Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,471 A→G 22.0% S27S (AGT→AGC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4710AG22.0% 224.3 / 61.7 139S27S (AGT→AGCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (51/57);  new base G (13/18);  total (64/75)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 142 total aligned reads displayed.

GCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTAT  >  CP000730/36332‑36611
                                                                                                                                             |                                                                                                                                            
gCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATAATGAACGTTTACACCACGTTCCCTTAGTATTCCAGATATATCACGATAAc                                                                                                                                             <  1:128837/141‑1 (MQ=11)
gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                                                             >  1:124922/1‑141 (MQ=21)
gCCATTTGTAATAAGCTTTTTTATGCGTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                                                             <  1:158398/141‑1 (MQ=18)
 ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                                            >  1:105576/1‑141 (MQ=255)
 ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                                            >  1:268128/1‑141 (MQ=255)
 ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGCTAAcc                                                                                                                                            >  2:647836/1‑140 (MQ=19)
 ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                                            >  2:595669/1‑141 (MQ=21)
 ccATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAAGTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGAAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCGGATATATCACGATAACt                                                                                                                                            >  1:426839/1‑141 (MQ=11)
  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAAGTTGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                           <  2:47083/141‑1 (MQ=11)
  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                           <  2:66244/141‑1 (MQ=11)
  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                           <  2:31454/141‑1 (MQ=255)
  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                           <  1:228831/141‑1 (MQ=18)
  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                           >  2:355511/1‑141 (MQ=25)
  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATAACGATAACTc                                                                                                                                           >  1:323577/1‑141 (MQ=21)
   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                                                          >  2:206630/1‑141 (MQ=25)
    tttGTAATCAGCTTTTTTATGCTTTTTCTTCCCAATTTGATCTACTATTATGGCATACTCCTGAGCCCAACGGTTGACCGTTGAATGATGAACGGTTACACCACGCTCCCTTAAAATTTCAGATATATCACGATAACTcca                                                                                                                                         >  2:304558/1‑139 (MQ=11)
    tttGTAATAATCTTTTCTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATCCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                         <  1:49771/141‑1 (MQ=11)
     ttttaataaGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                        <  2:349696/138‑1 (MQ=11)
     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATATTATTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                        <  2:200333/141‑1 (MQ=11)
     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                        <  1:445465/141‑1 (MQ=21)
     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                        >  1:134731/1‑141 (MQ=21)
     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                        <  1:396555/141‑1 (MQ=21)
     ttGTAAAAAGCTTTTTTATGCTTTTTCTACCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                        <  2:503362/141‑1 (MQ=255)
      tttaataaGTTTTTTTATGCTTTTTCTTCCAAAGTGGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                       <  2:240638/139‑1 (MQ=255)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAtc                                                                                                                                       >  2:28471/1‑140 (MQ=255)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                       <  1:659163/141‑1 (MQ=255)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                       <  1:323834/141‑1 (MQ=11)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                       <  2:535239/141‑1 (MQ=255)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                       <  2:32369/141‑1 (MQ=21)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                       <  2:35781/141‑1 (MQ=21)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                       <  2:148808/141‑1 (MQ=255)
       ggaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCTTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                      <  1:227807/139‑2 (MQ=255)
       gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                      >  2:121550/1‑140 (MQ=16)
       gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGATAACTCAATgc                                                                                                                                      >  2:86735/1‑140 (MQ=12)
       gTAATAAGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                      >  1:306230/1‑140 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     <  2:299070/141‑3 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     <  2:282562/141‑3 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                                                     <  1:350882/141‑3 (MQ=17)
        taataaGCTTTTTTATGCTGTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACAGCACGGTGCCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     >  2:138969/1‑139 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGAAATATCACGATAGCCCAAtccct                                                                                                                                    >  2:187439/1‑137 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcaa                                                                                                                                    >  2:591847/1‑138 (MQ=11)
          ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCATTGtata                                                                                                                                   >  2:54104/1‑141 (MQ=255)
          ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtcta                                                                                                                                   >  2:378104/1‑141 (MQ=255)
                 tttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACAAAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGa                                                                                                                            >  1:625013/1‑141 (MQ=25)
                    ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                                                                                                         <  1:100280/141‑1 (MQ=35)
                    ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATAACTTAGAtag                                                                                                                         >  1:597979/1‑141 (MQ=21)
                            tttATTGCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATGTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAAc                                                                                                                 <  1:157664/141‑1 (MQ=255)
                              tcttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACgg                                                                                                               >  2:547771/1‑141 (MQ=35)
                              tcttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACgg                                                                                                               <  2:77742/141‑1 (MQ=35)
                                ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCt                                                                                                             <  1:349945/141‑1 (MQ=35)
                                  ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAAATAGTAGCCAACggatgc                                                                                                           >  2:478196/1‑137 (MQ=25)
                                  ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACCCAATGTATATCTTAGATAGTAGCCAACGGCTAc                                                                                                           >  1:197381/1‑141 (MQ=25)
                                  ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTAAAATATATCACGATAACTCAATGTATATCTTAGATGGTAGCCAACGGCTAc                                                                                                           >  1:286692/1‑141 (MQ=255)
                                   cAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACa                                                                                                          >  2:519093/1‑141 (MQ=35)
                                        ttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTATAGTGAt                                                                                                     <  2:368021/141‑1 (MQ=25)
                                        ttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAt                                                                                                     <  1:265276/141‑1 (MQ=35)
                                        ttGATACAAAATTGGGGAATTGTTCTTAACCCAACGGTAGCTAGACCGAACGGGAACGTTCACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAt                                                                                                     <  1:651257/141‑1 (MQ=11)
                                         tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCATAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa                                                                                                    >  1:465783/1‑141 (MQ=25)
                                            tACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACa                                                                                                 >  2:563279/1‑141 (MQ=35)
                                            tACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAATTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCCACGGCTACAGTGCTAACa                                                                                                 >  2:482998/1‑141 (MQ=255)
                                            tACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACAGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAcc                                                                                                 >  2:110381/1‑140 (MQ=21)
                                                 aaTTGGTGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAAGACCGTTTTCACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCtt                                                                                            <  2:270466/141‑1 (MQ=255)
                                                    tGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTGACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGtt                                                                                         <  1:123703/141‑1 (MQ=17)
                                                        gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                                                     <  1:207029/141‑1 (MQ=32)
                                                          atatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTg                                                                                   >  1:263384/1‑141 (MQ=32)
                                                            tatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGCTAACATCCTTGTTCAATTGt                                                                                  >  1:599675/1‑141 (MQ=17)
                                                               tCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTta                                                                               <  2:577180/141‑1 (MQ=32)
                                                                    aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGATAACTCAATGTATCTCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTCAATTGTTTATATCt                                                                          >  2:24713/1‑141 (MQ=12)
                                                                    aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                          <  1:519093/141‑1 (MQ=32)
                                                                    aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                          >  1:551794/1‑141 (MQ=32)
                                                                      cccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa                                                                        <  1:37194/141‑1 (MQ=32)
                                                                            ggTAGAGCGTTGAATGATGAACGTTTACAGCCCGTGCCCTAAATATTTCAGATATATCACGATAACACAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTCGTTAAATTGTTTATATCTGAAATAGt                                                                  >  1:489431/1‑141 (MQ=11)
                                                                                gACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATAAAGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAAATCCTTGTTAAATTGTTTATATCTGAACTAGttact                                                              >  1:136728/1‑138 (MQ=11)
                                                                                  ccGTTGAATGATGAACGTTTACTCCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAc                                                            <  1:167627/141‑1 (MQ=17)
                                                                                    gTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACag                                                          >  2:591781/1‑141 (MQ=32)
                                                                                    gTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACag                                                          >  1:667046/1‑141 (MQ=32)
                                                                                    gTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTCTTTATATCTCAAATAGTTCATACag                                                          <  1:334069/141‑1 (MQ=17)
                                                                                         aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaaga                                                      <  1:355511/141‑1 (MQ=32)
                                                                                               gaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCtt                                                >  1:29860/1‑141 (MQ=32)
                                                                                                aaCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttt                                               <  2:258131/141‑1 (MQ=32)
                                                                                                aaCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttt                                               <  2:275691/141‑1 (MQ=32)
                                                                                                     ttACACCACGTTCCCTTAATATTTCCGCTATATTACGATCACTCAATGTAGATCTTCGATAGTAGCCAACGGCTACAGGGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACGGAAGACTCCTTTTTGtt                                          <  1:137284/141‑1 (MQ=11)
                                                                                                       acacCACGTTCCCTTAATATTTCAGATATATGCCGATAACTCAGTGTATGTCTTAGATAGTAGCCAACGGCTACAGTGATGACAGCCTTGTGAAATTGTTTATATCTGCAATAGTTCATACAGAATCAGCATTTTTGTTaa                                        >  2:282174/1‑141 (MQ=11)
                                                                                                         accacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaaaa                                      >  2:530222/1‑141 (MQ=32)
                                                                                                                    ttAATATTTCAGATATATCACGCTAACTCAATGTATAACCTAGATAGTAGCCAACGGCTACAGTGTTAACATCCTTGTCAAATTGTTTATATCTAAAATAGTTCATACAGAAGTGTCGTTTTTGTTAAAATAGTACTATaa                           >  2:48732/1‑141 (MQ=11)
                                                                                                                       atctttCAGCCATATCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACTGCTACAGTGATAACATCCTTGTTAAATTGTTCATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAAtt                        <  1:554685/138‑1 (MQ=1)
                                                                                                                        tatTTCAGATATATCACGATAGCTCAACGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTc                       <  1:385854/141‑1 (MQ=255)
                                                                                                                         atTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                      <  1:201487/141‑1 (MQ=35)
                                                                                                                          tttCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCaa                     <  2:496702/141‑1 (MQ=35)
                                                                                                                          ttacAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCaa                     <  1:478306/138‑1 (MQ=25)
                                                                                                                            tCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACt                   >  1:174276/1‑141 (MQ=35)
                                                                                                                            tCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACt                   >  1:461650/1‑141 (MQ=35)
                                                                                                                                   tagcACGATAACTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAc            <  2:183814/138‑1 (MQ=2)
                                                                                                                                     tCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAg          >  1:288255/1‑141 (MQ=35)
                                                                                                                                       aCGATAACTCAATGTATATCTTAGATAGTAGACAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTTTTTTTTTCTATAAATTCGACTTTTGATCAGaa        >  2:140991/1‑141 (MQ=11)
                                                                                                                                         gATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcc      >  2:550425/1‑141 (MQ=34)
                                                                                                                                          aTAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCg     >  1:573116/1‑141 (MQ=35)
                                                                                                                                           tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt    <  1:638325/141‑1 (MQ=35)
                                                                                                                                           tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTCTGCAACAGAACCGt    <  1:356719/141‑1 (MQ=37)
                                                                                                                                             aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTCAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAACGtat  >  1:486273/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTAT  >  CP000730/36332‑36611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: