Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 512,643 A→G 58.8% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730512,6430AG58.8% 4.8 / 16.0 17intergenic (+711/‑150)recR/rrs1recombination protein RecR/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base G (6/4);  total (9/8)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

GTGTAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAAT  >  CP000730/512505‑512771
                                                                                                                                          |                                                                                                                                
gtgtAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTtaat                                                                                                                                >  2:105738/1‑141 (MQ=255)
                    gtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCtt                                                                                                            <  1:13120‑M1/46‑1 (MQ=255)
                                        cgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACa                                                                                        <  2:12073‑M1/66‑1 (MQ=255)
                                                         ggCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACa                                                                       <  1:105738/141‑1 (MQ=255)
                                                            ttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAata                                                                    >  1:11394‑M1/56‑141 (MQ=255)
                                                                              tAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCaa                                                  <  1:89018/141‑1 (MQ=255)
                                                                                    aTAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACg                                            <  2:56388/141‑1 (MQ=255)
                                                                                           aaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACtat                                     >  1:63267‑M1/25‑141 (MQ=255)
                                                                                                             aattTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTAttt                   >  2:32595/1‑141 (MQ=255)
                                                                                                               ttttAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAg                 <  2:5757/141‑1 (MQ=255)
                                                                                                               ataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGTTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTACAAAAAACGTAACTATAAGTTACAAACATTATTTAg                 >  2:104565‑M1/5‑141 (MQ=255)
                                                                                                               ataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAg                 <  2:46978‑M1/137‑1 (MQ=255)
                                                                                                                taaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGt                >  2:13120‑M1/4‑141 (MQ=255)
                                                                                                                        gTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGa        >  2:57027/1‑141 (MQ=255)
                                                                                                                         ttATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTAATGAg       >  1:68486/1‑141 (MQ=255)
                                                                                                                          tATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGc      >  1:38896/1‑141 (MQ=255)
                                                                                                                          tATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGc      >  1:16926/1‑141 (MQ=255)
                                                                                                                              gACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAAt  <  1:26848/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                
GTGTAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAAT  >  CP000730/512505‑512771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: