BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	57.0%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	57.0%	268.9 / inf	1025	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (229/200); new base A (260/314); total (502/523)
Fisher's exact test for biased strand distribution p-value = 1.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 1030 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTT  >  CP000730/36175‑36454

          gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                                                                   <  2:549655/141‑1 (MQ=17)

          gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                                                                   <  2:616322/141‑1 (MQ=17)

             tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATCaa                                                                                                                                >  1:100662/1‑141 (MQ=12)

             tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtct                                                                                                                                      >  1:645269/1‑135 (MQ=33)

               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAACGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                                                              <  2:470214/141‑1 (MQ=12)

                tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                             <  2:133151/141‑1 (MQ=17)

                   gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCGCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGct                                                                                                                          <  2:173742/141‑2 (MQ=12)

                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATATTCGTCTCATCAATGCGCCa                                                                                                                         >  1:551877/1‑141 (MQ=11)

                     cGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAt                                                                                                                        <  1:465707/141‑1 (MQ=17)

                         tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTTTGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTTTCATCAATACGCCATTTGt                                                                                                                    >  1:258131/1‑141 (MQ=17)

                          gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAAGCCATTTgca                                                                                                                   >  1:270466/1‑139 (MQ=17)

                           aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATTTCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                  <  2:525630/141‑1 (MQ=12)

                           aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                  <  1:62033/141‑1 (MQ=12)

                            tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaat                                                                                                                 <  1:178928/141‑1 (MQ=32)

                            tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCAAATTgacct                                                                                                                 >  2:164115/1‑137 (MQ=12)

                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                                                                <  1:529737/141‑1 (MQ=17)

                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                                                                <  1:189656/141‑1 (MQ=18)

                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                                                                >  2:659089/1‑141 (MQ=18)

                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                                                                <  2:536257/141‑1 (MQ=32)

                               aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAg                                                                                                              <  1:558323/141‑1 (MQ=21)

                                cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATCTCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                                                                             >  1:292194/1‑141 (MQ=255)

                                      tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTACTTTGATATACGTCTCATCAATACGTCATTTGTAATAAGCtttttt                                                                                                       <  2:556844/141‑1 (MQ=11)

                                      tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                                                                       <  1:358388/141‑1 (MQ=255)

                                      tGCTGAATGATTATCTCGTTGCTTACGCAACCAAAGATCTAATGTATGTAAATCTGAATCAATCGCACGATATAATTTGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                                                                       <  2:569013/141‑1 (MQ=11)

                                          gAATGATTATCTCGTTGCTTACGCAACTAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                                                                   <  1:576185/141‑1 (MQ=255)

                                           aaTGATTATCTCGTTGCTTACGCAACCACATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTNTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                                                                  <  1:379754/141‑1 (MQ=255)

                                           aaTGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                                                                  <  2:59877/141‑1 (MQ=255)

                                            aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGCCATTTGTGATAAGCTTTTTTATGCtt                                                                                                 >  1:613031/1‑141 (MQ=255)

                                               aatagctcGTTGCTTACACAACCAATTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCAGCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                                                              <  2:259448/136‑1 (MQ=14)

                                               aTTATCTCGGTGATTACTCAACCATATATCTAATGTATTTCCCTCGTCATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                                                              <  2:80585/141‑1 (MQ=11)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTGTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTttctt                                                                                           >  2:330576/1‑141 (MQ=255)

                                                    ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                                                                         <  1:455874/141‑1 (MQ=40)

                                                      cggtGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCCATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaa                                                                                       <  2:45539/138‑1 (MQ=255)

                                                      cGTTGCTTACGCAACCAAATATTTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaa                                                                                       <  1:479388/141‑1 (MQ=255)

                                                        ttGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAt                                                                                     <  2:418930/141‑1 (MQ=39)

                                                         tGCTTACGCAACCAAAGATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACAtt                                                                                    <  2:114692/141‑1 (MQ=255)

                                                          gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAtttt                                                                                   >  1:8715/1‑141 (MQ=14)

                                                             tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAt                                                                                <  1:78279/141‑1 (MQ=255)

                                                             tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAt                                                                                <  2:364521/141‑1 (MQ=255)

                                                              aCGCAACCAAATTTCTAATGTATGTCCTTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                                                               <  2:650058/141‑1 (MQ=255)

                                                              aCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAACTCTTTTATGCTTTTTCTTCCAATTTTGATa                                                                               >  1:326466/1‑141 (MQ=11)

                                                               cGCAACCAAATATCTAATGTATGTCCTTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACCTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                              <  1:226928/141‑2 (MQ=255)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                             >  1:664592/1‑139 (MQ=255)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                            >  2:542702/1‑141 (MQ=255)

                                                                    ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                         >  2:339627/1‑141 (MQ=255)

                                                                    caaaaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                         <  1:446934/139‑1 (MQ=255)

                                                                     cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTCTACTTCTCATCAATACGCCATTTGTAATAAGCTTCTTTCTGCTTTTTCTTGCAAAtttagtgcataatt                                                                        >  1:12436/1‑130 (MQ=11)

                                                                      aaaTATCTAATATATGTCCCTCTGTCTCCAAGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAGTTTTATTTAAAATTg                                                                       >  2:440436/1‑141 (MQ=11)

                                                                         tatCTAATGTATGTTCCTCTGTATCAATGGCACGATTTAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATTCGCCATTTGTAATATGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                    <  1:540465/141‑1 (MQ=11)

                                                                         tatCTAATGTATGTCCCTCTGTATCAAGGGCACGACCTAAATAGCTCCTTTGGCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                    <  2:234212/141‑1 (MQ=11)

                                                                         tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTgggg                                                                    <  2:19951/141‑1 (MQ=255)

                                                                           tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                  >  2:102359/1‑141 (MQ=255)

                                                                            tttatggatgtCCCTCTGCATCAATGGCACGATATAAATAGCCCCATGTTCCTTTTATTTTGATTTACGTCTCAGCTATACGTCATTTGTAAGAGTTTTTTTTATGCTTTTTCGTCCAAATTTGGTTCAAAATTGGGGCat                                                                 <  2:186905/138‑1 (MQ=14)

                                                                            cgaaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                 <  1:280122/139‑1 (MQ=255)

                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATACATAGCTCCATTTTCCTTTTATTTTGATGTACTTCTCATCAATACGCCATTAGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAATTGGGGCatt                                                                >  1:117354/1‑140 (MQ=11)

                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                <  2:353776/141‑1 (MQ=37)

                                                                              aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                               >  1:523506/1‑141 (MQ=255)

                                                                              aaTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                               >  2:636747/1‑141 (MQ=38)

                                                                                    tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTATATGCTTTTTCTTCCAAATTTGATATAAAATTGGGACATCTTTTTGa                                                         >  2:316969/1‑141 (MQ=11)

                                                                                    tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCATTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATTGGGGCAGATTTTTGa                                                         >  1:507576/1‑141 (MQ=255)

                                                                                    tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                         <  1:40503/141‑1 (MQ=25)

                                                                                       ccatctGTATCAAGGGCACGATATAAATAGCTGCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc                                                      <  1:165820/138‑1 (MQ=11)

                                                                                        ccTCTGCATCACTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAAccc                                                     <  1:314041/141‑1 (MQ=255)

                                                                                           ttGCATCAATGGCACGATATTAATAGCTTCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                  <  1:346468/140‑1 (MQ=255)

                                                                                           ctgtaacAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                  <  1:395193/135‑1 (MQ=255)

                                                                                            tGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGGACCCAACg                                                 >  1:42401/1‑141 (MQ=255)

                                                                                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCGTTTGTAATAAGCTTTTTTATGCATTTTCTTCCAAATTTGATATAAAAGTGGGAGATATTGTTTAAACCAACGGCAg                                             >  1:200572/1‑141 (MQ=11)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTACAATTTTCGTTCTATGTTTGAAATAAAATTGGGGAATTTTCTTGAATCCAGCGGTTGa                                            >  1:651788/1‑141 (MQ=11)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTTTTGAACTCAACGGtcgt                                            >  2:585660/1‑138 (MQ=11)

                                                                                                   aTGGCACGATATAAATAGCACCATTTTCGTTTTATTTTGTTATACGGCTGATCAATTCTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatc                                          >  1:632552/1‑139 (MQ=11)

                                                                                                    tGGCACGATATAAATATCTCTATTTTCCTTTTATTTTGATGTTCGTCTTATCAATACGTCATTTGTTATAAGCTTTTTTATGCTTTTTCTTCCAAAGTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                         <  1:586464/141‑1 (MQ=11)

                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATTGGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCAAAATTTGATTTAAAATTCTGGCATATTCTTGAACCCAAGGGTAGGCCGt                                        >  1:94379/1‑141 (MQ=11)

                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                        <  2:292194/141‑1 (MQ=255)

                                                                                                       taCGACATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                      <  2:394813/140‑1 (MQ=19)

                                                                                                       cACGATATAAATAGCTCCATCTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                      <  2:485776/141‑1 (MQ=255)

                                                                                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                    >  2:91864/1‑141 (MQ=255)

                                                                                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                    >  1:242182/1‑141 (MQ=255)

                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                 <  2:248785/141‑1 (MQ=255)

                                                                                                             ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                <  2:124901/141‑1 (MQ=40)

                                                                                                                aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                             >  1:1437/1‑141 (MQ=21)

                                                                                                                 aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTACCAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTACAATTGGTGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                            >  1:156670/1‑141 (MQ=11)

                                                                                                                  tAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATTGTTGAATGATGAACg                           <  1:494316/141‑1 (MQ=255)

                                                                                                                      tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                       <  2:371541/141‑1 (MQ=255)

                                                                                                                       ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTac                      >  1:387580/1‑141 (MQ=255)

                                                                                                                        cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTcca                     >  2:379057/1‑141 (MQ=255)

                                                                                                                         aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                    >  2:207029/1‑141 (MQ=40)

                                                                                                                         aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                    <  1:436620/141‑1 (MQ=255)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                   >  2:514247/1‑141 (MQ=255)

                                                                                                                           ttgtcTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                  <  2:404015/137‑1 (MQ=19)

                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                 <  2:152699/141‑1 (MQ=255)

                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                 >  2:279967/1‑141 (MQ=255)

                                                                                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGt               >  1:218275/1‑141 (MQ=25)

                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt              >  1:591789/1‑141 (MQ=255)

                                                                                                                                ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGTACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtct             >  2:49752/1‑139 (MQ=19)

                                                                                                                                ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc             <  1:206879/141‑1 (MQ=255)

                                                                                                                                 tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACATTTACACGCCGTTcc            >  2:387680/1‑141 (MQ=11)

                                                                                                                                     ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCttt        <  1:572626/141‑2 (MQ=11)

                                                                                                                                     ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa        >  1:368303/1‑141 (MQ=255)

                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat    >  2:625368/1‑141 (MQ=21)

                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat    >  1:333463/1‑141 (MQ=25)

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTT  >  CP000730/36175‑36454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`