Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,139,513 A→T 36.4% I16I (ATT→ATA xtmA ← terminase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,139,5130AT36.4% 5.0 / 9.3 11I16I (ATT→ATAxtmAterminase small subunit
Reads supporting (aligned to +/- strand):  ref base A (2/5);  new base T (2/2);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTTTTGTTCTTGTATATATTGCTTGATGTGCGGCTTTTTCAATAACCTACACGCTGTCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTGCAGTTAACTCACTCATTTTATCACCCCCACAATTTTATCTAATATGGTTTCATACC  >  CP000730/2139390‑2139600
                                                                                                                           |                                                                                       
atcttTTTGTTCTTGTNTATATTGCTTGANGTGTGGCTTCTTTAATAACCTACACCCTGTCACATGTGCACTATTTGCGCTATAACCTGCTTTTATGGCACTTTGTGTCACATTAAGTGTTCTTATATACTCATTCACaaa                                                                        >  1:114420/1‑141 (MQ=255)
atcttTTTGTTCTTGTATATATTGCTTGATGTGTGGCTTCTTTAATAACCTACACCCTGTCACATGTGCACTATTTGCGCTATAACCTGCTTTTATGGCACTTTGTGTCACATTAAGTGTTCTTATATACTCATTCACaaa                                                                        >  1:9094/1‑141 (MQ=255)
              gTATATATTGCTTGATGTGCGGCTTTTTCAATAACCTACACGCTGTCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTg                                                          <  2:28553/141‑1 (MQ=255)
                        cTTGATGTGCGGCTTTTTCAATAACCTACACGCTGTCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTGCAGTTAactc                                                <  1:5476/141‑1 (MQ=255)
                        cTTGATGTGCGGCTTTTTCAATAACCTACACGCTGTCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTGCAGTTAactc                                                <  2:43617/141‑1 (MQ=255)
                                          cAATAACCTACACGCTGTCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTGCAGTTAACTCACTCATTTTATCAccccc                              <  2:112308/141‑1 (MQ=255)
                                              aaCCTACACGCTGTCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTGCAGTTAACTCACTCATTTTATCACCCCCACaa                          <  2:100901/141‑1 (MQ=255)
                                                   acacGCTGTCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTGCAGTTAACTCACTCATTTTATCACCCCCACAATTTTa                     >  1:73958/1‑141 (MQ=255)
                                                           tCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTGCAGTTAACTCACTCATTTTATCACCCCCACAATTTTATCTAatat             >  2:43218/1‑141 (MQ=255)
                                                                      cTATTTGCGCTATAACCTGCTTTTATGGCACTTTGTGTCACATTAAGTGTTCTTATATACTCATTCACAAAACGCGCTTGCTTTGCCGTTAACTCACTCATTCTATCACCTCCACAATTTTATCTAATAAGGTTTCATAcc  <  2:114420/141‑1 (MQ=255)
                                                                      cTATTTGCGCTATAACCTGCTTTTATGGCACTTTGTGTCACATTAAGTGTTCTTATATACTCATTCACAAAACGCGCTTGCTTTGCCGTTAACTCACTCATTCTATCACCTCCACAATTTTATCTAATAAGGTTTCATAcc  <  2:9094/141‑1 (MQ=255)
                                                                                                                           |                                                                                       
ATCTTTTTGTTCTTGTATATATTGCTTGATGTGCGGCTTTTTCAATAACCTACACGCTGTCACATGTGCGCTATTTGCGTTATATCCTGCTTTTATGGCACTTTGTGTTACATTCAGTGTTCTAATATACTCATTCACAAAACGTGCTTGCTTTGCAGTTAACTCACTCATTTTATCACCCCCACAATTTTATCTAATATGGTTTCATACC  >  CP000730/2139390‑2139600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: