| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,391,574 | A→T | 16.0% | A56A (GCT→GCA) | moaE ← | molybdopterin synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,391,574 | 0 | A | T | 16.0% | 60.5 / 7.2 | 25 | A56A (GCT→GCA) | moaE | molybdopterin synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base A (9/12); new base T (2/2); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
ACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCACGATATTGTTCTGTTTGTAT > CP000730/2391439‑2391711 | aCGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTGGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTGCAATTTGTGCCAATTTCTTTTCAGCCAt > 2:192890/1‑141 (MQ=255) cGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCAtt > 1:50719/1‑141 (MQ=255) cGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCAtt > 1:186618/1‑141 (MQ=255) tGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTT‑TGCCATTGGaa > 2:58757/1‑141 (MQ=255) tGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTT‑TGCCATTGGaa > 2:51741/1‑141 (MQ=255) ggTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAata < 2:76510/141‑1 (MQ=255) ggTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAata < 2:122740/141‑1 (MQ=255) tGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAAtata < 2:178871/141‑1 (MQ=255) aTACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAat < 2:262293/141‑1 (MQ=255) atCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGtttt > 1:213095/1‑141 (MQ=255) gTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAg < 1:39806/141‑1 (MQ=255) aTGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTcc > 1:54567/1‑141 (MQ=255) tACCCGTTATCGTTCCAGGCCCTTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTTAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGAGCACATGACCGGTaa > 2:44484/1‑141 (MQ=255) gTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAaaca > 1:71044/1‑141 (MQ=255) tATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAaacaac < 1:86331/141‑1 (MQ=255) tATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAaacaac < 2:217469/141‑1 (MQ=255) gCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCtt < 2:221340/141‑1 (MQ=255) ttCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATGCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTc < 1:230794/141‑1 (MQ=255) cAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCAc < 2:186618/141‑1 (MQ=255) cAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCAc < 2:50719/141‑1 (MQ=255) gtgCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCACGATAtt < 1:29374/141‑1 (MQ=255) gtgCCAATTTCTTTT‑TGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCACGATATTg < 1:51741/141‑1 (MQ=255) gtgCCAATTTCTTTT‑TGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCACGATATTg < 1:58757/141‑1 (MQ=255) ccAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCACGATATTGtt > 1:107591/1‑141 (MQ=255) ttCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCACGATATTGTTCTGTTTGTAt > 1:184877/1‑141 (MQ=255) | ACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATATTCTAAATATTCCGTTTTGACGCCTTTAGTCCATTCGCGAACATGACCGGTAAAAACAACTACTGCACCTTGATATTCATTTATAGTGAATTCACGATATTGTTCTGTTTGTAT > CP000730/2391439‑2391711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |