Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,648,382	G→A	C108C (TGC→TGT)	lltB ←	MFS family major facilitator transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,648,382	0	G	A	100.0%	139.7 / NA	40	C108C (TGC→TGT)	lltB	MFS family major facilitator transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/16);  total (24/16)

AAGATTGGTGCCAAAGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACT  >  CP000730/2648260‑2648516                                                                                                                           |                                                                                                                                       aaGATTGGTGCCAAAGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAAt                                                                                                                      <  1:134001/141‑1 (MQ=255)  aGATTGGTGCCAAAGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATa                                                                                                                     <  2:71104/141‑1 (MQ=255)      tGGTGCCAAAGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCt                                                                                                                 <  1:159368/141‑1 (MQ=255)        gTGCCAAAGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTg                                                                                                               <  1:291589/141‑1 (MQ=255)            cAAAGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGatat                                                                                                           >  2:62223/1‑141 (MQ=255)             aaaGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGAtata                                                                                                          >  1:323010/1‑141 (MQ=255)             aaaGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGAtata                                                                                                          >  1:5499/1‑141 (MQ=255)                                 aaaTATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTAtg                                                                                      >  1:273259/1‑141 (MQ=255)                                        tGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACt                                                                               <  1:348162/141‑1 (MQ=255)                                          aTAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGc                                                                             >  2:167769/1‑141 (MQ=255)                                           tAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGca                                                                            <  2:19188/141‑1 (MQ=255)                                               tCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACaaa                                                                        >  1:319011/1‑141 (MQ=255)                                                            aGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGatat                                                           >  2:269972/1‑141 (MQ=255)                                                              ctctACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAATTTATGTGTCACTGCACAAATAAAGCTAGATATGa                                                         <  2:220304/141‑1 (MQ=255)                                                                     atgaTCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGATGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTAt                                                  <  2:353270/138‑1 (MQ=255)                                                                                 tAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGt                                      >  1:185470/1‑141 (MQ=255)                                                                                 tAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGt                                      >  2:134511/1‑141 (MQ=255)                                                                                 tAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTGGATATAGCAAATAATCGACAGTTATGTATCACTGCACAAATAAAGGCACACATGATCACTATTCCCAATCCCGt                                      >  2:273669/1‑141 (MQ=255)                                                                                             taaagttaaagtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACttt                          >  2:127262/1‑141 (MQ=255)                                                                                             taaagttaaagtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACttt                          >  2:292537/1‑141 (MQ=255)                                                                                             taaagttaaagtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAAACTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTTCCATTCCAGTTAAAAGTACttt                          >  2:79510/1‑141 (MQ=255)                                                                                                agttaaagtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACtttttt                       >  1:109831/1‑141 (MQ=255)                                                                                                 gttaaagtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTc                      <  2:319011/141‑1 (MQ=255)                                                                                                      agtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTAATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATc                 <  2:37273/141‑1 (MQ=255)                                                                                                       gtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCa                >  1:167846/1‑141 (MQ=255)                                                                                                       gtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCa                >  2:352427/1‑141 (MQ=255)                                                                                                       gtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCa                >  1:275646/1‑141 (MQ=255)                                                                                                           cGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACgag            <  2:2302/141‑1 (MQ=255)                                                                                                           cGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACgag            <  2:5499/141‑1 (MQ=255)                                                                                                           cGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACgag            >  2:226036/1‑141 (MQ=255)                                                                                                           cGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACgag            <  2:10284/141‑1 (MQ=255)                                                                                                           cGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACgag            <  2:323010/141‑1 (MQ=255)                                                                                                           cGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACgag            <  1:317719/141‑1 (MQ=255)                                                                                                              gagGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAAt         <  1:29821/141‑1 (MQ=255)                                                                                                                     aatTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACt  >  1:175137/1‑141 (MQ=255)                                                                                                                     aatTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACt  >  1:356570/1‑141 (MQ=255)                                                                                                                     aatTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACt  >  1:276994/1‑141 (MQ=255)                                                                                                                     aatTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACt  >  1:363532/1‑141 (MQ=255)                                                                                                                     aatTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACt  >  1:161867/1‑141 (MQ=255)                                                                                                                     aatTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACt  >  2:111448/1‑141 (MQ=255)                                                                                                                           |                                                                                                                                       AAGATTGGTGCCAAAGCTGGTGCACATGATAAAAATATTTGATAGTTCGCCATAGCTTGAGCTCTACCATTATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACT  >  CP000730/2648260‑2648516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑