BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,015	C→T	31.7%	R179R (AGG→AGA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,015	0	C	T	31.7%	1317.9 / inf	1078	R179R (AGG→AGA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (361/365); new base T (179/163); total (548/530)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1093 total aligned reads displayed.

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGAAACTTTGAGAc                                                                                                                                              >  1:617917/3‑141 (MQ=255)

  ttAACTTGCTCGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                                            <  1:98123/141‑1 (MQ=17)

  ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                                            >  1:147284/1‑141 (MQ=17)

     aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATCCTTTGATACCTTGt                                                                                                                                         >  2:387837/1‑141 (MQ=17)

      cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTTTTCTTTGCCGTATTGGTACTTTGATATCTTGtc                                                                                                                                        >  1:246513/1‑141 (MQ=11)

      cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtt                                                                                                                                        >  2:252492/1‑140 (MQ=255)

      cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtt                                                                                                                                        >  1:528353/1‑140 (MQ=11)

      cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATTGATCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGGTACCTTGtt                                                                                                                                        >  1:486661/1‑140 (MQ=255)

       ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                                       <  1:645583/141‑1 (MQ=17)

        tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                                      <  1:101021/141‑1 (MQ=17)

        tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                                      <  1:365024/141‑1 (MQ=17)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                     >  2:335133/1‑141 (MQ=17)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                     >  1:259957/1‑141 (MQ=17)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTATGATACCTTGtcttt                                                                                                                                     <  1:496317/141‑1 (MQ=17)

          cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttt                                                                                                                                    >  1:221003/1‑140 (MQ=17)

           tagcagtaagCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                                   <  2:548466/132‑1 (MQ=255)

           tAGCATGATGCTAATTTCGTGGCATTGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                                                                                                   <  2:277342/141‑1 (MQ=11)

           tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTGAATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGCTATCTTGtctttct                                                                                                                                   <  1:434024/141‑1 (MQ=11)

           tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTTATACTTTGATATCTTGtctttct                                                                                                                                   <  1:506123/141‑1 (MQ=11)

           tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                                   >  1:470547/1‑141 (MQ=17)

                tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                                                                              >  2:82478/1‑141 (MQ=21)

                 gatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACttt                                                                                                                             <  2:377770/141‑1 (MQ=255)

                  atgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                                                                                                                            <  1:111775/141‑1 (MQ=11)

                  atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                                                                                                                            >  1:596260/1‑141 (MQ=21)

                       aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                                       >  1:419576/1‑141 (MQ=34)

                       aaTTTCGGTGCATTGGGAAAATCCGTTGATGTGAAGATACCTACGGTTCTTTTTATATAGATCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                                                                                                                       <  2:610129/141‑1 (MQ=11)

                         tttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc                                                                                                                     <  2:596887/141‑1 (MQ=25)

                         tttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc                                                                                                                     <  1:574034/141‑1 (MQ=25)

                         gttCGGGGCATGGCGAAAATCCGTAGATCTGATGCGACCTGCTGTCCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc                                                                                                                     <  1:182404/140‑1 (MQ=11)

                          ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGGGACCTACTGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                                                                                                    <  1:88844/141‑1 (MQ=11)

                             gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACGGTg                                                                                                                 >  2:370320/1‑141 (MQ=255)

                                gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                                              >  1:351996/1‑141 (MQ=14)

                                gCATGGCGAAAATCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                                              <  2:265271/141‑1 (MQ=255)

                                  aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGACGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                                                                            <  1:627570/141‑1 (MQ=255)

                                   tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                                           <  2:148866/141‑1 (MQ=255)

                                   tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                                           <  1:133801/141‑1 (MQ=255)

                                   tGGCGAAAATCCGTAGATATGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATATTg                                                                                                           <  2:174069/141‑1 (MQ=255)

                                    ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACGTTGATATCTTGTCTTTCTTACTTTAATATGACGATGATCtgcc                                                                                                          >  2:233314/4‑138 (MQ=11)

                                         aaaTCCGTAGATCTAAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGACTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     >  1:369572/1‑141 (MQ=255)

                                            tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                                                  <  2:530023/141‑1 (MQ=255)

                                              cGTGGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTCACTACTCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                                                <  1:286915/141‑1 (MQ=11)

                                              cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                                                <  2:219334/141‑1 (MQ=255)

                                              cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                                                <  1:233451/141‑1 (MQ=255)

                                                tAGATCTGAAGAGACCTACGGTTCTTGTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                                              <  2:158951/141‑1 (MQ=255)

                                                 aGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                                                                                             >  2:50150/1‑141 (MQ=255)

                                                   aTCTTAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                                                                           <  1:564176/141‑1 (MQ=255)

                                                       gAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                                                                       <  1:272939/141‑1 (MQ=255)

                                                         agagACCTGCGGTTCTTTTTATATCGAGAGTAAATACACTCAATACCTTTTAAAGAATTCGTTGATGTATTGATACTTTCATACCTTGTCTTTCATACTTTAGTATGACGGAAATCATGCTACATGAAATTATTCAGatat                                                                                     >  2:185302/1‑141 (MQ=11)

                                                            gaCCTGCGGTTCTTTTTATATAGATCGTAAATACACTCAATACCTTTTAATGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                                                                  >  2:489986/1‑141 (MQ=255)

                                                            gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                                                                  <  2:137351/141‑1 (MQ=255)

                                                            gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                                                                  >  1:451912/1‑141 (MQ=38)

                                                             aCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGGTCTCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGATGTTATTCTGATATTTCg                                                                                 >  2:11876/1‑141 (MQ=11)

                                                                tacGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTTATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAtt                                                                              >  2:587915/3‑140 (MQ=255)

                                                                 gCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                             >  1:124660/1‑141 (MQ=37)

                                                                   ggTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAc                                                                           >  2:449686/1‑141 (MQ=255)

                                                                    gTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                                                                          >  2:517968/1‑141 (MQ=255)

                                                                      tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                        >  1:214080/1‑141 (MQ=255)

                                                                      tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                        <  1:520511/141‑1 (MQ=255)

                                                                      tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                        <  2:647100/141‑1 (MQ=38)

                                                                      tCTTTTTATATAGAGCGTAAATAACATCAATACCTTTTAAATTATTCTTTTCTTTATTGATACTTTGATACCTTGTCTTTCTTACTTGAATATGACGGTTATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                        <  2:628657/141‑1 (MQ=11)

                                                                        tttttATATAGAGCGTTAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGTTACTTTGATACCTTGTCTTTCTTACTTTCATATGACGGTGATCTTGCTCAATGATGGTATTCAGATATTTCGATGTACAATgt                                                                      >  1:329413/1‑140 (MQ=11)

                                                                        tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                                      >  1:304453/1‑141 (MQ=255)

                                                                        tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGAAACTTTTATATCTTGTCTTTCTTACTTTAATATGACGGAGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgt                                                                      >  2:301613/1‑140 (MQ=11)

                                                                        ttttgATACAGAGCGTAAATACATTCAATTCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                                      <  1:172716/141‑1 (MQ=14)

                                                                          tttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATTCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTTCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCa                                                                    >  1:78797/1‑141 (MQ=14)

                                                                            tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                  <  1:581642/141‑1 (MQ=255)

                                                                             atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATCACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTc                                                                 <  1:442275/141‑1 (MQ=255)

                                                                              tataGCGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCa                                                                <  2:636154/141‑1 (MQ=255)

                                                                              tataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTcc                                                                >  2:364714/1‑140 (MQ=255)

                                                                                taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                                              <  1:406642/141‑1 (MQ=255)

                                                                                     cGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGACATTTCGATGTACAATGACAGTCAGgtttaa                                                         <  1:659892/141‑1 (MQ=255)

                                                                                     cGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaa                                                         <  2:317222/141‑1 (MQ=255)

                                                                                        aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTAGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                      >  2:426647/1‑141 (MQ=255)

                                                                                           tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                   >  2:211166/1‑141 (MQ=40)

                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGggttaagtttaa                                                   >  2:30783/1‑141 (MQ=255)

                                                                                             cATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa                                                 >  2:352859/1‑141 (MQ=40)

                                                                                              actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAg                                                >  1:604966/3‑141 (MQ=255)

                                                                                              actCAATACCGTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGGCAGGGTTAAGTTTAAAAg                                                >  2:559822/3‑141 (MQ=255)

                                                                                                 taaTACCTTTTAAAGTATTCTTTGTCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTTAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCtt                                             <  2:662761/140‑1 (MQ=255)

                                                                                                   aTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTa                                           >  2:291006/1‑141 (MQ=255)

                                                                                                     aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                         >  1:536084/1‑141 (MQ=255)

                                                                                                     aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACACTCTATAACTTACGTGAATAGGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGAAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                         >  2:573036/1‑141 (MQ=14)

                                                                                                         tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTAAGGTTTAAGTTTAAAAGCTTTAAttctt                                     >  2:39890/1‑138 (MQ=255)

                                                                                                          ttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                                    <  1:162366/141‑1 (MQ=255)

                                                                                                          ttAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGGTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTattt                                    <  2:75151/141‑4 (MQ=255)

                                                                                                               gTATTCTTTGCTGTATTGCTGCTTTGATACCTTGTCTTTCTTAGTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTGAGATTTTTCGATGTACAATGGGAGTCAGGTTTAAGTTTGAACGCTTTAATTACTTTaatc                               >  2:91227/1‑138 (MQ=11)

                                                                                                                tATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAGAGCTTTAATTACTTTAAcct                              >  1:547455/1‑140 (MQ=255)

                                                                                                                         ttGTATTGATACTTTGATACCTTGTCTTTCTTAGTTTAATATGACGGTGATCTTGCTGAATGAGGTTATTGAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACGTTAACCATTGCTACCt                     <  1:388568/140‑1 (MQ=11)

                                                                                                                         ccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAACCATTAAATTATGTTAGCCATTGCTGCCt                     >  2:465064/3‑141 (MQ=14)

                                                                                                                         ccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTCGCCATTGCTGCCt                     >  2:112080/3‑141 (MQ=255)

                                                                                                                          tGTATTGATACTTTGATACCTTGTCTTTCTTACTTTCATATGCCGGTGCACTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCtt                    <  1:379776/141‑1 (MQ=14)

                                                                                                                           gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATTAGGTTATTCAGATATTTCGATGTACAATGGGAGGCAGGTTTAAGTTTATAAGCTTTATTTACTTTAACCTTTGCtctcttt                   >  1:151993/1‑135 (MQ=14)

                                                                                                                              ttGATACTTTGATACCTTGTCTTTCTTACTTGACTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGtt                >  2:440164/1‑141 (MQ=255)

                                                                                                                               tGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTg               <  1:196919/141‑1 (MQ=255)

                                                                                                                                gATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTCCCTTCGTTGa              >  2:201118/1‑141 (MQ=255)

                                                                                                                                     tttGATATCTTGTCTTTCTTACTTTAAGATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAGGACAGGCAGGTTTAAGTTTAAAGGCGTTAATCATGTTAACCAGTGCTGCATTCCttctcactg         >  2:478288/1‑134 (MQ=11)

                                                                                                                                      ttGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGc        <  2:62284/141‑1 (MQ=255)

                                                                                                                                       ttaTACCTTTTCTTTCTTACTTTACTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTCTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGcc       <  2:416482/139‑1 (MQ=11)

                                                                                                                                        gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCt      <  1:29345/141‑1 (MQ=255)

                                                                                                                                          tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTGATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa    >  1:394838/1‑141 (MQ=255)

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`