Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,087 A→G 60.3% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0870AG60.3% 765.3 / inf 1085C155C (TGT→TGCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (236/195);  new base G (326/324);  total (566/519)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 1092 total aligned reads displayed.

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226
                                                                                                                                            |                                                                                                                                           
  tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACa                                                                                                                                           <  1:494748/141‑1 (MQ=255)
      tataGCGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCa                                                                                                                                       <  2:636154/141‑1 (MQ=255)
      tataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTcc                                                                                                                                       >  2:364714/1‑140 (MQ=255)
         agagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCCGgt                                                                                                                                    >  1:59877/1‑141 (MQ=255)
           agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                                                                                                                  <  1:423839/141‑1 (MQ=255)
              gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                                                                                                               >  1:614472/1‑141 (MQ=255)
                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                                                                                             <  1:588752/141‑1 (MQ=255)
                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                                                                                                             >  2:358388/1‑141 (MQ=255)
                 aaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                                                                                            >  2:7070/1‑141 (MQ=39)
                  aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                                                                                           >  2:226834/1‑141 (MQ=255)
                   tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttacgtttaa                                                                                                                          >  2:300173/1‑141 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttcagtttat                                                                                                                          >  1:16758/1‑140 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGggttaagtttaa                                                                                                                          >  2:30783/1‑141 (MQ=255)
                    aCATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaa                                                                                                                         >  2:321562/1‑141 (MQ=255)
                     cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTGTAAGTTTaaaa                                                                                                                        >  1:350616/4‑141 (MQ=255)
                      actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAg                                                                                                                       >  2:441646/3‑141 (MQ=255)
                        tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCt                                                                                                                     >  2:578511/1‑141 (MQ=255)
                         taaTACCTTTTAAAGTATTCTTTGTCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTTAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCtt                                                                                                                    <  2:662761/140‑1 (MQ=255)
                           aTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGCTTTAAGTTTAAAAGCTTTa                                                                                                                  <  1:365184/141‑1 (MQ=255)
                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                                >  2:102935/1‑141 (MQ=255)
                             aCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                                >  1:50641/1‑141 (MQ=255)
                               cTTTTAAATTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                                                                                              <  2:422471/141‑1 (MQ=255)
                                ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                                                                                                             <  2:205563/141‑1 (MQ=255)
                                  ttAAAGAATTCATTGCCGTATTGATCCTTTGATATCTTATCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTAttt                                                                                                           >  2:255487/1‑141 (MQ=14)
                                   tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                                                                                          <  2:46078/141‑1 (MQ=255)
                                   tAAAGTATTCTTTGCTGTATTGATACTCTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGAGATTTCTATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                                                                                          <  1:320617/141‑1 (MQ=255)
                                    aaaGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTa                                                                                                         >  1:556844/1‑141 (MQ=255)
                                     aaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaa                                                                                                        >  1:46604/1‑140 (MQ=255)
                                        tATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAGAGCTTTAATTACTTTAAcct                                                                                                     >  1:547455/1‑140 (MQ=255)
                                         aTTCTTTTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCATTGAGGTTATTAGGATCTTACAATTTACAATGACATTCAGGTTTGAGTTTTAAACCTTTTATTTCTTTAGACAt                                                                                                    >  1:653845/1‑141 (MQ=14)
                                         aTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTACAAGCTTTAATTATTTTAGCCAt                                                                                                    >  1:83830/1‑141 (MQ=255)
                                          ttCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt                                                                                                   >  1:210343/1‑141 (MQ=255)
                                              ttGCTGTATTGATACTTTGATACCTTTTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa                                                                                               <  1:378295/141‑1 (MQ=255)
                                               tgccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtgc                                                                                              >  1:623350/5‑139 (MQ=255)
                                                 cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGCGGTTATTCAGATATTCCGATTTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                            >  1:154557/1‑141 (MQ=14)
                                                   tgaTTGATACTTTGATACCTGGTCTTTCTTACTTTAATATGACGGTGATCTTGCGCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTc                                                                                          >  2:89241/3‑141 (MQ=14)
                                                      ttGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAATCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTAGtt                                                                                       >  1:274080/1‑141 (MQ=14)
                                                      ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGtt                                                                                       <  2:149715/141‑1 (MQ=255)
                                                          tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCCACCTTCGTTGAAg                                                                                   <  1:449686/141‑1 (MQ=255)
                                                              ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATAGCTACCTTCGTTGAAGGTGc                                                                               <  1:405394/141‑1 (MQ=255)
                                                                taTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCt                                                                             <  2:551472/140‑1 (MQ=255)
                                                                gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCt                                                                             <  2:11220/141‑1 (MQ=255)
                                                                  tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa                                                                           >  1:531209/1‑141 (MQ=255)
                                                                           tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGATACCTTCGTTGAAGGTGCCTGATCTGTAAtt                                                                  <  1:389198/141‑1 (MQ=255)
                                                                           tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAtt                                                                  <  2:40478/141‑1 (MQ=38)
                                                                           tttcttACTTCAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAATTTTTAATTACTTTAGCCATTGCCCCCTTCGTTGACGGTGCCTGATCTGTAAtt                                                                  <  1:517015/141‑1 (MQ=11)
                                                                            ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAGGACAGTCAGGTTTAAGTTTAAAAGCTTTAACTACTCTAGCCATTGCTACCCTCGTTGAAGGTGCCTGATCTGTAATTa                                                                 <  2:285535/141‑1 (MQ=255)
                                                                              cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                               >  2:187634/1‑141 (MQ=255)
                                                                               ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                              >  2:374041/1‑141 (MQ=255)
                                                                               ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAGTTATTTTAGCCATTGCTGCCACCGCTGGTGTGGCCTGATCTGTAACTACCt                                                              <  2:472311/141‑1 (MQ=11)
                                                                                tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtt                                                             <  1:188043/141‑1 (MQ=255)
                                                                                tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCtt                                                             <  1:220948/141‑1 (MQ=255)
                                                                                tACTATAATATGACGGTGATCTTGCTCAGTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTGTTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTTACTACCtt                                                             >  2:242626/1‑141 (MQ=11)
                                                                                 aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCttt                                                            >  1:412659/1‑141 (MQ=255)
                                                                                     tatattGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAg                                                        <  2:594480/136‑1 (MQ=255)
                                                                                        tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTTCTACCTTCGTTTGAGGTGCCTGATCTGTATTTCACTTTTGAGGtt                                                     >  2:123353/1‑141 (MQ=11)
                                                                                         atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggctt                                                    >  1:7049/1‑138 (MQ=255)
                                                                                           gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                  <  1:540738/141‑1 (MQ=255)
                                                                                           gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                  <  1:255487/141‑1 (MQ=255)
                                                                                           gACGGTGACCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                  <  1:100669/141‑1 (MQ=255)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 >  1:19951/1‑141 (MQ=21)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 >  2:334224/1‑141 (MQ=25)
                                                                                              ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaa                                               <  1:125904/141‑1 (MQ=11)
                                                                                               gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                              <  1:284496/141‑1 (MQ=17)
                                                                                               gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCATTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                              >  2:392731/1‑141 (MQ=11)
                                                                                               aacaacatacTCAATGCGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTCAGGCTGACCaaa                                              >  2:173141/10‑141 (MQ=11)
                                                                                                   tCTTGCTCAATGAGGTTATTCAGATATTTCGAGGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                          >  2:159167/1‑141 (MQ=12)
                                                                                                    cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                         <  2:214828/141‑1 (MQ=17)
                                                                                                       gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                      >  1:438083/1‑141 (MQ=255)
                                                                                                           aaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgag                                  <  2:568885/141‑1 (MQ=11)
                                                                                                             tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTATTgagcc                                >  2:625146/1‑139 (MQ=255)
                                                                                                              gAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACg                               <  1:233015/141‑1 (MQ=11)
                                                                                                              gAGGTTATTCAGATATATCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACg                               >  1:371541/1‑141 (MQ=11)
                                                                                                                 gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAGTGAGACGttt                            <  2:451912/141‑1 (MQ=12)
                                                                                                                  ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                           >  2:381038/1‑141 (MQ=12)
                                                                                                                  ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACGAAGTTGTTTAATGAGACGTTTg                           >  2:652327/1‑141 (MQ=11)
                                                                                                                   tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGTCGTTTGa                          >  2:137515/1‑141 (MQ=11)
                                                                                                                    aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAGTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGCCGTTTGAt                         >  2:272665/1‑141 (MQ=255)
                                                                                                                     ttCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAACTTGTTTCATGAGACGTTTGATa                        >  2:81397/1‑141 (MQ=255)
                                                                                                                     ttCAGATATTTCGATGTACAATGACAGTCAGGATTAAGTTTAAAAGCTTTAATTACTTGAGCCATTGCTACCTTAGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAAACGTTTGATa                        >  1:393906/1‑141 (MQ=255)
                                                                                                                      tCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                       <  2:603793/141‑1 (MQ=17)
                                                                                                                         gATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACg                    >  1:567090/1‑141 (MQ=11)
                                                                                                                          atatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTACCTACCCTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                   <  1:292240/141‑1 (MQ=11)
                                                                                                                           tatTTCGATTGACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                  <  1:109882/141‑1 (MQ=11)
                                                                                                                              tttgATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTCAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTCATGAGACGTTTGATAAACGCatat               <  1:486223/138‑1 (MQ=12)
                                                                                                                              tgcGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat               <  1:176185/139‑1 (MQ=17)
                                                                                                                               tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAACTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg              >  2:558612/1‑141 (MQ=255)
                                                                                                                               tCGATGTACAATGACAGTTAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGAGCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg              <  1:576847/141‑1 (MQ=11)
                                                                                                                               tCGATGTACAATGACAGTCAGGTTTAAGTTTACAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGGTCTGTAACTACCTTTTGAGGCTTACAAAATAATTTAATGAGACGTTCGATAAAcacgtaaa              >  2:619189/1‑134 (MQ=11)
                                                                                                                                cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc             >  1:64087/1‑141 (MQ=17)
                                                                                                                                cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc             >  1:301793/1‑141 (MQ=17)
                                                                                                                                cGATGTACAATGGCAGTCAGGTCTAAGTTTAAAAGCTTTAATTACTTCAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAAGTGTACCAAATTGTGTAATGACACGTTGGATAAACGCatatac             >  2:486807/1‑139 (MQ=11)
                                                                                                                                 gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt            >  2:349560/1‑141 (MQ=32)
                                                                                                                                  aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg           >  2:542001/1‑141 (MQ=12)
                                                                                                                                     tACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt        <  2:499051/141‑1 (MQ=255)
                                                                                                                                     tACAATGACAGTCAGGTGTAAGTGTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGACTTACCGAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt        <  2:431535/141‑1 (MQ=11)
                                                                                                                                       cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa      >  1:13818/1‑141 (MQ=11)
                                                                                                                                        aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAAGTTTGATAAACGCATATGCTGAATggt     >  2:51315/1‑139 (MQ=2)
                                                                                                                                        aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt     <  1:247562/141‑1 (MQ=32)
                                                                                                                                         atcaCAGTCAGGTTTCAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt    <  2:528353/138‑1 (MQ=1)
                                                                                                                                            |                                                                                                                                           
TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: