Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,413 C→T 46.6% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4130CT46.6% 139.6 / inf 573V47I (GTC→ATC) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (169/132);  new base T (133/132);  total (309/264)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

Only 100 of 579 total aligned reads displayed.

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAC  >  CP000730/36273‑36553
                                                                                                                                             |                                                                                                                                            
  tGGCACGATATAATTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                                                            <  1:14631/141‑1 (MQ=18)
    gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATCAAATTGGGGCCTCTTCTTGAACCCAAAAGTAGACCGtt                                                                                                                                          >  1:582690/1‑141 (MQ=11)
    gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGtt                                                                                                                                          >  1:238599/1‑141 (MQ=21)
    gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGtt                                                                                                                                          >  2:631270/1‑141 (MQ=21)
      aCGATTTACATGGCTCCATTTTCCTGTTATTTTGATGTACTTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCATAGTTGATTTATAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                        <  2:33703/141‑1 (MQ=11)
      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGTTTTTTTTTCCAAATTTGATATAAAATTGGGGTATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                        >  2:47462/1‑141 (MQ=11)
      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                        >  1:444989/1‑141 (MQ=25)
       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                                                       >  1:17557/1‑141 (MQ=255)
       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCAGTTTATAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTAAGGCATATTCTTGAACCCAACGGTAGAACGTTGaa                                                                                                                                       >  1:647994/1‑141 (MQ=11)
        gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                                                                      >  1:423485/1‑141 (MQ=255)
         atatAAATTGTCCCATTTTCCTTTTACTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                                     <  2:259350/141‑1 (MQ=11)
         atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                                                                     <  2:32706/141‑1 (MQ=21)
          tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                                    <  1:360396/141‑1 (MQ=255)
          gataAATTTCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                                    >  2:660845/2‑141 (MQ=11)
           ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                                                                                                                   <  2:106249/141‑1 (MQ=40)
            taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAACGttatataatg                                                                                                                                  >  2:62041/1‑133 (MQ=11)
             aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTAATATAAAATTGAGGCATAATCTTGAACCCAACGTTAGCCCGTTGAAtgatga                                                                                                                                 >  2:221585/1‑141 (MQ=11)
             aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCCTTTTCTTCCAAATCTGATACAAAATTGGGGCACAGTCCTAACCCCAACGGCAGACCGTTGACtgatga                                                                                                                                 >  2:157664/1‑141 (MQ=14)
               aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTACCAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTACAATTGGTGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                                                                                                                               >  1:156670/1‑141 (MQ=11)
                tAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCTATTCGCCACTTAGAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACg                                                                                                                              <  1:51315/141‑1 (MQ=11)
                 aGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAAGGTAGGCCGTGGAATGATGAACGt                                                                                                                             <  1:661431/141‑1 (MQ=255)
                  ggtCCATTTTCCTTGTATTTTGATCTACGTACCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                                                            <  2:13325/139‑1 (MQ=11)
                  gCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                                                                                                                               >  1:55613/1‑138 (MQ=21)
                  gCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                                                                                                                               <  2:55613/138‑1 (MQ=21)
                  gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                                                            <  2:443732/141‑1 (MQ=255)
                    tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTTAACCCAACGGAAGATCGTTGATTGTTGAACGtttt                                                                                                                          >  2:243465/1‑140 (MQ=11)
                    tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                                                          >  2:498164/1‑141 (MQ=255)
                    tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                                                          <  1:26721/141‑1 (MQ=255)
                     ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                                                                                                                         >  2:535492/1‑141 (MQ=21)
                      cATTTTCTTTTTATTTTGATGTACGTCTCAATAATACGTCTTTTGGAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                                                                                                                        <  1:123353/141‑1 (MQ=11)
                      cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTcca                                                                                                                        >  2:379057/1‑141 (MQ=255)
                      cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                        >  1:162140/1‑141 (MQ=255)
                       aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                       <  2:563621/141‑1 (MQ=21)
                       aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                       >  2:207029/1‑141 (MQ=40)
                        ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                                                      >  2:481845/1‑141 (MQ=40)
                         tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                                                     >  2:599878/1‑141 (MQ=25)
                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                                                     <  1:537691/141‑1 (MQ=255)
                          ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCAACGGTCGACCGTTGATCGATGaaagattcaacctc                                                                                                                    <  2:389651/141‑13 (MQ=11)
                            ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGt                                                                                                                  >  1:218275/1‑141 (MQ=25)
                             cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                                                 >  1:564664/1‑141 (MQ=21)
                             cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                                                 >  1:137707/1‑141 (MQ=25)
                             cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGGTGAATGATGAACGTTTACACCACgat                                                                                                                 >  2:295042/1‑139 (MQ=255)
                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                                 >  1:529243/1‑141 (MQ=255)
                              ttttatttttatGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTc                                                                                                                <  2:93805/141‑1 (MQ=18)
                                ttATTTTGATATACGTCTCCTCAATACGCCGTTTGTAATAAGCTCTTTTATGCTTTTTATTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGGTAAATGATGAACGATTaaagccacttctc                                                                                                              >  2:54495/1‑129 (MQ=11)
                                ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                                                              <  2:590916/141‑3 (MQ=255)
                                  atttgcaTGTACGTCTCATCAATATGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                            <  2:45936/135‑1 (MQ=11)
                                  aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                            <  1:91864/141‑1 (MQ=11)
                                     ttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat                                                                                                         <  1:224667/141‑1 (MQ=18)
                                       tatatccGTTTCATCAATTCGCCACTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                       <  1:61956/135‑1 (MQ=11)
                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                       >  1:119626/5‑141 (MQ=11)
                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                       >  1:473646/5‑141 (MQ=11)
                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAAACCACGttatcataatct                                                                                                       >  2:126915/5‑131 (MQ=11)
                                       gatatACGTCTCATCAATAAGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATGGTTGAATGATAAACGTTTAAACAACGTTCTCTTAatat                                                                                                       >  2:361192/5‑141 (MQ=11)
                                       gacatACGTCTCACCAATACGCCATTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGATCCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                       <  2:19163/137‑1 (MQ=11)
                                       gATGTACGTCTCATCAATACACCATTTGAAATAAGCTTTTTTATGCTTTTTCTTGCAAATTTGATATAAAATTGGGGCAGATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                       >  1:430610/1‑141 (MQ=11)
                                           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGTCCGTTGAATGAGGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                   <  2:112714/141‑1 (MQ=255)
                                            aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                  <  2:638674/141‑1 (MQ=255)
                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAgt                                                                                                 >  1:277428/1‑140 (MQ=255)
                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                                                 <  1:249611/141‑1 (MQ=255)
                                              gTCTGATCAATACGCCATTTGAAATAAGCTTTGTTATGCGTTTTATTCCAAATTTGATATAAAATTGCGGCAGCTTCTTGAACCCAACGGTAGATCGATGAGTGATGAACGTTCACACCGTGTACTCATAATCTTTCAgta                                                                                                >  2:83555/1‑139 (MQ=2)
                                              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                <  1:635427/141‑1 (MQ=255)
                                              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                >  1:393923/1‑141 (MQ=255)
                                                ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                                                              <  1:54999/141‑1 (MQ=255)
                                                ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatat                                                                                              >  2:614662/1‑141 (MQ=21)
                                                 tcaccaATATGCCATTTGTAATAAGCCTTTTTAGGCTTTTTCTTCCAAATTCGATATAAATATTGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGCTCTCTTAATATTTCAGatata                                                                                             <  1:344190/141‑1 (MQ=11)
                                                  catcgATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACAGTTGAATGATGAACGTTTACACCACGGTCCCTTAATATTTCAGatatat                                                                                            >  2:403309/1‑141 (MQ=255)
                                                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                            >  2:42801/1‑141 (MQ=255)
                                                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatatat                                                                                            <  1:213488/141‑1 (MQ=18)
                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTGGTCTAAATTTGTGACATAATTCGACATGATTCTTGAACCCAACGGTATAGCGTTGAATGATGAACGTTTACACCACGTCCCCTTAAATTTTCAGATATATc                                                                                           >  2:273991/1‑141 (MQ=14)
                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                           >  2:148620/1‑141 (MQ=25)
                                                    tcaATACGCCATTTGTAATAGGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAACTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCATGTTCTCTTAATATTTCAGATATATCa                                                                                          >  1:402920/1‑141 (MQ=11)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCGCGTTCCCTTATTTTTTCAAatccagcc                                                                                          >  2:30690/1‑135 (MQ=11)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                          <  1:32539/141‑1 (MQ=255)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                          >  1:46622/1‑141 (MQ=255)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                          >  2:649262/1‑141 (MQ=255)
                                                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                         <  2:280174/141‑1 (MQ=255)
                                                      aaTACGCCATTTATAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGAGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAGACCACGTTCTCTTAATATTTCAGATATATCACg                                                                                        >  2:370939/1‑141 (MQ=11)
                                                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                       <  1:576129/141‑1 (MQ=255)
                                                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAGTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCActa                                                                                       >  1:457574/1‑139 (MQ=255)
                                                       aTACACCATTTGTAATAAGCTTTTTTTGGCTTTTTCTTCCAAATTTGATATAAAATGGGGGCATATTCTTAAACCCAACGGTAGATCGTTGAATGATGAACGCTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                       <  2:511633/141‑1 (MQ=11)
                                                         aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                     <  1:596854/141‑1 (MQ=255)
                                                         aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                     >  1:190011/1‑141 (MQ=255)
                                                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                    >  1:386545/1‑140 (MQ=255)
                                                           gCCATTTGTAATAAGCTTTTTTAGGCGTTTTCTTCCAAATTTGATATAAGATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                   <  1:299116/141‑3 (MQ=11)
                                                             cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                 >  2:355511/1‑141 (MQ=25)
                                                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATAACGATAGCTCa                                                                                >  1:129490/1‑141 (MQ=11)
                                                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                >  1:320481/1‑141 (MQ=25)
                                                               tttGTAATAATCTTTTCTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATCCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                               <  1:49771/141‑1 (MQ=11)
                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                   >  2:188025/1‑133 (MQ=255)
                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                             <  2:580205/141‑1 (MQ=18)
                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGAGATATCACGATAACTCAATgca                                                                           <  2:170774/141‑3 (MQ=12)
                                                                               ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                                               <  1:100280/141‑1 (MQ=35)
                                                                                           ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCt                                                   <  1:349945/141‑1 (MQ=35)
                                                                                             ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACCCAATGTATATCTTAGATAGTAGCCAACGGCTAc                                                 >  1:197381/1‑141 (MQ=25)
                                                                                             ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTAAAATATATCACGATAACTCAATGTATATCTTAGATGGTAGCCAACGGCTAc                                                 >  1:286692/1‑141 (MQ=255)
                                                                                              cAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACa                                                >  2:519093/1‑141 (MQ=35)
                                                                                                                                 cccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa              <  1:37194/141‑1 (MQ=32)
                                                                                                                                       ggTAGAGCGTTGAATGATGAACGTTTACAGCCCGTGCCCTAAATATTTCAGATATATCACGATAACACAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTCGTTAAATTGTTTATATCTGAAATAGt        >  1:489431/1‑141 (MQ=11)
                                                                                                                                             ccGTTGAATGATGAACGTTTACTCCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAc  <  1:167627/141‑1 (MQ=17)
                                                                                                                                             |                                                                                                                                            
AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAC  >  CP000730/36273‑36553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: