat                                                                                                  >  2:98775/1‑141 (MQ=255)
                                  cAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGTAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAata                                                                                                 >  2:99904/1‑141 (MQ=255)
                                  cAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGTAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAata                                                                                                 >  2:99896/1‑141 (MQ=255)
                                                           gCGATTGGTTTTACCGAGTGAGGTTGCTCATACAAGCGCCTATTTATATCACCCAAAAGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAAAGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGt                                                                        >  2:7922/1‑141 (MQ=255)
                                                                           aGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGtt                                                        >  2:118417/1‑141 (MQ=255)
                                                                                                                   aTGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGttaattaa                >  1:78202/1‑141 (MQ=255)
                                                                                                                   aTGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGttaattaa                >  1:78210/1‑141 (MQ=255)
                                                                                                                                 aCTGTAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAGGGGTGAGGt  <  1:99896/141‑1 (MQ=255)
                                                                                                                                 aCTGTAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAGGGGTGAGGt  <  1:99904/141‑1 (MQ=255)
                                                                                                                                 aCTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAGGGGTGAGGt  <  1:96272/141‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
ATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAGGGGTGAGGT  >  CP000730/1306472‑1306741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: