Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,010,695 T→A 33.5% L35L (CTA→CTT ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,010,6950TA33.5% 7.0 / 9.8 12L35L (CTA→CTTygaDABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base T (5/3);  new base A (2/2);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

TTCAATTGGTGGTCTCACTATTACAAAAATAAATAATGCGATACCAATGGCAATAGTTAAATGATGAACTTTTTCATCAGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAATAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCAAAAATACGATATTTGTATGGCTTAACAAATTGCAAATATCGTTTAATCATACAATCCCCCCTAATCTATTGCCCTATCCTATTC  >  CP000730/2010566‑2010833
                                                                                                                                 |                                                                                                                                          
ttCAATTGGTGGTCTCACTATTACAAAAATAAATAATGCGATACCAATGGCAATAGTTAAATGATGAACTTTTTCATCAGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAATAGTGGTATAAg                                                                                                                                 >  2:89149/1‑141 (MQ=255)
        gtggtCTCACTATTACAAAAATAAATAATGCGATACCAATGGCAATAGTTAAATGATGAACTTTTTCATCAGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAATAGTGGTATAAGCATTGGta                                                                                                                         >  1:115996/1‑141 (MQ=255)
          ggtCTCACTATTACAAAAATAAATAATGCGATACCAATGGCAATAGTTAAATGATGAACTTTTTCATCAGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAAAAGTGGTATAAGCATTGGtata                                                                                                                       >  2:65440/1‑141 (MQ=255)
          ggtCTCACTATTACAAAAATAAATAATGCGATACCAATGGCAATAGTTAAATGATGAACTTTTTCATCAGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAAAAGTGGTATAAGCATTGGtata                                                                                                                       >  2:102954/1‑141 (MQ=255)
              tcACTATTACAAAAATAAATAATGCGATACCAATGGCAATAGTTAAATGATGAACTTTTTCATCAGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAATAGTGGTATAAGCATTGGTATACCaa                                                                                                                   >  2:77727/1‑141 (MQ=255)
                                           ccAATGGCAATAGTTAAATGATGAACTTTTTCATCAGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAATAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCa                                                                                      <  2:100791/141‑1 (MQ=255)
                                                                            tcaGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAATAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCAAAAATACGATATTTGTATGGCTTAACAAATTGc                                                     >  1:81200/1‑141 (MQ=255)
                                                                                      gTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAAAAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCAAAAATACGATATTTATATGGCTTAACAAATTGCAAATATCGtt                                           <  1:102954/141‑1 (MQ=255)
                                                                                      gTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAAAAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCAAAAATACGATATTTATATGGCTTAACAAATTGCAAATATCGtt                                           <  1:65440/141‑1 (MQ=255)
                                                                                                          ccATCTATTGCATATTTAATTAATAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCAAAAATACGATATTTGTATGGCTTAACAAATTGCAAATATCGTTTAATCATACAATCCCCCCTa                       >  2:16098/1‑141 (MQ=255)
                                                                                                           cATCTATTGCATATTTAATTAATAGTGGTATAAGCATTGGTATACCAAACTTATTTATCCCAACAATAATCGTTGCAAATATACGATATTTGTATGGCTTAACAAATTGCAAATATCGTTTAATCATACAATCCCCCCTaa                      <  2:115996/141‑1 (MQ=255)
                                                                                                                aTTGCATATTTAATTAATAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCAAAAATACGATATTTGTATGGCTTAACAAATTGCAAATATCGTTTAATCATACAATCCCCCCTAATCTAt                 <  1:77727/141‑1 (MQ=255)
                                                                                                                               aatAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCAAAAATACGATATTTGTATGGCTTAACAAATTGCAAATATCGTTTAATCATACAATCCCCCCTAATCTATTGCCCTATCCTATTc  >  1:40167/1‑141 (MQ=255)
                                                                                                                                 |                                                                                                                                          
TTCAATTGGTGGTCTCACTATTACAAAAATAAATAATGCGATACCAATGGCAATAGTTAAATGATGAACTTTTTCATCAGTCGTTAGTGCGTGGTTATTAATCACGCCATCTATTGCATATTTAATTAATAGTGGTATAAGCATTGGTATACCAAACTTAATTATCCCAACAATAATCGTTGCAAAAATACGATATTTGTATGGCTTAACAAATTGCAAATATCGTTTAATCATACAATCCCCCCTAATCTATTGCCCTATCCTATTC  >  CP000730/2010566‑2010833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: