| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,793,181 | T→A | 28.6% | S222S (TCA→TCT) | polA ← | DNA‑directed DNA polymerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,793,181 | 0 | T | A | 28.6% | 21.2 / 9.2 | 14 | S222S (TCA→TCT) | polA | DNA‑directed DNA polymerase I |
| Reads supporting (aligned to +/- strand): ref base T (4/6); new base A (2/2); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCTTGATGAGTCATTAACGTATCTTCAAGTTTTACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCTGTTTTCTCTCCAACACCTGCAACACCTGGTATATTATCAG > CP000730/1793051‑1793287 | cATCTTGATGAGTCATTAACGTATCTTCAAGTTTTACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCAGAAATTTCAt > 2:12767/1‑141 (MQ=255)cATCTTGATGAGTCATTAACGTATCTTCAAGCTTTACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCAGAAATTTCAt > 2:84197/1‑141 (MQ=255) ttGATGAGTCATTAACGTATCTTCAAGTTTTACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTaa < 2:108191/141‑1 (MQ=255) tAACGTATCTTCAAGTTTTACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGac < 1:62081/141‑1 (MQ=255) aaGTTTTACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACtgt > 2:87535/1‑141 (MQ=255) aaGTTTTACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACtgt > 2:28636/1‑141 (MQ=255) tACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCaaa < 2:4495/141‑1 (MQ=255) tAATCTTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATATACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCtt > 2:29736/1‑140 (MQ=255) tAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCAGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCTGTTTTCTCTcc < 1:12767/141‑1 (MQ=255) tAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCAGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCTGTTTTCTCTcc < 1:84197/141‑1 (MQ=255) cTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCTGTTTTCTCTCcaacacctgca < 1:26218/141‑1 (MQ=255) tAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCTGTTTTCTCTCcaacacctgcaacacc < 1:28636/141‑1 (MQ=255) tAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCTGTTTTCTCTCcaacacctgcaacacc < 1:87535/141‑1 (MQ=255) tGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCTGTTTTCTCTCCAACACCTGCAACACCTGGTATATTATCAg > 1:95482/1‑141 (MQ=255) | CATCTTGATGAGTCATTAACGTATCTTCAAGTTTTACTTCAATCGGACTATCTACATTAATCGTTGCTAATTCTTTACTCATTAATGCATCTTCTTTGCTATTTTGAAGTTTTTCTTTTAACTTTTTACCTGAAATTTCATCTAAATGTTCATAGACACCTTCTACTGTGTCAAATTGGTTTAGCAATTTTATTGCTGTTTTCTCTCCAACACCTGCAACACCTGGTATATTATCAG > CP000730/1793051‑1793287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |