Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,459,907 T→C 13.8% K21R (AAA→AGA)  USA300HOU_1356 ← possible acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,459,9070TC13.8% 68.7 / 6.0 29K21R (AAA→AGA) USA300HOU_1356possible acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (14/11);  new base C (2/2);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

GTCGACAACAATAAAGCCATAAATTTTGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACATGCTT  >  CP000730/1459768‑1460043
                                                                                                                                           |                                                                                                                                        
gtcGACAACAATAAAGCCATAAATTTTGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCttt                                                                                                                                         <  2:34314/141‑1 (MQ=255)
   gACAACAATAGAGCCATAAATTTGGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAGATGTTCTAGGAGTGGGTACTGATCGTCCCGTTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGc                                                                                                                                      <  2:78950/141‑1 (MQ=255)
   gACAACAATAAAGCCATAAATTTTGTCATTTTCCTCTAATAGGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCTATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGc                                                                                                                                      <  2:214595/141‑1 (MQ=255)
   gACAACAATAAAGCCATAAATTTTGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGc                                                                                                                                      <  1:101927/141‑1 (MQ=255)
   gACAACAATAAAGCCATAAATTTTGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGc                                                                                                                                      <  2:29441/141‑1 (MQ=255)
   gACAACAATAAAGCCATAAATTTTGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGc                                                                                                                                      <  2:248920/141‑1 (MQ=255)
   gACAACAATAAAGCCATAAATTTTGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGc                                                                                                                                      <  2:29422/141‑1 (MQ=255)
                    aaaTTTTGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTa                                                                                                                     >  2:222441/1‑141 (MQ=255)
                          tGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGAttt                                                                                                               <  2:207053/141‑1 (MQ=255)
                             cATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGGTTTCAGATTTGAt                                                                                                            >  2:66990/1‑141 (MQ=255)
                                     tctAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCt                                                                                                    >  1:162495/1‑141 (MQ=255)
                                              tACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTa                                                                                           >  2:69653/1‑141 (MQ=255)
                                              tACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTa                                                                                           >  2:68933/1‑141 (MQ=255)
                                                 aaaTAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTCTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCt                                                                                        >  1:151800/1‑141 (MQ=255)
                                                 aaaTAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTCTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCt                                                                                        >  1:151802/1‑141 (MQ=255)
                                                   ataatCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAg                                                                                      >  2:238387/1‑141 (MQ=255)
                                                      atCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACg                                                                                   <  1:98762/141‑1 (MQ=255)
                                                       tCTTTAGCAATCTCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACgg                                                                                  <  2:228508/141‑1 (MQ=255)
                                                                             aaTGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTtata                                                            <  2:248252/141‑1 (MQ=255)
                                                                                               aCTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGt                                          >  1:94731/1‑141 (MQ=255)
                                                                                                                  gTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTTAACATGTTTTTCAGTACATGTTAAtt                       >  1:149105/1‑140 (MQ=255)
                                                                                                                  gTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATc                       >  2:185588/1‑141 (MQ=255)
                                                                                                                   ttGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCt                      >  2:112021/1‑141 (MQ=255)
                                                                                                                                   ttAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACAt      >  1:147647/1‑141 (MQ=255)
                                                                                                                                   ttAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACAt      >  1:163690/1‑141 (MQ=255)
                                                                                                                                   ttAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACAt      >  1:147640/1‑141 (MQ=255)
                                                                                                                                   ttAATTCTCTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACAt      <  2:151802/141‑1 (MQ=255)
                                                                                                                                   ttAATTCTCTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACAt      <  2:151800/141‑1 (MQ=255)
                                                                                                                                     aaTTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACATGc    <  1:238387/141‑1 (MQ=255)
                                                                                                                                       ttCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACATGCtt  >  1:82915/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GTCGACAACAATAAAGCCATAAATTTTGTCATTTTCCTCTAATACGTACAAATAATCTTTAGCAATATCTTCTTCAAAATGTTCTAAAAGTGGGTACTGATCGTCCCATTGCTCGTTGTCGTGTTCTTTCATTAATTCTTTTGCTTCTTCTACTAGATTTAAGATTTGATCTAAATCTGACATTTTACCTAGACGGATCATGTACGTCACTCCTTATATCATTATGTTAAACATGTTTTTCAGTACATGTTAATCTTACACACTATTAACATGCTT  >  CP000730/1459768‑1460043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑