Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,269 C→T 64.2% A95T (GCA→ACA)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,2690CT64.2% 522.3 / inf 1018A95T (GCA→ACA) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (178/185);  new base T (296/349);  total (476/542)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 1026 total aligned reads displayed.

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409
                                                                                                                                            |                                                                                                                                             
tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                                                                                                                               >  2:432937/1‑141 (MQ=32)
 gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                              <  2:444868/141‑1 (MQ=32)
  ctgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTACATCTGTAt                                                                                                                                             >  1:511633/4‑141 (MQ=11)
     ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                          <  2:350616/141‑1 (MQ=17)
      cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCCAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAAt                                                                                                                                         <  2:132733/141‑1 (MQ=17)
      cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAGATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                                                                                                                         <  1:126211/141‑1 (MQ=17)
              aaGGTGCCTGATCTGTAATTACCGTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGat                                                                                                                                 <  2:614731/141‑1 (MQ=17)
                 gtggcgtaTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAtata                                                                                                                              <  2:275268/134‑1 (MQ=17)
                 gTGCCTGATCTGTAACTTCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                                                                                                                              <  1:271942/141‑1 (MQ=255)
                   gCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGCATCAATGGCACGAATTaaa                                                                                                                            >  1:33703/1‑141 (MQ=12)
                      tGAACTGGAATTACATTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATGTAAATat                                                                                                                         >  2:173185/1‑140 (MQ=11)
                       gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTCTGTCCCTCTGCATCAATGGCACGATATAAATAGc                                                                                                                        >  1:110883/1‑141 (MQ=17)
                       gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCGTCTGTATCAATGGCACGATATAAATAGc                                                                                                                        >  1:463526/1‑141 (MQ=255)
                         tCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                      >  2:203892/1‑141 (MQ=12)
                         tCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                      >  1:361806/1‑141 (MQ=12)
                          ctgatactACCTTTTGATGCTTACCAAATTGTTTAATGAGACGTTTGTAATATGAATGTTCTGAATGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                                                                                                                     <  1:624470/134‑1 (MQ=11)
                          cTGTAATTCCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc                                                                                                                     <  2:627492/141‑1 (MQ=17)
                           tGTAACTACCTTTTCAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                    <  2:123105/141‑1 (MQ=255)
                           tGTAAATACTTTTTGAGTTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCACGTTACTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                    <  2:524309/141‑1 (MQ=11)
                            tttaTTACCTTTTTAGGTTTTCCAAATTGTTTATTGAGACGTTTTATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                                                                                                                   <  1:233921/138‑1 (MQ=11)
                             tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGACACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGCTATAAATAGCTCCAtt                                                                                                                  <  1:369615/141‑1 (MQ=17)
                              aaTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt                                                                                                                 >  1:158526/1‑141 (MQ=17)
                               aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTTCATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                                >  1:77742/1‑141 (MQ=17)
                               aTTACCTTTTGAGGTTTACCAAAATGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                                >  2:476854/1‑141 (MQ=17)
                                ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                               <  2:154557/141‑1 (MQ=17)
                                ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCCATGGCACGATATAAATAGCTCCATTTTc                                                                                                               >  1:546724/1‑141 (MQ=17)
                                ctACCTTTTGAGGCTTACTAATTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                               <  1:37723/140‑1 (MQ=255)
                                   ccTTTTGAGGTTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                            <  2:596285/141‑1 (MQ=17)
                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                           <  1:353475/141‑1 (MQ=32)
                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                           >  1:93805/1‑141 (MQ=32)
                                    cTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTTCGCAACCAAATATCTAATGTTTGTCCCTCTTTATCAATGGCACGCTATAAAAAGCTCCATTTTCCttt                                                                                                           >  1:259448/1‑141 (MQ=11)
                                     ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTGGCTTACGCAACCAAATATCTAATCTATGTCCCTCTGTATCAAGGGCACTATATAAATAGCTCCATTTTCCtttt                                                                                                          <  2:241311/141‑1 (MQ=11)
                                      tttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTTAATGATTCTATAGATTCTTACGCAACCAAATATCTACATTAGGGCCCTCCGTATCAATGGCACGATTTAAAAAGCACCATGTTCCgtttt                                                                                                         >  2:487884/1‑140 (MQ=2)
                                      tttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTa                                                                                                         <  1:401531/141‑1 (MQ=17)
                                      tttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAACGATTATCTCGTTGCTTACGCAACCAAATATCTATTTTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTa                                                                                                         <  2:366326/141‑1 (MQ=12)
                                          aggcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGCCACGATCTAAATAGCTCCATTTTCCTTTTAtttt                                                                                                     >  2:226750/5‑141 (MQ=255)
                                            gTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCTACCAAATATCTAATGTATGTCCCTCTGCATCATTGGCACGATATAAATAGCTCGATTTTCCTTTTAtttttt                                                                                                   >  1:18013/1‑139 (MQ=12)
                                             tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                  <  2:638824/141‑1 (MQ=32)
                                             cttACCAACTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                  <  1:392731/140‑1 (MQ=12)
                                              ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAta                                                                                                 >  1:309940/1‑140 (MQ=17)
                                                     aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtatagttct                                                                                          >  1:594143/1‑136 (MQ=255)
                                                     aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtct                                                                                          <  1:616664/141‑1 (MQ=12)
                                                         ttgactgaagcGTTTGATCAACGCATCGTGTGAATGATTATGGCGTTGCTTACGCAACCAATTATCTAATGTATGGCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatc                                                                                      <  2:580150/131‑1 (MQ=11)
                                                           tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATCaa                                                                                    >  1:100662/1‑141 (MQ=12)
                                                           tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCaa                                                                                    >  1:188133/1‑141 (MQ=32)
                                                              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                <  2:37196/141‑1 (MQ=17)
                                                                 gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATGCGcc                                                                             >  1:638707/1‑141 (MQ=17)
                                                                  aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCaaaaagcaa                                                                             >  1:282558/1‑134 (MQ=17)
                                                                      ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                        >  2:166355/1‑141 (MQ=17)
                                                                         ttAATCGGATATGCTTAATGTTTATCTCCTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCCAGGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                     <  2:547455/140‑1 (MQ=11)
                                                                         atatACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACAAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                     <  2:53314/141‑1 (MQ=17)
                                                                         aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                     >  2:334069/1‑141 (MQ=32)
                                                                           aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                   <  1:248403/141‑1 (MQ=16)
                                                                             aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAg                                                                 <  2:417585/141‑1 (MQ=255)
                                                                               gCATATGTTGAATGATTATCTCGTTGCTTACGCAACCAAATATTTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCt                                                               <  2:65039/141‑1 (MQ=255)
                                                                                 atatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCttt                                                             <  2:571307/141‑1 (MQ=25)
                                                                                  tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTTTTATAAGCtttt                                                            >  1:474758/1‑141 (MQ=255)
                                                                                  tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            >  2:460939/1‑141 (MQ=34)
                                                                                   atGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCttttt                                                           >  2:359920/1‑141 (MQ=25)
                                                                                    tGCTTAATTATTATCTGGTTGCTTACGTAACCAAATATCTAATGTATGTCCCTCGGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          <  1:587165/141‑1 (MQ=11)
                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATGAGCtttttt                                                          >  1:296806/1‑141 (MQ=12)
                                                                                     gcctaaTGATTATCTCTCTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                         <  2:317812/137‑1 (MQ=255)
                                                                                      cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCATTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCACCAATCCGCCATTTGTAATAAGCTTTTTTAt                                                        >  1:535239/1‑141 (MQ=11)
                                                                                       tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                       >  2:101977/1‑141 (MQ=255)
                                                                                        gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAGGCTTTTTTATGc                                                      <  1:522253/141‑1 (MQ=255)
                                                                                          aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtt                                                    <  2:382697/141‑1 (MQ=255)
                                                                                           tGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttt                                                   <  1:441646/141‑1 (MQ=255)
                                                                                            gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAtttgttt                                                  >  1:14796/1‑135 (MQ=11)
                                                                                              ttATCTCGTTGCTTACTCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                <  1:85006/141‑1 (MQ=255)
                                                                                               tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               <  2:555118/141‑1 (MQ=40)
                                                                                                       tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                       <  2:114672/141‑1 (MQ=255)
                                                                                                       tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACATCTCATCAATAAGCCATTTGTAATAAGCTTTATTTTGTATTttcttcttactt                                       >  1:576128/1‑135 (MQ=11)
                                                                                                       tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATATCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCTAAAtt                                       >  1:663807/1‑141 (MQ=255)
                                                                                                       tGCTTACGCAACCAAAGATCTAATGTATGTCCCTCTGTATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                       <  1:500931/141‑1 (MQ=255)
                                                                                                         cTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAGttta                                     >  1:566319/1‑140 (MQ=255)
                                                                                                          ttACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTTTTTCAAttttta                                    >  1:215665/1‑139 (MQ=14)
                                                                                                           tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCAAAGTTGAt                                   >  1:195704/1‑141 (MQ=255)
                                                                                                            aCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                  >  2:255872/1‑141 (MQ=255)
                                                                                                            aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                  >  2:40503/1‑141 (MQ=40)
                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:203892/141‑2 (MQ=255)
                                                                                                              gcatcaacatgtCTAATGTATGTCCGTCTTGACCGATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                <  1:650730/130‑3 (MQ=14)
                                                                                                              gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTtatata                                >  2:498676/1‑139 (MQ=255)
                                                                                                                       tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       <  1:451948/141‑1 (MQ=255)
                                                                                                                       tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       <  1:609665/141‑1 (MQ=38)
                                                                                                                       ggtCTAATGTACGTCCCTCTGCAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGATATAAAATTgggg                       <  2:538093/139‑1 (MQ=11)
                                                                                                                         tCTAATTGATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                     <  2:288934/141‑1 (MQ=255)
                                                                                                                          cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGGGGCat                    <  1:330806/141‑1 (MQ=255)
                                                                                                                           tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTTTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   >  1:383813/1‑141 (MQ=11)
                                                                                                                           tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   <  2:301793/141‑1 (MQ=255)
                                                                                                                           tAATGTATGTCCCTCTGCATCAATGGCACGATATACATAGCTCCATTTTCCTTTTATTTTGATGTACTTCTCATCAATACGCCATTAGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAATTGGGGCatt                   >  1:117354/1‑140 (MQ=11)
                                                                                                                             atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAtttt                 >  2:182924/1‑141 (MQ=255)
                                                                                                                                  tgtCCCCCTGTATCCATGGCACGATATAAATAGCTCCTTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa            <  1:167056/141‑1 (MQ=11)
                                                                                                                                  tgtCACTCTGTATCAATGGCACGATATAAATAGAACCATCTACCTTTTTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAATTTGGGGGATATTCTTGa            >  1:319659/1‑141 (MQ=11)
                                                                                                                                    tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACCACCAAGTTGATAAGATTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc          >  1:484825/1‑141 (MQ=11)
                                                                                                                                     cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc         <  2:78235/141‑1 (MQ=21)
                                                                                                                                      ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGTGGCATATTCTTGAAccc        >  2:391656/1‑141 (MQ=11)
                                                                                                                                      ccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc        <  1:556823/141‑1 (MQ=21)
                                                                                                                                          tGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGGACCCAACg    >  1:42401/1‑141 (MQ=255)
                                                                                                                                           tcATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg   <  2:659342/140‑1 (MQ=18)
                                                                                                                                           gCATCAATGGCACGATATAAATTGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg   <  2:88435/141‑1 (MQ=18)
                                                                                                                                            |                                                                                                                                             
TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: