| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,138,686 | A→T | 14.5% | intergenic (‑34/+284) | USA300HOU_2020 ← / ← xtmA | hypothetical membrane protein/terminase small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,138,686 | 0 | A | T | 14.5% | 73.8 / 4.7 | 26 | intergenic (‑34/+284) | USA300HOU_2020/xtmA | hypothetical membrane protein/terminase small subunit |
| Reads supporting (aligned to +/- strand): ref base A (15/7); new base T (2/2); total (17/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCATCCGAAAGCAATAAATTCAAACTAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTGAAATGTTGATGTAATTTGAAAAAAATATCATAC > CP000730/2138569‑2138818 | atcatcCGAAAGCAATAAATTCAAACTAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCTATTGTTTACAAAACACGAAAttt > 2:230990/1‑141 (MQ=255)atcatcCGAAAGCAATAAATTCAAACTAATGCATTGTTTATGACCCATCGAATTTATTGACCAAATAGCTAGAGAAACAAACAACCCAAAATTGAAGATAAATGATATAGTAATAGCTATGGTTTACATAACACGAAAttt > 2:230991/1‑141 (MQ=255) catcCGAAAGCAATAAATTCAAACTAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAAttttt > 1:78486/1‑141 (MQ=255) catcCGAAAGCAATAAATTCAAACTAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAAttttt > 1:144628/1‑141 (MQ=255) aTAAATTCAAACTAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTtttattta > 2:102954/1‑141 (MQ=255) aTAAATTCAAACTAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTtttattta > 1:69985/1‑141 (MQ=255) tCAAACTAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTAtattat > 1:208998/1‑141 (MQ=255) tAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCAttt > 2:85547/1‑141 (MQ=255) taCCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCtt < 2:227235/141‑1 (MQ=255) aTTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCAtttt > 2:166195/1‑141 (MQ=255) aTTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCAtttt > 2:132978/1‑141 (MQ=255) tttATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTtta > 1:23864/1‑141 (MQ=255) aaTAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTAc > 2:230151/1‑141 (MQ=255) aTAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACa > 2:21333/1‑141 (MQ=255) aGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACaat < 1:195686/141‑1 (MQ=255) agaAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTTTTTTTTTTTTTTATTATATATTACAATaatcaa > 2:146682/1‑141 (MQ=255) aaaTAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATaatcaatc < 1:110059/141‑1 (MQ=255) aTAAACACCCCAACATTTAAGATAAGTGATATAGTAATAGCTATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTg < 1:230991/141‑1 (MQ=255) aTAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCTATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTg < 1:230990/141‑1 (MQ=255) acCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTGAAATGtt < 1:241715/141‑1 (MQ=255) cccAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTGAAATGTTg < 1:85547/141‑1 (MQ=255) aaatGATATAGTAATAGCAATTGTTTACAAAACACGAAATGTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTGAAATGTTGATGTAATTTGaaaaa > 2:227859/1‑141 (MQ=255) gATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATTATTTTATTATATATTACAATAATCAATCTGAAATGTTGATGTAATTTGAAAAAAata > 2:37849/1‑141 (MQ=255) gATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTGAAATGTTGATGTAATTTGAAAAAAata > 1:57494/1‑141 (MQ=255) tataGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTGAAATGTTGATGTAATTTGAAAAAAATATc < 1:59050/141‑1 (MQ=255) gTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTGAAATGTTGAAAAAAAAAAAAAAAAATATCATAc < 2:95027/141‑1 (MQ=255) | ATCATCCGAAAGCAATAAATTCAAACTAATGCATTGTTTATTACCCATCGAATTTATTGACCAAATAGCTAGAGAAATAAACAACCCAAAATTTAAAATAAATGATATAGTAATAGCAATTGTTTACAAAACACGAAATTTTTCATTTTTATTTATATTATCCATTTTTCTCCCTTTTTCTTAAATCATTTTATTATATATTACAATAATCAATCTGAAATGTTGATGTAATTTGAAAAAAATATCATAC > CP000730/2138569‑2138818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |