Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,817 A→T 45.1% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8170AT45.1% 127.3 / 136.6 117intergenic (+48/+60)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (37/27);  new base T (15/38);  total (52/65)
Fisher's exact test for biased strand distribution p-value = 1.58e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35677‑35956
                                                                                                                                            |                                                                                                                                           
gCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGTTCTAATAAAAATAGaa                                                                                                                                             >  2:531005/1‑141 (MQ=255)
       attCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTg                                                                                                                                      >  2:512420/1‑141 (MQ=255)
          cTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTg                                                                                                                                   >  1:296030/1‑141 (MQ=255)
           tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGc                                                                                                                                  >  1:162033/1‑141 (MQ=255)
           tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGc                                                                                                                                  >  1:220818/1‑141 (MQ=255)
           tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGATATTCAGTATAAAGTTTTTGTTGAATAATGACTACTTGAACAAGATCTAAAAGAAAAATTTGATCGAATAAAAATAGAAGGTTCTGTTGc                                                                                                                                  >  2:157358/1‑141 (MQ=255)
                     gATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGGaaaaa                                                                                                                        >  2:321017/1‑141 (MQ=255)
                      aTGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaaaa                                                                                                                       >  2:177163/1‑141 (MQ=255)
                       tGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaaaaa                                                                                                                      <  1:665204/141‑1 (MQ=255)
                                tctcATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTaa                                                                                                             <  1:338017/141‑1 (MQ=255)
                                tctcATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTaa                                                                                                             >  2:175675/1‑141 (MQ=255)
                                  tcATTTAAAAAGTATTTAATGTATAAGCTATTCAGTATAAATTTTTTGGCGAAATATGATTACTTGAACAAGATCTAAAATAAAAGTGTGATCTAAGAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAcc                                                                                                           <  1:219702/141‑1 (MQ=38)
                                                 ttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGt                                                                                            >  1:576591/1‑141 (MQ=255)
                                                  tAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTc                                                                                           <  1:139311/141‑1 (MQ=255)
                                                   aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                          >  2:556568/1‑141 (MQ=255)
                                                   aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAAAAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                          >  1:553800/1‑141 (MQ=255)
                                                      gTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtg                                                                                       >  2:241817/1‑141 (MQ=255)
                                                           aGCTAATAAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                  <  2:562515/141‑1 (MQ=255)
                                                            gCTATTAAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCt                                                                                 <  2:383991/141‑1 (MQ=255)
                                                                 tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                            <  1:175675/141‑1 (MQ=255)
                                                                   aGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAATAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTg                                                                          >  1:162583/1‑141 (MQ=255)
                                                                      ataAAGTTTTTGGCGAAAAATGATTACTTGGACAAGAGCTAAAATAAACTTGTGATCTAATAGAAATATAAGGTTCTATTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTTAGTGTTCGCTTAACTTGCTa                                                                       <  2:1971/141‑1 (MQ=25)
                                                                      aaaaacttataggCGAAAAATGATTACTTCAACAAGAGCTAAAATAAAAATTTTATCAAATAAATATATAATGTATTTTTAAAAAATAAAAAAATATAGCTAACCACTAATTAATCATGTCAGTGTTCGCTTAACTTGCTa                                                                       <  2:359211/130‑1 (MQ=12)
                                                                            tttttGGCGAAAAATGATTACTTGAACAAGGGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGcctca                                                                 >  2:564176/1‑137 (MQ=255)
                                                                             ttttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgat                                                                <  1:358663/141‑1 (MQ=255)
                                                                             ttttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgat                                                                <  1:214941/141‑1 (MQ=255)
                                                                              tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAACTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                               <  1:539352/141‑1 (MQ=255)
                                                                              tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                               >  2:192729/1‑141 (MQ=255)
                                                                               ttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGc                                                              <  1:139033/141‑1 (MQ=255)
                                                                                 ggCGAAAAATGATTACTTGAACAAGAGCTAAAATACAATTGTGAACTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGATCACTAACAATTGCTCATGTAAGTATACGCTTAGCTTGCTAGAGTGATGCTa                                                            >  2:398352/1‑141 (MQ=21)
                                                                                   cGAAAAATGATTACTTGAACAAGAGCTAAAGTAAAATTGTAATCTAATAAACATAGAAGGTTATGTTGCAAAGAAAAAAAATATAGCAAACGACTGATTTATCATTAGAGTGTTCGCTTAACTTGCTATCATAGTACTAAt                                                          >  2:176185/1‑141 (MQ=21)
                                                                                      aaaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGGTACTAATTTc                                                       >  1:107462/1‑141 (MQ=255)
                                                                                      aaaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATACTAATTTc                                                       >  1:139794/1‑141 (MQ=255)
                                                                                       aaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTAGATCAACTTGCTAGCATGATGCTAATTTCg                                                      <  1:159721/141‑1 (MQ=255)
                                                                                        aaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTATGTTGCAAAGTAAAAAAATATAGATAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGATAGGATCATGTTAATTTCGt                                                     >  2:385892/1‑141 (MQ=21)
                                                                                               aCTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATATATCATGTCTATGACCACGGAGCTTGCTAGCATGATGCTAATTTCGTGGCATgg                                              <  1:657845/141‑1 (MQ=21)
                                                                                                cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                             >  2:583744/1‑141 (MQ=255)
                                                                                                    aaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaa                                         <  2:162033/141‑1 (MQ=255)
                                                                                                    aaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTACCTTGGCATGGTTCGATCAACGGTGTAGCATGATGCTAATTTCGTGGCATGGCGaaa                                         <  1:157358/141‑1 (MQ=18)
                                                                                                    aaCAAGAGCTAAAATAAAATCGTGATCTAATAAAAATAGAAGGTTAAGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAGTTTCGTGGCATGGAGaaa                                         >  2:508716/1‑141 (MQ=18)
                                                                                                        agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                     >  1:667232/1‑141 (MQ=255)
                                                                                                          agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                   <  1:192729/141‑1 (MQ=255)
                                                                                                            cTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTAAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCCAAAAACCTTac                                 <  2:576591/141‑2 (MQ=255)
                                                                                                             taaaataaaaTTGTGATCTAATAAAAATAGGAGGTTCTGTTGCAAAGTCAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGGGCAATTGAGTTGCTAGCATGATGCTAACTTCGTGGCAAGGGGAAAATTCGTAGa                                >  1:453624/1‑141 (MQ=17)
                                                                                                             taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                >  1:537439/1‑141 (MQ=255)
                                                                                                                 acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                          >  2:147566/15‑141 (MQ=11)
                                                                                                                  caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTACTCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         <  1:168558/128‑1 (MQ=11)
                                                                                                                  caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAGATCCGTAGATCTGAag                         <  1:396503/128‑1 (MQ=11)
                                                                                                                  caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         <  1:350364/128‑1 (MQ=11)
                                                                                                                  caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         <  1:289535/128‑1 (MQ=11)
                                                                                                                  aaaaatagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         <  1:456635/128‑1 (MQ=11)
                                                                                                                   aaaagagtattttaataaAAATGATGGTTCTGTTGTTAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        <  1:513680/129‑1 (MQ=11)
                                                                                                                    caatTGTGATCTACTAAAAGTAAAAGGTCCTGGTGCAAATTAGAAAAATATAGCTGTACACTAATTTATGATGTCAGTGTTCGGTAAACTTGCTAGTAAGATTCTAATTTCGTGGCATGGCGAAAATCCGTGGTTCTGaga                         >  2:322313/2‑139 (MQ=9)
                                                                                                                    aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                       >  1:104310/12‑141 (MQ=11)
                                                                                                                    aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                       >  1:641607/12‑141 (MQ=11)
                                                                                                                    aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGAGGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                       >  2:536122/12‑141 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATTGCTAATCACTAATTTATCATGTCAGTTTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  2:296497/132‑1 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     >  1:603793/10‑141 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:658730/132‑1 (MQ=11)
                                                                                                                      agactattttaataaACATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTCAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  2:347629/132‑1 (MQ=11)
                                                                                                                       aaagaatttaataaAAATGATGATTCTGTGTCAAAGAAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  2:106640/133‑1 (MQ=11)
                                                                                                                        tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTCATTTATCATGTCAGTGTGCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:254931/141‑1 (MQ=255)
                                                                                                                        agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                   <  1:611719/134‑1 (MQ=11)
                                                                                                                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  1:437345/135‑1 (MQ=11)
                                                                                                                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  1:552215/135‑1 (MQ=11)
                                                                                                                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  2:107916/135‑1 (MQ=11)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:663003/136‑1 (MQ=11)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:541181/136‑1 (MQ=11)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  2:280475/136‑1 (MQ=11)
                                                                                                                          tattttaataaAAATGATGATTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:635151/136‑1 (MQ=11)
                                                                                                                           gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  >  1:554976/1‑141 (MQ=255)
                                                                                                                           ccgtcatattaaaTAGCTGGTTCTGTTCCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCtac                 <  1:527764/131‑3 (MQ=11)
                                                                                                                           atttttataaAAATGATGGTTCTGTTGCGAAGTAAAAAATATAGCTAATCTCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                <  2:27776/135‑1 (MQ=11)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                >  2:442275/5‑141 (MQ=11)
                                                                                                                           atttgaagaaaaaTGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                <  2:454307/133‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATTGTTCTGTTCCAAAGTAAAAAATATAGCTAAACACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  2:135722/138‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGGGACATGCgg               >  2:275677/4‑141 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  1:438781/138‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCgg               >  1:638948/4‑141 (MQ=11)
                                                                                                                            agtcatattaaaTGGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCACGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCtacg                <  2:436980/132‑4 (MQ=11)
                                                                                                                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:531005/141‑1 (MQ=255)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCGTGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTGCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:555063/139‑1 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGAGCTGAAGAGACCTGCGGt              <  2:144755/139‑1 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              >  2:394143/3‑141 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  1:106057/139‑1 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCCTTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:362816/139‑1 (MQ=11)
                                                                                                                             tttaagaaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:374664/139‑1 (MQ=11)
                                                                                                                             tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                <  1:512420/141‑1 (MQ=255)
                                                                                                                               taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc            >  2:290786/1‑141 (MQ=11)
                                                                                                                                aataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAATAATTTATCATGTCAGTATTCGCTTAACTTGCTAGCATGAAGCTAATTTCGTGGCACGGCACACATCCGTAGACCTGAAGTGAGCcgcgcgc             >  2:348392/1‑138 (MQ=14)
                                                                                                                                aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt           >  2:269111/1‑141 (MQ=11)
                                                                                                                                aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt           >  2:29345/1‑141 (MQ=11)
                                                                                                                                 ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt          >  1:567860/1‑141 (MQ=11)
                                                                                                                                     aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt        >  2:598979/1‑141 (MQ=35)
                                                                                                                                     aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt        >  2:55809/1‑141 (MQ=35)
                                                                                                                                      aaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCGCTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttt       <  2:357565/141‑1 (MQ=21)
                                                                                                                                         aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata    >  1:1103/1‑141 (MQ=32)
                                                                                                                                         aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata    >  1:567056/1‑141 (MQ=32)
                                                                                                                                           aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata  <  1:125442/141‑1 (MQ=11)
                                                                                                                                           aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGCCGAAAATCCGTAGATCTGAAGAGACCTGCGTTTCtttttcttta  >  1:231785/1‑136 (MQ=2)
                                                                                                                                            |                                                                                                                                           
GCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35677‑35956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: